Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion

Abstract Drastic changes in gene expression occur after adult female mosquitoes take a blood meal and use the nutrients for egg maturation. A growing body of evidence indicates that microRNAs (miRNAs) contribute to this tightly controlled tissue- and stage-specific gene expression. To investigate th...

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Autores principales:	Xiaonan Fu, George Dimopoulos, Jinsong Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d273692ae144bf2a8d2a0bbf99899a3
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spelling	oai:doaj.org-article:2d273692ae144bf2a8d2a0bbf99899a32021-12-02T12:32:45ZAssociation of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion10.1038/s41598-017-07013-12045-2322https://doaj.org/article/2d273692ae144bf2a8d2a0bbf99899a32017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07013-1https://doaj.org/toc/2045-2322Abstract Drastic changes in gene expression occur after adult female mosquitoes take a blood meal and use the nutrients for egg maturation. A growing body of evidence indicates that microRNAs (miRNAs) contribute to this tightly controlled tissue- and stage-specific gene expression. To investigate the role of miRNAs, we monitored miRNA expression in the mosquito Anopheles gambiae during the 72-h period immediately after blood feeding. We also measured the association of miRNAs with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) to assess the functional status of individual miRNA species. Overall, 173 mature miRNAs were precipitated with Ago1 and Ago2, including 12 new miRNAs, the orthologs of which are found thus far only in other Anopheles species. Ago1 is the predominant carrier of miRNAs in Anopheles gambiae. The abundance and Ago loading of most of the mature miRNAs were relatively stable after blood ingestion. However, miRNAs of the miR-309/286/2944 cluster were considerably upregulated after blood feeding. Injection of the specific antagomir for miR-309 resulted in smaller developing oocytes and ultimately fewer eggs. In addition, the Ago association of some miRNAs was not proportional to their cellular abundance, suggesting that integration of miRNAs into the Ago complexes is regulated by additional mechanisms.Xiaonan FuGeorge DimopoulosJinsong ZhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaonan Fu George Dimopoulos Jinsong Zhu Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
description	Abstract Drastic changes in gene expression occur after adult female mosquitoes take a blood meal and use the nutrients for egg maturation. A growing body of evidence indicates that microRNAs (miRNAs) contribute to this tightly controlled tissue- and stage-specific gene expression. To investigate the role of miRNAs, we monitored miRNA expression in the mosquito Anopheles gambiae during the 72-h period immediately after blood feeding. We also measured the association of miRNAs with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) to assess the functional status of individual miRNA species. Overall, 173 mature miRNAs were precipitated with Ago1 and Ago2, including 12 new miRNAs, the orthologs of which are found thus far only in other Anopheles species. Ago1 is the predominant carrier of miRNAs in Anopheles gambiae. The abundance and Ago loading of most of the mature miRNAs were relatively stable after blood ingestion. However, miRNAs of the miR-309/286/2944 cluster were considerably upregulated after blood feeding. Injection of the specific antagomir for miR-309 resulted in smaller developing oocytes and ultimately fewer eggs. In addition, the Ago association of some miRNAs was not proportional to their cellular abundance, suggesting that integration of miRNAs into the Ago complexes is regulated by additional mechanisms.
format	article
author	Xiaonan Fu George Dimopoulos Jinsong Zhu
author_facet	Xiaonan Fu George Dimopoulos Jinsong Zhu
author_sort	Xiaonan Fu
title	Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
title_short	Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
title_full	Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
title_fullStr	Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
title_full_unstemmed	Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
title_sort	association of micrornas with argonaute proteins in the malaria mosquito anopheles gambiae after blood ingestion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2d273692ae144bf2a8d2a0bbf99899a3
work_keys_str_mv	AT xiaonanfu associationofmicrornaswithargonauteproteinsinthemalariamosquitoanophelesgambiaeafterbloodingestion AT georgedimopoulos associationofmicrornaswithargonauteproteinsinthemalariamosquitoanophelesgambiaeafterbloodingestion AT jinsongzhu associationofmicrornaswithargonauteproteinsinthemalariamosquitoanophelesgambiaeafterbloodingestion
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Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion

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