Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.

This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic...

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Autores principales:	Jeffrey D Howard, Harshini Sarojini, Rong Wan, Sufan Chien
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d0cb78c5d9d49ddb90b495a3c6ab888
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spelling	oai:doaj.org-article:2d0cb78c5d9d49ddb90b495a3c6ab8882021-11-18T08:27:51ZRapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.1932-620310.1371/journal.pone.0091787https://doaj.org/article/2d0cb78c5d9d49ddb90b495a3c6ab8882014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24637626/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic and four on the normal ear. Two wounds on one side of each ear were treated with Mg-ATP encapsulated lipid vesicles (ATP-vesicles) while the two wounds on the other side were treated with Regranex. Wound healing time was shorter when ATP-vesicles were used. The most striking finding was that new tissue growth started to appear in less than 1 day when ATP-vesicles were used. The growth continued and covered the wound area within a few days, without the formation of a provisional matrix. Regranex-treated wounds did not have this growth pattern. In wounds treated by ATP-vesicles, histologic studies revealed extremely rich macrophage accumulation, along with active proliferating cell nuclear antigen (PCNA) and positive BrdU staining, indicating in situ macrophage proliferation. Human macrophage culture suggested direct collagen production. These results support an entirely new healing process, which seems to have combined the conventional hemostasis, inflammation, and proliferation phases into a single one, thereby eliminating the lag time usually seen during healing process.Jeffrey D HowardHarshini SarojiniRong WanSufan ChienPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91787 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeffrey D Howard Harshini Sarojini Rong Wan Sufan Chien Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
description	This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic and four on the normal ear. Two wounds on one side of each ear were treated with Mg-ATP encapsulated lipid vesicles (ATP-vesicles) while the two wounds on the other side were treated with Regranex. Wound healing time was shorter when ATP-vesicles were used. The most striking finding was that new tissue growth started to appear in less than 1 day when ATP-vesicles were used. The growth continued and covered the wound area within a few days, without the formation of a provisional matrix. Regranex-treated wounds did not have this growth pattern. In wounds treated by ATP-vesicles, histologic studies revealed extremely rich macrophage accumulation, along with active proliferating cell nuclear antigen (PCNA) and positive BrdU staining, indicating in situ macrophage proliferation. Human macrophage culture suggested direct collagen production. These results support an entirely new healing process, which seems to have combined the conventional hemostasis, inflammation, and proliferation phases into a single one, thereby eliminating the lag time usually seen during healing process.
format	article
author	Jeffrey D Howard Harshini Sarojini Rong Wan Sufan Chien
author_facet	Jeffrey D Howard Harshini Sarojini Rong Wan Sufan Chien
author_sort	Jeffrey D Howard
title	Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_short	Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_full	Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_fullStr	Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_full_unstemmed	Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
title_sort	rapid granulation tissue regeneration by intracellular atp delivery--a comparison with regranex.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2d0cb78c5d9d49ddb90b495a3c6ab888
work_keys_str_mv	AT jeffreydhoward rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex AT harshinisarojini rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex AT rongwan rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex AT sufanchien rapidgranulationtissueregenerationbyintracellularatpdeliveryacomparisonwithregranex
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Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.

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