Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design

Abstract The overexpression of hoxd13a during zebrafish fin development causes distal endochondral expansion and simultaneous reduction of the finfold, mimicking the major events thought to have happened during the fin-to-limb transition in Vertebrates. We investigated the effect of hoxd13a overexpr...

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Autores principales:	João Castro, Vanessa Beviano, Ana Paço, Joana Leitão-Castro, Francisco Cadete, Miguel Francisco, Renata Freitas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d681f186e5c4e00baa83dc896ed4114
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spelling	oai:doaj.org-article:2d681f186e5c4e00baa83dc896ed41142021-12-02T14:25:21ZHoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design10.1038/s41598-021-86621-42045-2322https://doaj.org/article/2d681f186e5c4e00baa83dc896ed41142021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86621-4https://doaj.org/toc/2045-2322Abstract The overexpression of hoxd13a during zebrafish fin development causes distal endochondral expansion and simultaneous reduction of the finfold, mimicking the major events thought to have happened during the fin-to-limb transition in Vertebrates. We investigated the effect of hoxd13a overexpression on putative downstream targets and found it to cause downregulation of proximal fin identity markers (meis1 and emx2) and upregulation of genes involved in skeletogenesis/patterning (fbn1, dacha) and AER/Finfold maintenance (bmps). We then show that bmp2b overexpression leads to finfold reduction, recapitulating the phenotype observed in hoxd13a-overexpressing fins. In addition, we show that during the development of the long finfold in leot1/lofdt1 mutants, hoxd13a and bmp2b are downregulated. Our results suggest that modulation of the transcription factor Hoxd13 during evolution may have been involved in finfold reduction through regulation of the Bmp signalling that then activated apoptotic mechanisms impairing finfold elongation.João CastroVanessa BevianoAna PaçoJoana Leitão-CastroFrancisco CadeteMiguel FranciscoRenata FreitasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q João Castro Vanessa Beviano Ana Paço Joana Leitão-Castro Francisco Cadete Miguel Francisco Renata Freitas Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
description	Abstract The overexpression of hoxd13a during zebrafish fin development causes distal endochondral expansion and simultaneous reduction of the finfold, mimicking the major events thought to have happened during the fin-to-limb transition in Vertebrates. We investigated the effect of hoxd13a overexpression on putative downstream targets and found it to cause downregulation of proximal fin identity markers (meis1 and emx2) and upregulation of genes involved in skeletogenesis/patterning (fbn1, dacha) and AER/Finfold maintenance (bmps). We then show that bmp2b overexpression leads to finfold reduction, recapitulating the phenotype observed in hoxd13a-overexpressing fins. In addition, we show that during the development of the long finfold in leot1/lofdt1 mutants, hoxd13a and bmp2b are downregulated. Our results suggest that modulation of the transcription factor Hoxd13 during evolution may have been involved in finfold reduction through regulation of the Bmp signalling that then activated apoptotic mechanisms impairing finfold elongation.
format	article
author	João Castro Vanessa Beviano Ana Paço Joana Leitão-Castro Francisco Cadete Miguel Francisco Renata Freitas
author_facet	João Castro Vanessa Beviano Ana Paço Joana Leitão-Castro Francisco Cadete Miguel Francisco Renata Freitas
author_sort	João Castro
title	Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
title_short	Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
title_full	Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
title_fullStr	Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
title_full_unstemmed	Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
title_sort	hoxd13/bmp2-mediated mechanism involved in zebrafish finfold design
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d681f186e5c4e00baa83dc896ed4114
work_keys_str_mv	AT joaocastro hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign AT vanessabeviano hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign AT anapaco hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign AT joanaleitaocastro hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign AT franciscocadete hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign AT miguelfrancisco hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign AT renatafreitas hoxd13bmp2mediatedmechanisminvolvedinzebrafishfinfolddesign
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Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design

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