Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.

<h4>Background</h4>In January 2011, human cases with hemorrhagic manifestations in the hospital staff were reported from a tertiary care hospital in Ahmadabad, India. This paper reports a detailed epidemiological investigation of nosocomial outbreak from the affected area of Ahmadabad, G...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Devendra T Mourya, Pragya D Yadav, Anita M Shete, Yogesh K Gurav, Chandrashekhar G Raut, Ramesh S Jadi, Shailesh D Pawar, Stuart T Nichol, Akhilesh C Mishra
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/2d258236774e4e9c93b9dc46980cf809
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2d258236774e4e9c93b9dc46980cf809
record_format	dspace
spelling	oai:doaj.org-article:2d258236774e4e9c93b9dc46980cf8092021-11-18T09:14:12ZDetection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.1935-27271935-273510.1371/journal.pntd.0001653https://doaj.org/article/2d258236774e4e9c93b9dc46980cf8092012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22616022/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>In January 2011, human cases with hemorrhagic manifestations in the hospital staff were reported from a tertiary care hospital in Ahmadabad, India. This paper reports a detailed epidemiological investigation of nosocomial outbreak from the affected area of Ahmadabad, Gujarat, India.<h4>Principal findings</h4>Samples from 3 suspected cases, 83 contacts, Hyalomma ticks and livestock were screened for Crimean-Congo hemorrhagic fever (CCHF) virus by qRT-PCR of which samples of two medical professionals (case C and E) and the husband of the index case (case D) were positive for CCHFV. The sensitivity and specificity of indigenous developed IgM ELISA to screen CCHFV specific antibodies in human serum was 75.0% and 97.5% respectively as compared to commercial kit. About 17.0% domestic animals from Kolat, Ahmadabad were positive for IgG antibodies while only two cattle and a goat showed positivity by qRT-PCR. Surprisingly, 43.0% domestic animals (Buffalo, cattle, sheep and goat) showed IgG antibodies in the adjoining village Jivanpara but only one of the buffalo was positive for CCHFV. The Hyalomma anatolicum anatolicum ticks were positive in PCR and virus isolation. CCHFV was isolated from the blood sample of case C, E in Vero E-6 cells and Swiss albino mice. In partial nucleocapsid gene phylogeny from CCHFV positive human samples of the years 2010 and 2011, livestock and ticks showed this virus was similar to Tajikistan (strain TAJ/H08966), which belongs in the Asian/middle east genetic lineage IV.<h4>Conclusions</h4>The likely source of CCHFV was identified as virus infected Hyalomma ticks and livestock at the rural village residence of the primary case (case A). In addition, retrospective sample analysis revealed the existence of CCHFV in Gujarat and Rajasthan states before this outbreak. An indigenous developed IgM ELISA kit will be of great use for screening this virus in India.Devendra T MouryaPragya D YadavAnita M SheteYogesh K GuravChandrashekhar G RautRamesh S JadiShailesh D PawarStuart T NicholAkhilesh C MishraPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 6, Iss 5, p e1653 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Devendra T Mourya Pragya D Yadav Anita M Shete Yogesh K Gurav Chandrashekhar G Raut Ramesh S Jadi Shailesh D Pawar Stuart T Nichol Akhilesh C Mishra Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.
description	<h4>Background</h4>In January 2011, human cases with hemorrhagic manifestations in the hospital staff were reported from a tertiary care hospital in Ahmadabad, India. This paper reports a detailed epidemiological investigation of nosocomial outbreak from the affected area of Ahmadabad, Gujarat, India.<h4>Principal findings</h4>Samples from 3 suspected cases, 83 contacts, Hyalomma ticks and livestock were screened for Crimean-Congo hemorrhagic fever (CCHF) virus by qRT-PCR of which samples of two medical professionals (case C and E) and the husband of the index case (case D) were positive for CCHFV. The sensitivity and specificity of indigenous developed IgM ELISA to screen CCHFV specific antibodies in human serum was 75.0% and 97.5% respectively as compared to commercial kit. About 17.0% domestic animals from Kolat, Ahmadabad were positive for IgG antibodies while only two cattle and a goat showed positivity by qRT-PCR. Surprisingly, 43.0% domestic animals (Buffalo, cattle, sheep and goat) showed IgG antibodies in the adjoining village Jivanpara but only one of the buffalo was positive for CCHFV. The Hyalomma anatolicum anatolicum ticks were positive in PCR and virus isolation. CCHFV was isolated from the blood sample of case C, E in Vero E-6 cells and Swiss albino mice. In partial nucleocapsid gene phylogeny from CCHFV positive human samples of the years 2010 and 2011, livestock and ticks showed this virus was similar to Tajikistan (strain TAJ/H08966), which belongs in the Asian/middle east genetic lineage IV.<h4>Conclusions</h4>The likely source of CCHFV was identified as virus infected Hyalomma ticks and livestock at the rural village residence of the primary case (case A). In addition, retrospective sample analysis revealed the existence of CCHFV in Gujarat and Rajasthan states before this outbreak. An indigenous developed IgM ELISA kit will be of great use for screening this virus in India.
format	article
author	Devendra T Mourya Pragya D Yadav Anita M Shete Yogesh K Gurav Chandrashekhar G Raut Ramesh S Jadi Shailesh D Pawar Stuart T Nichol Akhilesh C Mishra
author_facet	Devendra T Mourya Pragya D Yadav Anita M Shete Yogesh K Gurav Chandrashekhar G Raut Ramesh S Jadi Shailesh D Pawar Stuart T Nichol Akhilesh C Mishra
author_sort	Devendra T Mourya
title	Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.
title_short	Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.
title_full	Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.
title_fullStr	Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.
title_full_unstemmed	Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.
title_sort	detection, isolation and confirmation of crimean-congo hemorrhagic fever virus in human, ticks and animals in ahmadabad, india, 2010-2011.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2d258236774e4e9c93b9dc46980cf809
work_keys_str_mv	AT devendratmourya detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT pragyadyadav detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT anitamshete detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT yogeshkgurav detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT chandrashekhargraut detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT rameshsjadi detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT shaileshdpawar detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT stuarttnichol detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011 AT akhileshcmishra detectionisolationandconfirmationofcrimeancongohemorrhagicfevervirusinhumanticksandanimalsinahmadabadindia20102011
_version_	1718420954270400512

Detection, isolation and confirmation of Crimean-Congo hemorrhagic fever virus in human, ticks and animals in Ahmadabad, India, 2010-2011.

Ejemplares similares