Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.

Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We ass...

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Autores principales:	Verónica Ponce de León, Anne-Marie Mérillat, Laurent Tesson, Ignacio Anegón, Edith Hummler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d32fd8d3b0f4b059d6202bbf313b382
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spelling	oai:doaj.org-article:2d32fd8d3b0f4b059d6202bbf313b3822021-11-18T08:33:01ZGeneration of TALEN-mediated GRdim knock-in rats by homologous recombination.1932-620310.1371/journal.pone.0088146https://doaj.org/article/2d32fd8d3b0f4b059d6202bbf313b3822014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24523878/?tool=EBIhttps://doaj.org/toc/1932-6203Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.Verónica Ponce de LeónAnne-Marie MérillatLaurent TessonIgnacio AnegónEdith HummlerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88146 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Verónica Ponce de León Anne-Marie Mérillat Laurent Tesson Ignacio Anegón Edith Hummler Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
description	Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.
format	article
author	Verónica Ponce de León Anne-Marie Mérillat Laurent Tesson Ignacio Anegón Edith Hummler
author_facet	Verónica Ponce de León Anne-Marie Mérillat Laurent Tesson Ignacio Anegón Edith Hummler
author_sort	Verónica Ponce de León
title	Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
title_short	Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
title_full	Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
title_fullStr	Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
title_full_unstemmed	Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
title_sort	generation of talen-mediated grdim knock-in rats by homologous recombination.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2d32fd8d3b0f4b059d6202bbf313b382
work_keys_str_mv	AT veronicaponcedeleon generationoftalenmediatedgrdimknockinratsbyhomologousrecombination AT annemariemerillat generationoftalenmediatedgrdimknockinratsbyhomologousrecombination AT laurenttesson generationoftalenmediatedgrdimknockinratsbyhomologousrecombination AT ignacioanegon generationoftalenmediatedgrdimknockinratsbyhomologousrecombination AT edithhummler generationoftalenmediatedgrdimknockinratsbyhomologousrecombination
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Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.

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