The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor
Summary: The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2). However, the role of glycosylation has not been fully understood. In...
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2021
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oai:doaj.org-article:2d0562f6489549838ba1b3d9e2be89f32021-11-20T05:09:08ZThe effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor2589-004210.1016/j.isci.2021.103272https://doaj.org/article/2d0562f6489549838ba1b3d9e2be89f32021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012414https://doaj.org/toc/2589-0042Summary: The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2). However, the role of glycosylation has not been fully understood. In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2. Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells. Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2. The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.Chuncui HuangZeshun TanKeli ZhaoWenjun ZouHui WangHuanyu GaoShiwei SunDongbo BuWengang ChaiYan LiElsevierarticleProteinVirologyStructural biologyComputer simulationScienceQENiScience, Vol 24, Iss 11, Pp 103272- (2021) |
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DOAJ |
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Protein Virology Structural biology Computer simulation Science Q |
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Protein Virology Structural biology Computer simulation Science Q Chuncui Huang Zeshun Tan Keli Zhao Wenjun Zou Hui Wang Huanyu Gao Shiwei Sun Dongbo Bu Wengang Chai Yan Li The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor |
| description |
Summary: The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2). However, the role of glycosylation has not been fully understood. In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2. Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells. Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2. The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models. |
| format |
article |
| author |
Chuncui Huang Zeshun Tan Keli Zhao Wenjun Zou Hui Wang Huanyu Gao Shiwei Sun Dongbo Bu Wengang Chai Yan Li |
| author_facet |
Chuncui Huang Zeshun Tan Keli Zhao Wenjun Zou Hui Wang Huanyu Gao Shiwei Sun Dongbo Bu Wengang Chai Yan Li |
| author_sort |
Chuncui Huang |
| title |
The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor |
| title_short |
The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor |
| title_full |
The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor |
| title_fullStr |
The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor |
| title_full_unstemmed |
The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor |
| title_sort |
effect of n-glycosylation of sars-cov-2 spike protein on the virus interaction with the host cell ace2 receptor |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/2d0562f6489549838ba1b3d9e2be89f3 |
| work_keys_str_mv |
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