Downregulation of lncRNA PpL-T31511 and Pp-miRn182 Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H2O2 Pathway in Pear (Pyrus pyrifolia)

Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases...

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Autores principales:	Liang Li, Jinhang Liu, Qin Liang, Yu Feng, Chao Wang, Shaohua Wu, Yongyu Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pyrus pyrifolia</i> endodormancy hydrogen cyanamide PP2Cs LncRNA <i>PpL-T31511</i> <i>Pp-miRn182</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2d1de80c138b4a1ab2404e333d9b965f
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spelling	oai:doaj.org-article:2d1de80c138b4a1ab2404e333d9b965f2021-11-11T17:16:22ZDownregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)10.3390/ijms2221118421422-00671661-6596https://doaj.org/article/2d1de80c138b4a1ab2404e333d9b965f2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11842https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases (PP2Cs) is key to the ABA signal pathway. Due to its excellent effect on endodormancy release, hydrogen cyanamide (HC) treatment is considered an effective measure to study the mechanism of endodormancy release. In this study, RNA-Seq analysis was conducted on endodormant floral buds of pear (<i>Pyrus pyrifolia</i>) with HC treatment, and the HC-induced PP2C gene <i>PpPP2C1</i> was identified. Next, software prediction, expression tests and transient assays revealed that lncRNA <i>PpL-T31511</i>-derived <i>Pp-miRn182</i> targets <i>PpPP2C1</i>. The expression analysis showed that HC treatment upregulated the expression of <i>PpPP2C1</i> and downregulated the expression of <i>PpL-T31511</i> and <i>Pp-miRn182</i>. Moreover, HC treatment inhibited the accumulation of ABA signaling pathway-related genes and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Furthermore, overexpression of <i>Pp-miRn182</i> reduced the inhibitory effect of <i>PpPP2C1</i> on the H<sub>2</sub>O<sub>2</sub> content. In summary, our study suggests that downregulation of <i>PpL-T31511</i>-derived <i>Pp-miRn182</i> promotes HC-induced endodormancy release in pear plants through the PP2C-H<sub>2</sub>O<sub>2</sub> pathway.Liang LiJinhang LiuQin LiangYu FengChao WangShaohua WuYongyu LiMDPI AGarticle<i>Pyrus pyrifolia</i>endodormancyhydrogen cyanamidePP2CsLncRNA <i>PpL-T31511</i><i>Pp-miRn182</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11842, p 11842 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pyrus pyrifolia</i> endodormancy hydrogen cyanamide PP2Cs LncRNA <i>PpL-T31511</i> <i>Pp-miRn182</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Pyrus pyrifolia</i> endodormancy hydrogen cyanamide PP2Cs LncRNA <i>PpL-T31511</i> <i>Pp-miRn182</i> Biology (General) QH301-705.5 Chemistry QD1-999 Liang Li Jinhang Liu Qin Liang Yu Feng Chao Wang Shaohua Wu Yongyu Li Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
description	Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases (PP2Cs) is key to the ABA signal pathway. Due to its excellent effect on endodormancy release, hydrogen cyanamide (HC) treatment is considered an effective measure to study the mechanism of endodormancy release. In this study, RNA-Seq analysis was conducted on endodormant floral buds of pear (<i>Pyrus pyrifolia</i>) with HC treatment, and the HC-induced PP2C gene <i>PpPP2C1</i> was identified. Next, software prediction, expression tests and transient assays revealed that lncRNA <i>PpL-T31511</i>-derived <i>Pp-miRn182</i> targets <i>PpPP2C1</i>. The expression analysis showed that HC treatment upregulated the expression of <i>PpPP2C1</i> and downregulated the expression of <i>PpL-T31511</i> and <i>Pp-miRn182</i>. Moreover, HC treatment inhibited the accumulation of ABA signaling pathway-related genes and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Furthermore, overexpression of <i>Pp-miRn182</i> reduced the inhibitory effect of <i>PpPP2C1</i> on the H<sub>2</sub>O<sub>2</sub> content. In summary, our study suggests that downregulation of <i>PpL-T31511</i>-derived <i>Pp-miRn182</i> promotes HC-induced endodormancy release in pear plants through the PP2C-H<sub>2</sub>O<sub>2</sub> pathway.
format	article
author	Liang Li Jinhang Liu Qin Liang Yu Feng Chao Wang Shaohua Wu Yongyu Li
author_facet	Liang Li Jinhang Liu Qin Liang Yu Feng Chao Wang Shaohua Wu Yongyu Li
author_sort	Liang Li
title	Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
title_short	Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
title_full	Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
title_fullStr	Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
title_full_unstemmed	Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)
title_sort	downregulation of lncrna <i>ppl-t31511</i> and <i>pp-mirn182</i> promotes hydrogen cyanamide-induced endodormancy release through the pp2c-h<sub>2</sub>o<sub>2</sub> pathway in pear (<i>pyrus pyrifolia</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2d1de80c138b4a1ab2404e333d9b965f
work_keys_str_mv	AT liangli downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai AT jinhangliu downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai AT qinliang downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai AT yufeng downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai AT chaowang downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai AT shaohuawu downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai AT yongyuli downregulationoflncrnaipplt31511iandippmirn182ipromoteshydrogencyanamideinducedendodormancyreleasethroughthepp2chsub2subosub2subpathwayinpearipyruspyrifoliai
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Downregulation of lncRNA <i>PpL-T31511</i> and <i>Pp-miRn182</i> Promotes Hydrogen Cyanamide-Induced Endodormancy Release through the PP2C-H<sub>2</sub>O<sub>2</sub> Pathway in Pear (<i>Pyrus pyrifolia</i>)

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