Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Heba M Salem,1 Elshaimaa Ismael,2 Mohamed Shaalan3 1Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 2Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 3Department of Pathology,...

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Autores principales:	Salem HM, Ismael E, Shaalan M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	broiler chickens clostridium perfringens fcr histopathology necrotic enteritis residues silver nanoparticles. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2d38516323f94ed587003414f937161b
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spelling	oai:doaj.org-article:2d38516323f94ed587003414f937161b2021-12-02T19:18:50ZEvaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens1178-2013https://doaj.org/article/2d38516323f94ed587003414f937161b2021-10-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-the-effects-of-silver-nanoparticles-against-experimental-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Heba M Salem,1 Elshaimaa Ismael,2 Mohamed Shaalan3 1Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 2Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 3Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, EgyptCorrespondence: Mohamed ShaalanDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, EgyptTel +20 1151854844Email mohamedibrahim@cu.edu.egBackground: Clostridium perfringens-associated necrotic enteritis (NE) is a serious problem affecting broiler production. A major global challenge is to reduce the use of antibiotics in poultry industry due to their negative impacts on public health. One alternative is to use nanoparticles (NPs) to overcome bacterial resistance to antibiotics. Silver nanoparticles (Ag NPs) showed strong antimicrobial activity.Methods: A total of 120 Cobb broiler chicks (1-day old) were obtained for this study and were divided into 4 equal groups at age of 14 days (30 birds each); each group was subdivided into 3 equal replicates (10 birds each). The groups were designated as follows: G1, infected; G2, infected and treated with Ag NPs; G3, treated with Ag NPs; and G4, negative control. Birds were infected with 4× 108 colony forming unit (CFU)/mL/bird C. perfringens type A for 2 successive days. In the treated groups, Ag NPs (mean diameter 15 nm; total dose 150 μg/bird) were administered via crop gavage. During the observation period (5 weeks), bird performance and immune organ indexes were recorded. Serum samples were collected for immunological evaluation, and tissue samples were collected for histopathology and estimation of Ag NPs residues.Results: Treatment with Ag NPs reduced the colonization of C. perfringens in the intestine and ceca, decreased the severity of clinical signs and reduced mortalities in comparison with infected non-treated group. Ag NPs treatment alleviated pathological lesions in the intestine and liver, but their residues were found in the muscles.Conclusion: Ag NPs have a positive impact on gut health integrity while having no impact on immune organs. Ag NPs have some residues in muscles; therefore, further studies are needed on the concentration and size of Ag NPs, the route of administration, and withdrawal time to ensure the safety of chicken meat for human consumption.Keywords: broiler chickens, Clostridium perfringens, FCR, histopathology, necrotic enteritis, residues, silver nanoparticlesSalem HMIsmael EShaalan MDove Medical Pressarticlebroiler chickensclostridium perfringensfcrhistopathologynecrotic enteritisresiduessilver nanoparticles.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 6783-6796 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	broiler chickens clostridium perfringens fcr histopathology necrotic enteritis residues silver nanoparticles. Medicine (General) R5-920
spellingShingle	broiler chickens clostridium perfringens fcr histopathology necrotic enteritis residues silver nanoparticles. Medicine (General) R5-920 Salem HM Ismael E Shaalan M Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens
description	Heba M Salem,1 Elshaimaa Ismael,2 Mohamed Shaalan3 1Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 2Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 3Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, EgyptCorrespondence: Mohamed ShaalanDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, EgyptTel +20 1151854844Email mohamedibrahim@cu.edu.egBackground: Clostridium perfringens-associated necrotic enteritis (NE) is a serious problem affecting broiler production. A major global challenge is to reduce the use of antibiotics in poultry industry due to their negative impacts on public health. One alternative is to use nanoparticles (NPs) to overcome bacterial resistance to antibiotics. Silver nanoparticles (Ag NPs) showed strong antimicrobial activity.Methods: A total of 120 Cobb broiler chicks (1-day old) were obtained for this study and were divided into 4 equal groups at age of 14 days (30 birds each); each group was subdivided into 3 equal replicates (10 birds each). The groups were designated as follows: G1, infected; G2, infected and treated with Ag NPs; G3, treated with Ag NPs; and G4, negative control. Birds were infected with 4× 108 colony forming unit (CFU)/mL/bird C. perfringens type A for 2 successive days. In the treated groups, Ag NPs (mean diameter 15 nm; total dose 150 μg/bird) were administered via crop gavage. During the observation period (5 weeks), bird performance and immune organ indexes were recorded. Serum samples were collected for immunological evaluation, and tissue samples were collected for histopathology and estimation of Ag NPs residues.Results: Treatment with Ag NPs reduced the colonization of C. perfringens in the intestine and ceca, decreased the severity of clinical signs and reduced mortalities in comparison with infected non-treated group. Ag NPs treatment alleviated pathological lesions in the intestine and liver, but their residues were found in the muscles.Conclusion: Ag NPs have a positive impact on gut health integrity while having no impact on immune organs. Ag NPs have some residues in muscles; therefore, further studies are needed on the concentration and size of Ag NPs, the route of administration, and withdrawal time to ensure the safety of chicken meat for human consumption.Keywords: broiler chickens, Clostridium perfringens, FCR, histopathology, necrotic enteritis, residues, silver nanoparticles
format	article
author	Salem HM Ismael E Shaalan M
author_facet	Salem HM Ismael E Shaalan M
author_sort	Salem HM
title	Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens
title_short	Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens
title_full	Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens
title_fullStr	Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens
title_full_unstemmed	Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens
title_sort	evaluation of the effects of silver nanoparticles against experimentally induced necrotic enteritis in broiler chickens
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/2d38516323f94ed587003414f937161b
work_keys_str_mv	AT salemhm evaluationoftheeffectsofsilvernanoparticlesagainstexperimentallyinducednecroticenteritisinbroilerchickens AT ismaele evaluationoftheeffectsofsilvernanoparticlesagainstexperimentallyinducednecroticenteritisinbroilerchickens AT shaalanm evaluationoftheeffectsofsilvernanoparticlesagainstexperimentallyinducednecroticenteritisinbroilerchickens
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Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

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