Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum

Abstract High biomasses of the marine dinoflagellate Lepidodinium chlorophorum cause green seawater discolorations along Southern Brittany (NE Atlantic, France). The viscosity associated to these phenomena has been related to problems in oyster cultivation. The harmful effect of L. chlorophorum migh...

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Autores principales:	Pauline Roux, Raffaele Siano, Karine Collin, Gwenael Bilien, Corinne Sinquin, Laetitia Marchand, Agata Zykwinska, Christine Delbarre-Ladrat, Mathilde Schapira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d31dc84fa834db3a2cac870a3f8329c
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spelling	oai:doaj.org-article:2d31dc84fa834db3a2cac870a3f8329c2021-12-02T13:34:57ZBacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum10.1038/s41598-021-84253-22045-2322https://doaj.org/article/2d31dc84fa834db3a2cac870a3f8329c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84253-2https://doaj.org/toc/2045-2322Abstract High biomasses of the marine dinoflagellate Lepidodinium chlorophorum cause green seawater discolorations along Southern Brittany (NE Atlantic, France). The viscosity associated to these phenomena has been related to problems in oyster cultivation. The harmful effect of L. chlorophorum might originate from the secretion of Extracellular Polymeric Substances (EPS). To understand whether the EPS are produced by L. chlorophorum or its associated bacteria, or if they are a product of their interaction, batch cultures were performed under non-axenic and pseudo-axenic conditions for three strains. Maximum dinoflagellate cell abundances were observed in pseudo-axenic cultures. The non-sinking fraction of polymers (Soluble Extracellular Polymers, SEP), mainly composed of proteins and the exopolysaccharide sulphated galactan, slightly increased in pseudo-axenic cultures. The amount of Transparent Exopolymer Particles (TEP) per cell increased under non-axenic conditions. Despite the high concentrations of Particulate Organic Carbon (POC) measured, viscosity did not vary. These results suggest that the L. chlorophorum-bacteria interaction could have a detrimental consequence on the dinoflagellate, translating in a negative effect on L. chlorophorum growth, as well as EPS overproduction by the dinoflagellate, at concentrations that should not affect seawater viscosity.Pauline RouxRaffaele SianoKarine CollinGwenael BilienCorinne SinquinLaetitia MarchandAgata ZykwinskaChristine Delbarre-LadratMathilde SchapiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pauline Roux Raffaele Siano Karine Collin Gwenael Bilien Corinne Sinquin Laetitia Marchand Agata Zykwinska Christine Delbarre-Ladrat Mathilde Schapira Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
description	Abstract High biomasses of the marine dinoflagellate Lepidodinium chlorophorum cause green seawater discolorations along Southern Brittany (NE Atlantic, France). The viscosity associated to these phenomena has been related to problems in oyster cultivation. The harmful effect of L. chlorophorum might originate from the secretion of Extracellular Polymeric Substances (EPS). To understand whether the EPS are produced by L. chlorophorum or its associated bacteria, or if they are a product of their interaction, batch cultures were performed under non-axenic and pseudo-axenic conditions for three strains. Maximum dinoflagellate cell abundances were observed in pseudo-axenic cultures. The non-sinking fraction of polymers (Soluble Extracellular Polymers, SEP), mainly composed of proteins and the exopolysaccharide sulphated galactan, slightly increased in pseudo-axenic cultures. The amount of Transparent Exopolymer Particles (TEP) per cell increased under non-axenic conditions. Despite the high concentrations of Particulate Organic Carbon (POC) measured, viscosity did not vary. These results suggest that the L. chlorophorum-bacteria interaction could have a detrimental consequence on the dinoflagellate, translating in a negative effect on L. chlorophorum growth, as well as EPS overproduction by the dinoflagellate, at concentrations that should not affect seawater viscosity.
format	article
author	Pauline Roux Raffaele Siano Karine Collin Gwenael Bilien Corinne Sinquin Laetitia Marchand Agata Zykwinska Christine Delbarre-Ladrat Mathilde Schapira
author_facet	Pauline Roux Raffaele Siano Karine Collin Gwenael Bilien Corinne Sinquin Laetitia Marchand Agata Zykwinska Christine Delbarre-Ladrat Mathilde Schapira
author_sort	Pauline Roux
title	Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
title_short	Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
title_full	Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
title_fullStr	Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
title_full_unstemmed	Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum
title_sort	bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate lepidodinium chlorophorum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d31dc84fa834db3a2cac870a3f8329c
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Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum

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