Impression cytology implicates cell autophagy in aqueous deficiency dry eye

Tony Lin,1,2,* Richard Filek,3,* Joy M Wang,4 Chong H Wu,4 Hong Liu,2 Cindy ML Hutnik1–3 1Department of Ophthalmology, Western University, 2Ivey Eye Institute, St Joseph’s Health Care, St Joseph’s Hospital, 3Department of Pathology and Laboratory Medicine, 4De...

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Autores principales:	Lin T, Filek R, Wang JM, Wu CH, Liu H, Hutnik CML
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	impression cytology cell autophagy dry eye technique schirmer test LC3 Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/2d71c3e6cb434933af282dbf797a5fb9
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spelling	oai:doaj.org-article:2d71c3e6cb434933af282dbf797a5fb92021-12-02T05:24:58ZImpression cytology implicates cell autophagy in aqueous deficiency dry eye1177-5483https://doaj.org/article/2d71c3e6cb434933af282dbf797a5fb92017-04-01T00:00:00Zhttps://www.dovepress.com/impression-cytology-implicates-cell-autophagy-in-aqueous-deficiency-dr-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Tony Lin,1,2,* Richard Filek,3,* Joy M Wang,4 Chong H Wu,4 Hong Liu,2 Cindy ML Hutnik1–3 1Department of Ophthalmology, Western University, 2Ivey Eye Institute, St Joseph’s Health Care, St Joseph’s Hospital, 3Department of Pathology and Laboratory Medicine, 4Department of Biology, Western University, London, ON, Canada *These authors contributed equally to this work Purpose: Impression cytology in combination with a cell autophagy marker was used to demonstrate the utility of a novel frozen section technique, to assess the health of the ocular surface, as well as to correlate autophagic cell death with the commonly used clinical tests for dry eye. Methods: Female patients over the age of 18 years who attended an outpatient ophthalmology clinic were invited to participate. Schirmer’s test, tear film break-up time and the ocular surface disease index questionnaire were used as objective and subjective clinical tests for dry eye. The cellular biomarker microtubule-associated protein light chain 3 (LC3) was used as a marker of autophagic cell death. Results: Low LC3 nuclear staining was positively correlated with Schirmer’s test <10 mm. No correlation was found between other clinical tests for dry eye. Conclusion: This study demonstrates a positive linear relationship between Schirmer’s testing and LC3. There is a lack of correlation between the commonly used diagnostics tests for dry eye, highlighting our incomplete understanding and assessment of dry eye. Keywords: impression cytology, cell autophagy, dry eye, technique, Schirmer’s test, LC3Lin TFilek RWang JMWu CHLiu HHutnik CMLDove Medical Pressarticleimpression cytologycell autophagydry eyetechniqueschirmer testLC3OphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 11, Pp 773-779 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	impression cytology cell autophagy dry eye technique schirmer test LC3 Ophthalmology RE1-994
spellingShingle	impression cytology cell autophagy dry eye technique schirmer test LC3 Ophthalmology RE1-994 Lin T Filek R Wang JM Wu CH Liu H Hutnik CML Impression cytology implicates cell autophagy in aqueous deficiency dry eye
description	Tony Lin,1,2,* Richard Filek,3,* Joy M Wang,4 Chong H Wu,4 Hong Liu,2 Cindy ML Hutnik1–3 1Department of Ophthalmology, Western University, 2Ivey Eye Institute, St Joseph’s Health Care, St Joseph’s Hospital, 3Department of Pathology and Laboratory Medicine, 4Department of Biology, Western University, London, ON, Canada *These authors contributed equally to this work Purpose: Impression cytology in combination with a cell autophagy marker was used to demonstrate the utility of a novel frozen section technique, to assess the health of the ocular surface, as well as to correlate autophagic cell death with the commonly used clinical tests for dry eye. Methods: Female patients over the age of 18 years who attended an outpatient ophthalmology clinic were invited to participate. Schirmer’s test, tear film break-up time and the ocular surface disease index questionnaire were used as objective and subjective clinical tests for dry eye. The cellular biomarker microtubule-associated protein light chain 3 (LC3) was used as a marker of autophagic cell death. Results: Low LC3 nuclear staining was positively correlated with Schirmer’s test <10 mm. No correlation was found between other clinical tests for dry eye. Conclusion: This study demonstrates a positive linear relationship between Schirmer’s testing and LC3. There is a lack of correlation between the commonly used diagnostics tests for dry eye, highlighting our incomplete understanding and assessment of dry eye. Keywords: impression cytology, cell autophagy, dry eye, technique, Schirmer’s test, LC3
format	article
author	Lin T Filek R Wang JM Wu CH Liu H Hutnik CML
author_facet	Lin T Filek R Wang JM Wu CH Liu H Hutnik CML
author_sort	Lin T
title	Impression cytology implicates cell autophagy in aqueous deficiency dry eye
title_short	Impression cytology implicates cell autophagy in aqueous deficiency dry eye
title_full	Impression cytology implicates cell autophagy in aqueous deficiency dry eye
title_fullStr	Impression cytology implicates cell autophagy in aqueous deficiency dry eye
title_full_unstemmed	Impression cytology implicates cell autophagy in aqueous deficiency dry eye
title_sort	impression cytology implicates cell autophagy in aqueous deficiency dry eye
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/2d71c3e6cb434933af282dbf797a5fb9
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_version_	1718400400406609920

Impression cytology implicates cell autophagy in aqueous deficiency dry eye

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