Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.

This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic...

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Autores principales: Jeffrey D Howard, Harshini Sarojini, Rong Wan, Sufan Chien
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Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/2d0cb78c5d9d49ddb90b495a3c6ab888
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spelling oai:doaj.org-article:2d0cb78c5d9d49ddb90b495a3c6ab8882021-11-18T08:27:51ZRapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.1932-620310.1371/journal.pone.0091787https://doaj.org/article/2d0cb78c5d9d49ddb90b495a3c6ab8882014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24637626/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic and four on the normal ear. Two wounds on one side of each ear were treated with Mg-ATP encapsulated lipid vesicles (ATP-vesicles) while the two wounds on the other side were treated with Regranex. Wound healing time was shorter when ATP-vesicles were used. The most striking finding was that new tissue growth started to appear in less than 1 day when ATP-vesicles were used. The growth continued and covered the wound area within a few days, without the formation of a provisional matrix. Regranex-treated wounds did not have this growth pattern. In wounds treated by ATP-vesicles, histologic studies revealed extremely rich macrophage accumulation, along with active proliferating cell nuclear antigen (PCNA) and positive BrdU staining, indicating in situ macrophage proliferation. Human macrophage culture suggested direct collagen production. These results support an entirely new healing process, which seems to have combined the conventional hemostasis, inflammation, and proliferation phases into a single one, thereby eliminating the lag time usually seen during healing process.Jeffrey D HowardHarshini SarojiniRong WanSufan ChienPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91787 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jeffrey D Howard
Harshini Sarojini
Rong Wan
Sufan Chien
Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
description This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic and four on the normal ear. Two wounds on one side of each ear were treated with Mg-ATP encapsulated lipid vesicles (ATP-vesicles) while the two wounds on the other side were treated with Regranex. Wound healing time was shorter when ATP-vesicles were used. The most striking finding was that new tissue growth started to appear in less than 1 day when ATP-vesicles were used. The growth continued and covered the wound area within a few days, without the formation of a provisional matrix. Regranex-treated wounds did not have this growth pattern. In wounds treated by ATP-vesicles, histologic studies revealed extremely rich macrophage accumulation, along with active proliferating cell nuclear antigen (PCNA) and positive BrdU staining, indicating in situ macrophage proliferation. Human macrophage culture suggested direct collagen production. These results support an entirely new healing process, which seems to have combined the conventional hemostasis, inflammation, and proliferation phases into a single one, thereby eliminating the lag time usually seen during healing process.
format article
author Jeffrey D Howard
Harshini Sarojini
Rong Wan
Sufan Chien
author_facet Jeffrey D Howard
Harshini Sarojini
Rong Wan
Sufan Chien
author_sort Jeffrey D Howard
title Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_short Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_full Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_fullStr Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_full_unstemmed Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_sort rapid granulation tissue regeneration by intracellular atp delivery--a comparison with regranex.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/2d0cb78c5d9d49ddb90b495a3c6ab888
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AT harshinisarojini rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex
AT rongwan rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex
AT sufanchien rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex
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