Comprehensive analysis of bacteriocins in Streptococcus mutans

Abstract Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Ba...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Atsuko Watanabe, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Junzo Hisatsune, Yuichi Oogai, Yoshio Nakano, Masanobu Nakata, Shouichi Miyawaki, Motoyuki Sugai, Hitoshi Komatsuzawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d2bc72b0c464574a17e78b72286db91
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2d2bc72b0c464574a17e78b72286db91
record_format	dspace
spelling	oai:doaj.org-article:2d2bc72b0c464574a17e78b72286db912021-12-02T18:02:50ZComprehensive analysis of bacteriocins in Streptococcus mutans10.1038/s41598-021-92370-12045-2322https://doaj.org/article/2d2bc72b0c464574a17e78b72286db912021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92370-1https://doaj.org/toc/2045-2322Abstract Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on the genome analysis, the distribution of each bacteriocin gene (mutacins I-IV, K8 and Smb) was investigated. We found 17, 5, and 2 strains showing 100% matches with mutacin I, mutacin II and mutacin III, respectively. Five mutacin III-positive strains had 2 mismatches compared to mature mutacin III. In 67 mutacin IV-positive strains, 38 strains showed 100% match with mutacin IV, while 29 strains showed some variations. In 23 mutacin K8- and 32 mutacin Smb-positive strains, all except one mutacin K8-positive strain showed 100% match with the mature peptides. Among 125 strains, 84 (65.1%), 26 (20.2%), and 5 (3.9%) strains were positive for one, two and three bacteriocin genes, respectively. Then, the antibacterial activity against oral streptococci and other oral bacterial species was investigated by using bacteriocin gene single-positive strains. Each bacteriocin gene-positive strain showed a different pattern of antibacterial activity. These results speculate that individual S. mutans strains may affect the bacterial composition of dental plaques.Atsuko WatanabeMiki Kawada-MatsuoMi Nguyen-Tra LeJunzo HisatsuneYuichi OogaiYoshio NakanoMasanobu NakataShouichi MiyawakiMotoyuki SugaiHitoshi KomatsuzawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atsuko Watanabe Miki Kawada-Matsuo Mi Nguyen-Tra Le Junzo Hisatsune Yuichi Oogai Yoshio Nakano Masanobu Nakata Shouichi Miyawaki Motoyuki Sugai Hitoshi Komatsuzawa Comprehensive analysis of bacteriocins in Streptococcus mutans
description	Abstract Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on the genome analysis, the distribution of each bacteriocin gene (mutacins I-IV, K8 and Smb) was investigated. We found 17, 5, and 2 strains showing 100% matches with mutacin I, mutacin II and mutacin III, respectively. Five mutacin III-positive strains had 2 mismatches compared to mature mutacin III. In 67 mutacin IV-positive strains, 38 strains showed 100% match with mutacin IV, while 29 strains showed some variations. In 23 mutacin K8- and 32 mutacin Smb-positive strains, all except one mutacin K8-positive strain showed 100% match with the mature peptides. Among 125 strains, 84 (65.1%), 26 (20.2%), and 5 (3.9%) strains were positive for one, two and three bacteriocin genes, respectively. Then, the antibacterial activity against oral streptococci and other oral bacterial species was investigated by using bacteriocin gene single-positive strains. Each bacteriocin gene-positive strain showed a different pattern of antibacterial activity. These results speculate that individual S. mutans strains may affect the bacterial composition of dental plaques.
format	article
author	Atsuko Watanabe Miki Kawada-Matsuo Mi Nguyen-Tra Le Junzo Hisatsune Yuichi Oogai Yoshio Nakano Masanobu Nakata Shouichi Miyawaki Motoyuki Sugai Hitoshi Komatsuzawa
author_facet	Atsuko Watanabe Miki Kawada-Matsuo Mi Nguyen-Tra Le Junzo Hisatsune Yuichi Oogai Yoshio Nakano Masanobu Nakata Shouichi Miyawaki Motoyuki Sugai Hitoshi Komatsuzawa
author_sort	Atsuko Watanabe
title	Comprehensive analysis of bacteriocins in Streptococcus mutans
title_short	Comprehensive analysis of bacteriocins in Streptococcus mutans
title_full	Comprehensive analysis of bacteriocins in Streptococcus mutans
title_fullStr	Comprehensive analysis of bacteriocins in Streptococcus mutans
title_full_unstemmed	Comprehensive analysis of bacteriocins in Streptococcus mutans
title_sort	comprehensive analysis of bacteriocins in streptococcus mutans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d2bc72b0c464574a17e78b72286db91
work_keys_str_mv	AT atsukowatanabe comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT mikikawadamatsuo comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT minguyentrale comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT junzohisatsune comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT yuichioogai comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT yoshionakano comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT masanobunakata comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT shouichimiyawaki comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT motoyukisugai comprehensiveanalysisofbacteriocinsinstreptococcusmutans AT hitoshikomatsuzawa comprehensiveanalysisofbacteriocinsinstreptococcusmutans
_version_	1718378858065952768

Comprehensive analysis of bacteriocins in Streptococcus mutans

Ejemplares similares