The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation

Abstract Foot electrical stimulation (FES) has been considered as a classic stressor that can disturb homeostasis. Acute anemia was observed in the model induced by FES. The aim of this study was to explore the role of inflammatory cytokines underlying the acute anemia and gastrointestinal (GI) muco...

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Autores principales:	Fangcheng Fan, Yangwen Ai, Ting Sun, Shuran Li, Hua Liu, Xiaojie Shi, Ziqian Zhang, Qingshan Liu, Yong Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d64b4bf6350415888ff08e0a4af53e8
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spelling	oai:doaj.org-article:2d64b4bf6350415888ff08e0a4af53e82021-12-02T14:06:55ZThe role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation10.1038/s41598-021-82604-72045-2322https://doaj.org/article/2d64b4bf6350415888ff08e0a4af53e82021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82604-7https://doaj.org/toc/2045-2322Abstract Foot electrical stimulation (FES) has been considered as a classic stressor that can disturb homeostasis. Acute anemia was observed in the model induced by FES. The aim of this study was to explore the role of inflammatory cytokines underlying the acute anemia and gastrointestinal (GI) mucosal injury in the FES. Twenty-four male Kunming mice (20 ± 2 g) were randomly divided into control group and experimental group. The mice were placed in a footshock chamber that can generate 0.5 mA electrical impulse periodically for 0.5 h. After the process, red blood cell count, hemoglobin concentration and hematocrit, the levels of corticotropin releasing hormone (CRH) in serum and hypothalamus, and adrenocorticotropic hormone (ACTH) in serum and pituitary were detected separately. In addition, we investigated the expressions of inflammatory cytokines (IL-1, IL-6, TNF-α, iNOS, and IL-10) in the hypothalamus and duodenum by Polymerase Chain Reaction (PCR). Results showed that this FES model induced anemia, increased CRH and ACTH activity in the serum after the FES. Moreover, the expressions of IL-1β, IL-6, TNF-α, and iNOS were significantly increased following the process, while IL-10 was not activated. These findings suggest that anemia, the inflammatory cytokines in the hypothalamus and duodenum of the mice in the model induced by FES is closely related to GI mucosal injury/bleeding. Taken together, these results underscore the importance of anemia, GI mucosal injury/bleeding and stress, future studies would be needed to translate these findings into the benefit of affected patients.Fangcheng FanYangwen AiTing SunShuran LiHua LiuXiaojie ShiZiqian ZhangQingshan LiuYong ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fangcheng Fan Yangwen Ai Ting Sun Shuran Li Hua Liu Xiaojie Shi Ziqian Zhang Qingshan Liu Yong Cheng The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
description	Abstract Foot electrical stimulation (FES) has been considered as a classic stressor that can disturb homeostasis. Acute anemia was observed in the model induced by FES. The aim of this study was to explore the role of inflammatory cytokines underlying the acute anemia and gastrointestinal (GI) mucosal injury in the FES. Twenty-four male Kunming mice (20 ± 2 g) were randomly divided into control group and experimental group. The mice were placed in a footshock chamber that can generate 0.5 mA electrical impulse periodically for 0.5 h. After the process, red blood cell count, hemoglobin concentration and hematocrit, the levels of corticotropin releasing hormone (CRH) in serum and hypothalamus, and adrenocorticotropic hormone (ACTH) in serum and pituitary were detected separately. In addition, we investigated the expressions of inflammatory cytokines (IL-1, IL-6, TNF-α, iNOS, and IL-10) in the hypothalamus and duodenum by Polymerase Chain Reaction (PCR). Results showed that this FES model induced anemia, increased CRH and ACTH activity in the serum after the FES. Moreover, the expressions of IL-1β, IL-6, TNF-α, and iNOS were significantly increased following the process, while IL-10 was not activated. These findings suggest that anemia, the inflammatory cytokines in the hypothalamus and duodenum of the mice in the model induced by FES is closely related to GI mucosal injury/bleeding. Taken together, these results underscore the importance of anemia, GI mucosal injury/bleeding and stress, future studies would be needed to translate these findings into the benefit of affected patients.
format	article
author	Fangcheng Fan Yangwen Ai Ting Sun Shuran Li Hua Liu Xiaojie Shi Ziqian Zhang Qingshan Liu Yong Cheng
author_facet	Fangcheng Fan Yangwen Ai Ting Sun Shuran Li Hua Liu Xiaojie Shi Ziqian Zhang Qingshan Liu Yong Cheng
author_sort	Fangcheng Fan
title	The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
title_short	The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
title_full	The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
title_fullStr	The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
title_full_unstemmed	The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
title_sort	role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d64b4bf6350415888ff08e0a4af53e8
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The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation

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