Existence of seven human IL-1R1 promoters

Qiming Li, Hao Zhang, Qun Chen, Ning QuanDepartment of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Previous studies have reported the existence of three promoters for the human type I interleukin-1 receptor (hIL-1R1) gene. These promoters were discovered by identifying discrete t...

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Autores principales:	Qiming Li, Hao Zhang, Qun Chen, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2d027eafdea348debc88cd45488c2fcb
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spelling	oai:doaj.org-article:2d027eafdea348debc88cd45488c2fcb2021-12-02T00:43:37ZExistence of seven human IL-1R1 promoters1178-7031https://doaj.org/article/2d027eafdea348debc88cd45488c2fcb2010-02-01T00:00:00Zhttp://www.dovepress.com/existence-of-seven-human-il-1r1-promoters-a4007https://doaj.org/toc/1178-7031Qiming Li, Hao Zhang, Qun Chen, Ning QuanDepartment of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Previous studies have reported the existence of three promoters for the human type I interleukin-1 receptor (hIL-1R1) gene. These promoters were discovered by identifying discrete transcription start sites (TSS) from limited human cell lines. In this study, we examined the TSSs of hIL-1R1 mRNA from 24 different tissues and identified several novel TSSs in hIL-1R1 that suggest the existence of seven hIL-1R1 promoters: three of them are the same as those reported previously and four are putative novel promoters. Using a promoter-reporter assay, we show here that these promoters can drive the transcription of the reporter gene. In addition, these promoters exhibit cell type specific expression patterns and they can be regulated by enhancer elements in a cell type specific manner. Only one of the promoters was found to be sensitive to the stimulation by glucocorticoids. Similar to our recent report on murine IL-1R1, two of the hIL-1R1 promoters appear to be the dominant promoters, one of which was published previously and the other is identified in the present study. We also found an internal promoter that drives the expression of IL-1R1 after the conventional translation start codon, suggesting that a truncated hIL-1R1 may be expressed by this promoter. These results provide additional information regarding the transcription of hIL-1R1.Keywords: type1 interleukin receptor, human IL-1R1 promoters, transcription start sites, reporter gene, stress hormones, acute phase proteins Qiming LiHao ZhangQun Chenet alDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2010, Iss default, Pp 17-24 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Qiming Li Hao Zhang Qun Chen et al Existence of seven human IL-1R1 promoters
description	Qiming Li, Hao Zhang, Qun Chen, Ning QuanDepartment of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Previous studies have reported the existence of three promoters for the human type I interleukin-1 receptor (hIL-1R1) gene. These promoters were discovered by identifying discrete transcription start sites (TSS) from limited human cell lines. In this study, we examined the TSSs of hIL-1R1 mRNA from 24 different tissues and identified several novel TSSs in hIL-1R1 that suggest the existence of seven hIL-1R1 promoters: three of them are the same as those reported previously and four are putative novel promoters. Using a promoter-reporter assay, we show here that these promoters can drive the transcription of the reporter gene. In addition, these promoters exhibit cell type specific expression patterns and they can be regulated by enhancer elements in a cell type specific manner. Only one of the promoters was found to be sensitive to the stimulation by glucocorticoids. Similar to our recent report on murine IL-1R1, two of the hIL-1R1 promoters appear to be the dominant promoters, one of which was published previously and the other is identified in the present study. We also found an internal promoter that drives the expression of IL-1R1 after the conventional translation start codon, suggesting that a truncated hIL-1R1 may be expressed by this promoter. These results provide additional information regarding the transcription of hIL-1R1.Keywords: type1 interleukin receptor, human IL-1R1 promoters, transcription start sites, reporter gene, stress hormones, acute phase proteins
format	article
author	Qiming Li Hao Zhang Qun Chen et al
author_facet	Qiming Li Hao Zhang Qun Chen et al
author_sort	Qiming Li
title	Existence of seven human IL-1R1 promoters
title_short	Existence of seven human IL-1R1 promoters
title_full	Existence of seven human IL-1R1 promoters
title_fullStr	Existence of seven human IL-1R1 promoters
title_full_unstemmed	Existence of seven human IL-1R1 promoters
title_sort	existence of seven human il-1r1 promoters
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/2d027eafdea348debc88cd45488c2fcb
work_keys_str_mv	AT qimingli existenceofsevenhumanil1r1promoters AT haozhang existenceofsevenhumanil1r1promoters AT qunchen existenceofsevenhumanil1r1promoters AT etal existenceofsevenhumanil1r1promoters
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Existence of seven human IL-1R1 promoters

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