Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats

Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual...

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Autores principales:	Bjelica Jelena, Jovanović Ivan B., Ilić Vesna, Valčić Olivera, Kirovski Danijela, Gvozdić Dragan, Milanović Svetlana
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	glutathione peroxidase igm macrophage adherence macrophage peroxide production rats selenium thyroid hormones Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/2d3898b6cb864e669bb26e9095d8a275
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spelling	oai:doaj.org-article:2d3898b6cb864e669bb26e9095d8a2752021-11-17T21:27:51ZEffects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats1820-744810.1515/acve-2017-0028https://doaj.org/article/2d3898b6cb864e669bb26e9095d8a2752017-09-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0028https://doaj.org/toc/1820-7448Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU- group; selenium adequate, PTU+ group; selenium defi cient, PTU- group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T4), triiodothyronine (T3), and thyroid stimulating hormone (TSH) were determined. The animals having “intermediate” concentrations of T3 (1.56-1.69 nmol/L) and T4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T3≤1.55 nmol/L; T4≤40 nmol/L), while PTU- groups included euthyroid rats (T3≥1.70 nmol/L; T4≥50 nmol/L). Both groups of selenium deficient rats had, when compared to the control group, a significantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium deficiency nor PTU influenced the adherence of peritoneal macrophages. Selenium deficiency significantly decreased the peroxide synthesis in macrophages and significantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium deficiency had no influence on these parameters.Bjelica JelenaJovanović Ivan B.Ilić VesnaValčić OliveraKirovski DanijelaGvozdić DraganMilanović SvetlanaSciendoarticleglutathione peroxidaseigmmacrophage adherencemacrophage peroxide productionratsseleniumthyroid hormonesVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 3, Pp 340-355 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	glutathione peroxidase igm macrophage adherence macrophage peroxide production rats selenium thyroid hormones Veterinary medicine SF600-1100
spellingShingle	glutathione peroxidase igm macrophage adherence macrophage peroxide production rats selenium thyroid hormones Veterinary medicine SF600-1100 Bjelica Jelena Jovanović Ivan B. Ilić Vesna Valčić Olivera Kirovski Danijela Gvozdić Dragan Milanović Svetlana Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
description	Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU- group; selenium adequate, PTU+ group; selenium defi cient, PTU- group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T4), triiodothyronine (T3), and thyroid stimulating hormone (TSH) were determined. The animals having “intermediate” concentrations of T3 (1.56-1.69 nmol/L) and T4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T3≤1.55 nmol/L; T4≤40 nmol/L), while PTU- groups included euthyroid rats (T3≥1.70 nmol/L; T4≥50 nmol/L). Both groups of selenium deficient rats had, when compared to the control group, a significantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium deficiency nor PTU influenced the adherence of peritoneal macrophages. Selenium deficiency significantly decreased the peroxide synthesis in macrophages and significantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium deficiency had no influence on these parameters.
format	article
author	Bjelica Jelena Jovanović Ivan B. Ilić Vesna Valčić Olivera Kirovski Danijela Gvozdić Dragan Milanović Svetlana
author_facet	Bjelica Jelena Jovanović Ivan B. Ilić Vesna Valčić Olivera Kirovski Danijela Gvozdić Dragan Milanović Svetlana
author_sort	Bjelica Jelena
title	Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
title_short	Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
title_full	Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
title_fullStr	Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
title_full_unstemmed	Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats
title_sort	effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural igm antibodies in juvenile rats
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/2d3898b6cb864e669bb26e9095d8a275
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Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats

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