Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases...
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| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/2d1de80c138b4a1ab2404e333d9b965f |
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| Summary: | Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases (PP2Cs) is key to the ABA signal pathway. Due to its excellent effect on endodormancy release, hydrogen cyanamide (HC) treatment is considered an effective measure to study the mechanism of endodormancy release. In this study, RNA-Seq analysis was conducted on endodormant floral buds of pear (<i>Pyrus pyrifolia</i>) with HC treatment, and the HC-induced PP2C gene <i>PpPP2C1</i> was identified. Next, software prediction, expression tests and transient assays revealed that lncRNA <i>PpL-T31511</i>-derived <i>Pp-miRn182</i> targets <i>PpPP2C1</i>. The expression analysis showed that HC treatment upregulated the expression of <i>PpPP2C1</i> and downregulated the expression of <i>PpL-T31511</i> and <i>Pp-miRn182</i>. Moreover, HC treatment inhibited the accumulation of ABA signaling pathway-related genes and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Furthermore, overexpression of <i>Pp-miRn182</i> reduced the inhibitory effect of <i>PpPP2C1</i> on the H<sub>2</sub>O<sub>2</sub> content. In summary, our study suggests that downregulation of <i>PpL-T31511</i>-derived <i>Pp-miRn182</i> promotes HC-induced endodormancy release in pear plants through the PP2C-H<sub>2</sub>O<sub>2</sub> pathway. |
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