Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo

Abstract Membrane-bound extracellular vesicles (EVs) mediate intercellular communication in all organisms, and those produced by placental mammals have become increasingly recognized as significant mediators of fetal-maternal communication. Here, we aimed to identify maternal cells targeted by place...

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Autores principales:	Sean L. Nguyen, Soo Hyun Ahn, Jacob W. Greenberg, Benjamin W. Collaer, Dalen W. Agnew, Ripla Arora, Margaret G. Petroff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d676c007dda4327bd0f8d592bb8d9ca
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spelling	oai:doaj.org-article:2d676c007dda4327bd0f8d592bb8d9ca2021-12-02T10:54:23ZIntegrins mediate placental extracellular vesicle trafficking to lung and liver in vivo10.1038/s41598-021-82752-w2045-2322https://doaj.org/article/2d676c007dda4327bd0f8d592bb8d9ca2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82752-whttps://doaj.org/toc/2045-2322Abstract Membrane-bound extracellular vesicles (EVs) mediate intercellular communication in all organisms, and those produced by placental mammals have become increasingly recognized as significant mediators of fetal-maternal communication. Here, we aimed to identify maternal cells targeted by placental EVs and elucidate the mechanisms by which they traffic to these cells. Exogenously administered pregnancy-associated EVs traffic specifically to the lung; further, placental EVs associate with lung interstitial macrophages and liver Kupffer cells in an integrin-dependent manner. Localization of EV to maternal lungs was confirmed in unmanipulated pregnancy using a transgenic reporter mouse model, which also provided in situ and in vitro evidence that fetally-derived EVs, rarely, may cause genetic alteration of maternal cells. These results provide for the first time direct in vivo evidence that placental EVs target maternal immune cells, and further, that EVs can alter cellular phenotype.Sean L. NguyenSoo Hyun AhnJacob W. GreenbergBenjamin W. CollaerDalen W. AgnewRipla AroraMargaret G. PetroffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sean L. Nguyen Soo Hyun Ahn Jacob W. Greenberg Benjamin W. Collaer Dalen W. Agnew Ripla Arora Margaret G. Petroff Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
description	Abstract Membrane-bound extracellular vesicles (EVs) mediate intercellular communication in all organisms, and those produced by placental mammals have become increasingly recognized as significant mediators of fetal-maternal communication. Here, we aimed to identify maternal cells targeted by placental EVs and elucidate the mechanisms by which they traffic to these cells. Exogenously administered pregnancy-associated EVs traffic specifically to the lung; further, placental EVs associate with lung interstitial macrophages and liver Kupffer cells in an integrin-dependent manner. Localization of EV to maternal lungs was confirmed in unmanipulated pregnancy using a transgenic reporter mouse model, which also provided in situ and in vitro evidence that fetally-derived EVs, rarely, may cause genetic alteration of maternal cells. These results provide for the first time direct in vivo evidence that placental EVs target maternal immune cells, and further, that EVs can alter cellular phenotype.
format	article
author	Sean L. Nguyen Soo Hyun Ahn Jacob W. Greenberg Benjamin W. Collaer Dalen W. Agnew Ripla Arora Margaret G. Petroff
author_facet	Sean L. Nguyen Soo Hyun Ahn Jacob W. Greenberg Benjamin W. Collaer Dalen W. Agnew Ripla Arora Margaret G. Petroff
author_sort	Sean L. Nguyen
title	Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
title_short	Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
title_full	Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
title_fullStr	Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
title_full_unstemmed	Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
title_sort	integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d676c007dda4327bd0f8d592bb8d9ca
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Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo

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