Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis

Long non-coding RNAs (lncRNAs) are closely associated with the development of lung adenocarcinoma (LADC). The present study focused on the role of LINC00960 in LADC. miRNA and mRNA expression levels were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular...

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Autores principales:	Zhipeng Ge, Haibo Liu, Tao Ji, Qiaoling Liu, Lulu Zhang, Pengchong Zhu, Liang Li, Liangming Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	linc00960 lung adenocarcinoma aggressiveness sphk1 mir-124a Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2d052a2cdd7e4e2daf630c6b8dc821f3
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spelling	oai:doaj.org-article:2d052a2cdd7e4e2daf630c6b8dc821f32021-11-11T14:23:43ZLong non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis2165-59792165-598710.1080/21655979.2021.1996507https://doaj.org/article/2d052a2cdd7e4e2daf630c6b8dc821f32021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1996507https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Long non-coding RNAs (lncRNAs) are closely associated with the development of lung adenocarcinoma (LADC). The present study focused on the role of LINC00960 in LADC. miRNA and mRNA expression levels were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular functions were evaluated by MTT, colony formation, and Transwell assays, respectively. LINC00960 Luciferase and RNA pull-down assays were performed to clarify the interaction between miR-124a and LINC00960 or Recombinant Sphingosine Kinase 1 (SphK1). We observed that LINC00960 was overexpressed in LADC tumor tissues and cell lines. LINC00960 knockdown suppressed the proliferation, migration, and invasion of LADC cells. Moreover, LINC00960 sponged miR-124a to inhibit the SphK1/S1P pathway in LADC cells. LINC00960 knockdown markedly reduced the rate of tumor growth. The luciferase reporter assay results demonstrated an interaction between miR-124a and LINC00960 or SphK1. This interaction was confirmed using the RNA pull-down assay. In addition, miR-124a downregulation or SphK1 upregulation reversed the inhibitory effects of LINC00960 knockdown on cellular functions of LADC cells, suggesting that LINC00960 may be a potential therapeutic biomarker for LADC via the miR-124a/SphK1 axis. Accordingly, LINC00960 may be a potential therapeutic biomarker for LADC.Zhipeng GeHaibo LiuTao JiQiaoling LiuLulu ZhangPengchong ZhuLiang LiLiangming ZhuTaylor & Francis Grouparticlelinc00960lung adenocarcinomaaggressivenesssphk1mir-124aBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	linc00960 lung adenocarcinoma aggressiveness sphk1 mir-124a Biotechnology TP248.13-248.65
spellingShingle	linc00960 lung adenocarcinoma aggressiveness sphk1 mir-124a Biotechnology TP248.13-248.65 Zhipeng Ge Haibo Liu Tao Ji Qiaoling Liu Lulu Zhang Pengchong Zhu Liang Li Liangming Zhu Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis
description	Long non-coding RNAs (lncRNAs) are closely associated with the development of lung adenocarcinoma (LADC). The present study focused on the role of LINC00960 in LADC. miRNA and mRNA expression levels were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular functions were evaluated by MTT, colony formation, and Transwell assays, respectively. LINC00960 Luciferase and RNA pull-down assays were performed to clarify the interaction between miR-124a and LINC00960 or Recombinant Sphingosine Kinase 1 (SphK1). We observed that LINC00960 was overexpressed in LADC tumor tissues and cell lines. LINC00960 knockdown suppressed the proliferation, migration, and invasion of LADC cells. Moreover, LINC00960 sponged miR-124a to inhibit the SphK1/S1P pathway in LADC cells. LINC00960 knockdown markedly reduced the rate of tumor growth. The luciferase reporter assay results demonstrated an interaction between miR-124a and LINC00960 or SphK1. This interaction was confirmed using the RNA pull-down assay. In addition, miR-124a downregulation or SphK1 upregulation reversed the inhibitory effects of LINC00960 knockdown on cellular functions of LADC cells, suggesting that LINC00960 may be a potential therapeutic biomarker for LADC via the miR-124a/SphK1 axis. Accordingly, LINC00960 may be a potential therapeutic biomarker for LADC.
format	article
author	Zhipeng Ge Haibo Liu Tao Ji Qiaoling Liu Lulu Zhang Pengchong Zhu Liang Li Liangming Zhu
author_facet	Zhipeng Ge Haibo Liu Tao Ji Qiaoling Liu Lulu Zhang Pengchong Zhu Liang Li Liangming Zhu
author_sort	Zhipeng Ge
title	Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis
title_short	Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis
title_full	Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis
title_fullStr	Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis
title_full_unstemmed	Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis
title_sort	long non-coding rna 00960 promoted the aggressiveness of lung adenocarcinoma via the mir-124a/sphk1 axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/2d052a2cdd7e4e2daf630c6b8dc821f3
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Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis

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