Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.

Glucocorticoid (GC) induced osteoporosis (GIO) is caused by the long-term use of GC for treatment of autoimmune and inflammatory diseases. The GC related disruption of bone marrow microcirculation and increased adipogenesis contribute to GIO development. However, neither currently available anti-ost...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liao Cui, Ting Li, Yuyu Liu, Le Zhou, Pinghua Li, Bilian Xu, Lianfang Huang, Yan Chen, Yanzhi Liu, Xiaoyan Tian, Webster S S Jee, Tie Wu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d56af49c6ad42a7b80a952a9dec5e26
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2d56af49c6ad42a7b80a952a9dec5e26
record_format	dspace
spelling	oai:doaj.org-article:2d56af49c6ad42a7b80a952a9dec5e262021-11-18T07:23:00ZSalvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.1932-620310.1371/journal.pone.0034647https://doaj.org/article/2d56af49c6ad42a7b80a952a9dec5e262012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22493705/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Glucocorticoid (GC) induced osteoporosis (GIO) is caused by the long-term use of GC for treatment of autoimmune and inflammatory diseases. The GC related disruption of bone marrow microcirculation and increased adipogenesis contribute to GIO development. However, neither currently available anti-osteoporosis agent is completely addressed to microcirculation and bone marrow adipogenesis. Salvianolic acid B (Sal B) is a polyphenolic compound from a Chinese herbal medicine, Salvia miltiorrhiza Bunge. The aim of this study was to determine the effects of Sal B on osteoblast bone formation, angiogenesis and adipogenesis-associated GIO by performing marrow adipogenesis and microcirculation dilation and bone histomorphometry analyses. (1) In vivo study: Bone loss in GC treated rats was confirmed by significantly decreased BMD, bone strength, cancellous bone mass and architecture, osteoblast distribution, bone formation, marrow microvessel density and diameter along with down-regulation of marrow BMPs expression and increased adipogenesis. Daily treatment with Sal B (40 mg/kg/d) for 12 weeks in GC male rats prevented GC-induced cancellous bone loss and increased adipogenesis while increasing cancellous bone formation rate with improved local microcirculation by capillary dilation. Treatment with Sal B at a higher dose (80 mg/kg/d) not only prevented GC-induced osteopenia, but also increased cancellous bone mass and thickness, associated with increase of marrow BMPs expression, inhibited adipogenesis and further increased microvessel diameters. (2) In vitro study: In concentration from 10(-6) mol/L to 10(-7) mol/L, Sal B stimulated bone marrow stromal cell (MSC) differentiation to osteoblast and increased osteoblast activities, decreased GC associated adipogenic differentiation by down-regulation of PPARγ mRNA expression, increased Runx2 mRNA expression without osteoblast inducement, and, furthermore, Sal B decreased Dickkopf-1 and increased β-catenin mRNA expression with or without adipocyte inducement in MSC. We conclude that Sal B prevented bone loss in GC-treated rats through stimulation of osteogenesis, bone marrow angiogenesis and inhibition of adipogenesis.Liao CuiTing LiYuyu LiuLe ZhouPinghua LiBilian XuLianfang HuangYan ChenYanzhi LiuXiaoyan TianWebster S S JeeTie WuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34647 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liao Cui Ting Li Yuyu Liu Le Zhou Pinghua Li Bilian Xu Lianfang Huang Yan Chen Yanzhi Liu Xiaoyan Tian Webster S S Jee Tie Wu Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
description	Glucocorticoid (GC) induced osteoporosis (GIO) is caused by the long-term use of GC for treatment of autoimmune and inflammatory diseases. The GC related disruption of bone marrow microcirculation and increased adipogenesis contribute to GIO development. However, neither currently available anti-osteoporosis agent is completely addressed to microcirculation and bone marrow adipogenesis. Salvianolic acid B (Sal B) is a polyphenolic compound from a Chinese herbal medicine, Salvia miltiorrhiza Bunge. The aim of this study was to determine the effects of Sal B on osteoblast bone formation, angiogenesis and adipogenesis-associated GIO by performing marrow adipogenesis and microcirculation dilation and bone histomorphometry analyses. (1) In vivo study: Bone loss in GC treated rats was confirmed by significantly decreased BMD, bone strength, cancellous bone mass and architecture, osteoblast distribution, bone formation, marrow microvessel density and diameter along with down-regulation of marrow BMPs expression and increased adipogenesis. Daily treatment with Sal B (40 mg/kg/d) for 12 weeks in GC male rats prevented GC-induced cancellous bone loss and increased adipogenesis while increasing cancellous bone formation rate with improved local microcirculation by capillary dilation. Treatment with Sal B at a higher dose (80 mg/kg/d) not only prevented GC-induced osteopenia, but also increased cancellous bone mass and thickness, associated with increase of marrow BMPs expression, inhibited adipogenesis and further increased microvessel diameters. (2) In vitro study: In concentration from 10(-6) mol/L to 10(-7) mol/L, Sal B stimulated bone marrow stromal cell (MSC) differentiation to osteoblast and increased osteoblast activities, decreased GC associated adipogenic differentiation by down-regulation of PPARγ mRNA expression, increased Runx2 mRNA expression without osteoblast inducement, and, furthermore, Sal B decreased Dickkopf-1 and increased β-catenin mRNA expression with or without adipocyte inducement in MSC. We conclude that Sal B prevented bone loss in GC-treated rats through stimulation of osteogenesis, bone marrow angiogenesis and inhibition of adipogenesis.
format	article
author	Liao Cui Ting Li Yuyu Liu Le Zhou Pinghua Li Bilian Xu Lianfang Huang Yan Chen Yanzhi Liu Xiaoyan Tian Webster S S Jee Tie Wu
author_facet	Liao Cui Ting Li Yuyu Liu Le Zhou Pinghua Li Bilian Xu Lianfang Huang Yan Chen Yanzhi Liu Xiaoyan Tian Webster S S Jee Tie Wu
author_sort	Liao Cui
title	Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
title_short	Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
title_full	Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
title_fullStr	Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
title_full_unstemmed	Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
title_sort	salvianolic acid b prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2d56af49c6ad42a7b80a952a9dec5e26
work_keys_str_mv	AT liaocui salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT tingli salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT yuyuliu salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT lezhou salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT pinghuali salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT bilianxu salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT lianfanghuang salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT yanchen salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT yanzhiliu salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT xiaoyantian salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT websterssjee salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis AT tiewu salvianolicacidbpreventsbonelossinprednisonetreatedratsthroughstimulationofosteogenesisandbonemarrowangiogenesis
_version_	1718423525378752512

Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis.

Ejemplares similares