TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat

TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat

Fusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (<i>Triticum aestivum</i>) and barley. Both diseases are predominately caused by the pathogenic fungi, <i>Fusarium graminearum</i> and <i>Rhizoctonia cerealis...

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Autores principales: Feilong Guo, Tianci Wu, Gangbiao Xu, Haijun Qi, Xiuliang Zhu, Zengyan Zhang
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
bread wheat (<i>Triticum aestivum</i>)
chitin-induced immunity
<i>Fusarium graminearum</i>
<i>Rhizoctonia cereal</i>
wall-associated kinase (WAK)
Biology (General)
QH301-705.5
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/2d42f2e9b07b44c3ab9050878670dade
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spelling oai:doaj.org-article:2d42f2e9b07b44c3ab9050878670dade2021-11-11T16:57:26ZTaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat10.3390/ijms2221114931422-00671661-6596https://doaj.org/article/2d42f2e9b07b44c3ab9050878670dade2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11493https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Fusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (<i>Triticum aestivum</i>) and barley. Both diseases are predominately caused by the pathogenic fungi, <i>Fusarium graminearum</i> and <i>Rhizoctonia cerealis</i>. The roles of the wheat-wall-associated kinases (WAKs) in defense against both <i>F. graminearum</i> and <i>R. cerealis</i> have remained largely unknown. This research reports the identification of <i>TaWAK2A-800</i>, a wheat <i>WAK</i>-coding gene located on chromosome 2A, and its functional roles in wheat resistance responses to FHB and sharp eyespot. <i>TaWAK2A-800</i> transcript abundance was elevated by the early infection of <i>R. cerealis</i> and <i>F. graminearum</i>, or treatment with exogenous chitin. The gene transcript seemed to correspond to the resistance of wheat. Further functional analyses showed that silencing <i>TaWAK2A-800</i> compromised the resistance of wheat to both FHB (<i>F. graminearum</i>) and sharp eyespot (<i>R. cerealis</i>). Moreover, the silencing reduced the expression levels of six defense-related genes, including the chitin-triggering immune pathway-marker genes, <i>TaCERK1</i>, <i>TaRLCK1B</i>, and <i>TaMPK3</i>. Summarily, <i>TaWAK2A-800</i> participates positively in the resistance responses to both <i>F. graminearum</i> and <i>R. cerealis</i>, possibly through a chitin-induced pathway in wheat. <i>TaWAK2A-800</i> will be useful for breeding wheat varieties with resistance to both FHB and sharp eyespot.Feilong GuoTianci WuGangbiao XuHaijun QiXiuliang ZhuZengyan ZhangMDPI AGarticlebread wheat (<i>Triticum aestivum</i>)chitin-induced immunity<i>Fusarium graminearum</i><i>Rhizoctonia cereal</i>wall-associated kinase (WAK)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11493, p 11493 (2021)
institution DOAJ
collection DOAJ
language EN
topic bread wheat (<i>Triticum aestivum</i>)
chitin-induced immunity
<i>Fusarium graminearum</i>
<i>Rhizoctonia cereal</i>
wall-associated kinase (WAK)
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle bread wheat (<i>Triticum aestivum</i>)
chitin-induced immunity
<i>Fusarium graminearum</i>
<i>Rhizoctonia cereal</i>
wall-associated kinase (WAK)
Biology (General)
QH301-705.5
Chemistry
QD1-999
Feilong Guo
Tianci Wu
Gangbiao Xu
Haijun Qi
Xiuliang Zhu
Zengyan Zhang
TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
description Fusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (<i>Triticum aestivum</i>) and barley. Both diseases are predominately caused by the pathogenic fungi, <i>Fusarium graminearum</i> and <i>Rhizoctonia cerealis</i>. The roles of the wheat-wall-associated kinases (WAKs) in defense against both <i>F. graminearum</i> and <i>R. cerealis</i> have remained largely unknown. This research reports the identification of <i>TaWAK2A-800</i>, a wheat <i>WAK</i>-coding gene located on chromosome 2A, and its functional roles in wheat resistance responses to FHB and sharp eyespot. <i>TaWAK2A-800</i> transcript abundance was elevated by the early infection of <i>R. cerealis</i> and <i>F. graminearum</i>, or treatment with exogenous chitin. The gene transcript seemed to correspond to the resistance of wheat. Further functional analyses showed that silencing <i>TaWAK2A-800</i> compromised the resistance of wheat to both FHB (<i>F. graminearum</i>) and sharp eyespot (<i>R. cerealis</i>). Moreover, the silencing reduced the expression levels of six defense-related genes, including the chitin-triggering immune pathway-marker genes, <i>TaCERK1</i>, <i>TaRLCK1B</i>, and <i>TaMPK3</i>. Summarily, <i>TaWAK2A-800</i> participates positively in the resistance responses to both <i>F. graminearum</i> and <i>R. cerealis</i>, possibly through a chitin-induced pathway in wheat. <i>TaWAK2A-800</i> will be useful for breeding wheat varieties with resistance to both FHB and sharp eyespot.
format article
author Feilong Guo
Tianci Wu
Gangbiao Xu
Haijun Qi
Xiuliang Zhu
Zengyan Zhang
author_facet Feilong Guo
Tianci Wu
Gangbiao Xu
Haijun Qi
Xiuliang Zhu
Zengyan Zhang
author_sort Feilong Guo
title TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
title_short TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
title_full TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
title_fullStr TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
title_full_unstemmed TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat
title_sort tawak2a-800, a wall-associated kinase, participates positively in resistance to fusarium head blight and sharp eyespot in wheat
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/2d42f2e9b07b44c3ab9050878670dade
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