Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome

Our indoor microbiome consists of a wide range of microbial taxa. Whilst many of these microbes are benign, some are beneficial, some harmful, yet our knowledge of the spatial heterogeneity of bacterial assemblages in our residential environment remains limited. To investigate the existence of a com...

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Autores principales:	Jonathan R. Thompson, Ariadne Argyraki, Matthew Bashton, Lindsay Bramwell, Matthew Crown, Andrew S. Hursthouse, Khadija Jabeen, Paula Marinho Reis, Anil Namdeo, Andrew Nelson, David A. Pearce, Sanja Potgieter-Vermaak, Pat E. Rasmussen, Joanna Wragg, Jane A. Entwistle
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	vacuum dust bacterial assemblage citizen science 16S microbiome Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/2d018521645647c3bb17de65a10df8b5
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spelling	oai:doaj.org-article:2d018521645647c3bb17de65a10df8b52021-11-30T12:51:21ZBacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome2296-665X10.3389/fenvs.2021.754657https://doaj.org/article/2d018521645647c3bb17de65a10df8b52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fenvs.2021.754657/fullhttps://doaj.org/toc/2296-665XOur indoor microbiome consists of a wide range of microbial taxa. Whilst many of these microbes are benign, some are beneficial, some harmful, yet our knowledge of the spatial heterogeneity of bacterial assemblages in our residential environment remains limited. To investigate the existence of a common core house dust bacterial microbiome we selected household vacuum dusts, collected through a citizen science approach, from homes across two bioclimatic regions (UK, Oceanic/Maritime and Greece, Mediterranean). Following the extraction of DNA from each dust sample, we targeted the bacterial 16S rRNA gene using Illumina NextSeq sequencing. PERMANOVA analysis of the microbial communities at family level grouped samples within their distinct bioclimatic region and SIMPER analysis at genus level identified the statistically significant taxa responsible for driving diversity between these groups. A “common to all” core house dust microbiome consisted of Acinetobacter, Massalia, Rubellimicrobium, Sphingomonas and Staphylococcus; genera typically associated with human occupancy and common environmental sources. Additionally, a “unique location specific” microbiome was identified, reflective of the bioclimatic region. The Greek dusts indicated a lower average diversity than the UK house dusts, with a high abundance of Rhizobiaceae in the Greek samples. Our study highlights citizen science as a powerful approach to access the indoor residential environment, at scale, and establishes the existence of a “core” house dust microbiome independent of bioclimatic region.Jonathan R. ThompsonAriadne ArgyrakiMatthew BashtonLindsay BramwellMatthew CrownAndrew S. HursthouseKhadija JabeenPaula Marinho ReisAnil NamdeoAndrew NelsonDavid A. PearceSanja Potgieter-VermaakPat E. RasmussenJoanna WraggJane A. EntwistleFrontiers Media S.A.articlevacuum dustbacterial assemblagecitizen science16SmicrobiomeEnvironmental sciencesGE1-350ENFrontiers in Environmental Science, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vacuum dust bacterial assemblage citizen science 16S microbiome Environmental sciences GE1-350
spellingShingle	vacuum dust bacterial assemblage citizen science 16S microbiome Environmental sciences GE1-350 Jonathan R. Thompson Ariadne Argyraki Matthew Bashton Lindsay Bramwell Matthew Crown Andrew S. Hursthouse Khadija Jabeen Paula Marinho Reis Anil Namdeo Andrew Nelson David A. Pearce Sanja Potgieter-Vermaak Pat E. Rasmussen Joanna Wragg Jane A. Entwistle Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome
description	Our indoor microbiome consists of a wide range of microbial taxa. Whilst many of these microbes are benign, some are beneficial, some harmful, yet our knowledge of the spatial heterogeneity of bacterial assemblages in our residential environment remains limited. To investigate the existence of a common core house dust bacterial microbiome we selected household vacuum dusts, collected through a citizen science approach, from homes across two bioclimatic regions (UK, Oceanic/Maritime and Greece, Mediterranean). Following the extraction of DNA from each dust sample, we targeted the bacterial 16S rRNA gene using Illumina NextSeq sequencing. PERMANOVA analysis of the microbial communities at family level grouped samples within their distinct bioclimatic region and SIMPER analysis at genus level identified the statistically significant taxa responsible for driving diversity between these groups. A “common to all” core house dust microbiome consisted of Acinetobacter, Massalia, Rubellimicrobium, Sphingomonas and Staphylococcus; genera typically associated with human occupancy and common environmental sources. Additionally, a “unique location specific” microbiome was identified, reflective of the bioclimatic region. The Greek dusts indicated a lower average diversity than the UK house dusts, with a high abundance of Rhizobiaceae in the Greek samples. Our study highlights citizen science as a powerful approach to access the indoor residential environment, at scale, and establishes the existence of a “core” house dust microbiome independent of bioclimatic region.
format	article
author	Jonathan R. Thompson Ariadne Argyraki Matthew Bashton Lindsay Bramwell Matthew Crown Andrew S. Hursthouse Khadija Jabeen Paula Marinho Reis Anil Namdeo Andrew Nelson David A. Pearce Sanja Potgieter-Vermaak Pat E. Rasmussen Joanna Wragg Jane A. Entwistle
author_facet	Jonathan R. Thompson Ariadne Argyraki Matthew Bashton Lindsay Bramwell Matthew Crown Andrew S. Hursthouse Khadija Jabeen Paula Marinho Reis Anil Namdeo Andrew Nelson David A. Pearce Sanja Potgieter-Vermaak Pat E. Rasmussen Joanna Wragg Jane A. Entwistle
author_sort	Jonathan R. Thompson
title	Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome
title_short	Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome
title_full	Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome
title_fullStr	Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome
title_full_unstemmed	Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome
title_sort	bacterial diversity in house dust: characterization of a core indoor microbiome
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2d018521645647c3bb17de65a10df8b5
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Bacterial Diversity in House Dust: Characterization of a Core Indoor Microbiome

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