Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus

Abstract Bombyx mori cypovirus (BmCPV), a member of the Reoviridae, specifically infects silkworms and causes extensive economic losses to the sericulture industry. To date, the entry mechanism of BmCPV into cells is unclear. Here we used electron microscopy to study the route of entry of BmCPV into...

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Autores principales:	Fei Chen, Liyuan Zhu, Yiling Zhang, Dhiraj Kumar, Guangli Cao, Xiaolong Hu, Zi Liang, Sulan Kuang, Renyu Xue, Chengliang Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d2fc2a9f714401ca89c0eb7c00f02f1
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spelling	oai:doaj.org-article:2d2fc2a9f714401ca89c0eb7c00f02f12021-12-02T12:32:21ZClathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus10.1038/s41598-018-25677-12045-2322https://doaj.org/article/2d2fc2a9f714401ca89c0eb7c00f02f12018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25677-1https://doaj.org/toc/2045-2322Abstract Bombyx mori cypovirus (BmCPV), a member of the Reoviridae, specifically infects silkworms and causes extensive economic losses to the sericulture industry. To date, the entry mechanism of BmCPV into cells is unclear. Here we used electron microscopy to study the route of entry of BmCPV into cells, and the results demonstrated that the entry of BmCPV into BmN cells was mediated by endocytosis. Blocking the entry pathway with four endocytosis inhibitors, including dansylcadaverine, chlorpromazine, genistein, and PP2, significantly decreased the infectivity of BmCPV. This indicates that BmCPV enters BmN cells via endocytosis, and that clathrin-mediated sorting is the predominant entry method. After the relative expression levels of clathrin heavy chain (clathrin, GenBank accession No. NM_001142971.1) and the adaptor protein complex-1 gamma subunit AP-1 (AP-1, GenBank accession No. JQ824201.1), which are involved in clathrin-mediated endocytosis, were inhibited by RNA interference or abolishing the functions of clathrin and AP-1 with their corresponding antibodies, the infectivity of BmCPV was reduced significantly, which suggests that clathrin-mediated endocytosis contributed to the entry of BmCPV into cells. Our findings suggest that the clathrin-mediated endocytosis pathway is a candidate for the development of therapeutics for silkworm cytoplasmic polyhedrosis.Fei ChenLiyuan ZhuYiling ZhangDhiraj KumarGuangli CaoXiaolong HuZi LiangSulan KuangRenyu XueChengliang GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fei Chen Liyuan Zhu Yiling Zhang Dhiraj Kumar Guangli Cao Xiaolong Hu Zi Liang Sulan Kuang Renyu Xue Chengliang Gong Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus
description	Abstract Bombyx mori cypovirus (BmCPV), a member of the Reoviridae, specifically infects silkworms and causes extensive economic losses to the sericulture industry. To date, the entry mechanism of BmCPV into cells is unclear. Here we used electron microscopy to study the route of entry of BmCPV into cells, and the results demonstrated that the entry of BmCPV into BmN cells was mediated by endocytosis. Blocking the entry pathway with four endocytosis inhibitors, including dansylcadaverine, chlorpromazine, genistein, and PP2, significantly decreased the infectivity of BmCPV. This indicates that BmCPV enters BmN cells via endocytosis, and that clathrin-mediated sorting is the predominant entry method. After the relative expression levels of clathrin heavy chain (clathrin, GenBank accession No. NM_001142971.1) and the adaptor protein complex-1 gamma subunit AP-1 (AP-1, GenBank accession No. JQ824201.1), which are involved in clathrin-mediated endocytosis, were inhibited by RNA interference or abolishing the functions of clathrin and AP-1 with their corresponding antibodies, the infectivity of BmCPV was reduced significantly, which suggests that clathrin-mediated endocytosis contributed to the entry of BmCPV into cells. Our findings suggest that the clathrin-mediated endocytosis pathway is a candidate for the development of therapeutics for silkworm cytoplasmic polyhedrosis.
format	article
author	Fei Chen Liyuan Zhu Yiling Zhang Dhiraj Kumar Guangli Cao Xiaolong Hu Zi Liang Sulan Kuang Renyu Xue Chengliang Gong
author_facet	Fei Chen Liyuan Zhu Yiling Zhang Dhiraj Kumar Guangli Cao Xiaolong Hu Zi Liang Sulan Kuang Renyu Xue Chengliang Gong
author_sort	Fei Chen
title	Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus
title_short	Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus
title_full	Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus
title_fullStr	Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus
title_full_unstemmed	Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus
title_sort	clathrin-mediated endocytosis is a candidate entry sorting mechanism for bombyx mori cypovirus
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/2d2fc2a9f714401ca89c0eb7c00f02f1
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Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus

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