A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo.
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Nature Portfolio
2021
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oai:doaj.org-article:2d42390e6b6242feac70593cf3d675b12021-12-02T17:52:08ZA versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses10.1038/s41467-021-23779-52041-1723https://doaj.org/article/2d42390e6b6242feac70593cf3d675b12021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23779-5https://doaj.org/toc/2041-1723Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo.Alberto A. AmarillaJulian D. J. SngRhys ParryJoshua M. DeerainJames R. PotterYin Xiang SetohDaniel J. RawleThuy T. LeNaphak ModhiranXiaohui WangNias Y. G. PengFrancisco J. TorresAlyssa PykeJessica J. HarrisonMorgan E. FreneyBenjamin LiangChristopher L. D. McMillanStacey T. M. CheungDarwin J. Da Costa GuevaraJoshua M. HardyMark BettingtonDavid A. MullerFasséli CoulibalyFrederick MooreRoy A. HallPaul R. YoungJason M. MackenzieJody Hobson-PetersAndreas SuhrbierDaniel WattersonAlexander A. KhromykhNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021) |
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DOAJ |
| collection |
DOAJ |
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EN |
| topic |
Science Q |
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Science Q Alberto A. Amarilla Julian D. J. Sng Rhys Parry Joshua M. Deerain James R. Potter Yin Xiang Setoh Daniel J. Rawle Thuy T. Le Naphak Modhiran Xiaohui Wang Nias Y. G. Peng Francisco J. Torres Alyssa Pyke Jessica J. Harrison Morgan E. Freney Benjamin Liang Christopher L. D. McMillan Stacey T. M. Cheung Darwin J. Da Costa Guevara Joshua M. Hardy Mark Bettington David A. Muller Fasséli Coulibaly Frederick Moore Roy A. Hall Paul R. Young Jason M. Mackenzie Jody Hobson-Peters Andreas Suhrbier Daniel Watterson Alexander A. Khromykh A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |
| description |
Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo. |
| format |
article |
| author |
Alberto A. Amarilla Julian D. J. Sng Rhys Parry Joshua M. Deerain James R. Potter Yin Xiang Setoh Daniel J. Rawle Thuy T. Le Naphak Modhiran Xiaohui Wang Nias Y. G. Peng Francisco J. Torres Alyssa Pyke Jessica J. Harrison Morgan E. Freney Benjamin Liang Christopher L. D. McMillan Stacey T. M. Cheung Darwin J. Da Costa Guevara Joshua M. Hardy Mark Bettington David A. Muller Fasséli Coulibaly Frederick Moore Roy A. Hall Paul R. Young Jason M. Mackenzie Jody Hobson-Peters Andreas Suhrbier Daniel Watterson Alexander A. Khromykh |
| author_facet |
Alberto A. Amarilla Julian D. J. Sng Rhys Parry Joshua M. Deerain James R. Potter Yin Xiang Setoh Daniel J. Rawle Thuy T. Le Naphak Modhiran Xiaohui Wang Nias Y. G. Peng Francisco J. Torres Alyssa Pyke Jessica J. Harrison Morgan E. Freney Benjamin Liang Christopher L. D. McMillan Stacey T. M. Cheung Darwin J. Da Costa Guevara Joshua M. Hardy Mark Bettington David A. Muller Fasséli Coulibaly Frederick Moore Roy A. Hall Paul R. Young Jason M. Mackenzie Jody Hobson-Peters Andreas Suhrbier Daniel Watterson Alexander A. Khromykh |
| author_sort |
Alberto A. Amarilla |
| title |
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |
| title_short |
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |
| title_full |
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |
| title_fullStr |
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |
| title_full_unstemmed |
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |
| title_sort |
versatile reverse genetics platform for sars-cov-2 and other positive-strand rna viruses |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/2d42390e6b6242feac70593cf3d675b1 |
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