Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes

Abstract Honey bee queens undergo dramatic behavioral (e.g., reduced sexual receptivity), physiological (e.g., ovary activation, ovulation, and modulation of pheromone production) and transcriptional changes after they complete mating. To elucidate how queen post-mating changes are influenced by sem...

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Autores principales:	W. Cameron Jasper, Laura M. Brutscher, Christina M. Grozinger, Elina L. Niño
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d79997216f94594be08406ec803ee47
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spelling	oai:doaj.org-article:2d79997216f94594be08406ec803ee472021-12-02T17:55:13ZInjection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes10.1038/s41598-020-68437-w2045-2322https://doaj.org/article/2d79997216f94594be08406ec803ee472020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68437-whttps://doaj.org/toc/2045-2322Abstract Honey bee queens undergo dramatic behavioral (e.g., reduced sexual receptivity), physiological (e.g., ovary activation, ovulation, and modulation of pheromone production) and transcriptional changes after they complete mating. To elucidate how queen post-mating changes are influenced by seminal fluid, the non-spermatozoa-containing component of semen, we injected queens with semen or seminal fluid alone. We assessed queen sexual receptivity (as measured by likelihood to take mating flights), ovary activation, worker retinue response (which is influenced by queen pheromone production), and transcriptional changes in queen abdominal fat body and brain tissues. Injection with either seminal fluid or semen resulted in decreased sexual receptivity, increased attractiveness of queens to workers, and altered expression of several genes that are also regulated by natural mating in queens. The post-mating and transcriptional changes of queens receiving seminal fluid were not significantly different from queens injected with semen, suggesting that components in seminal fluid, such as seminal fluid proteins, are largely responsible for stimulating post-mating changes in queens.W. Cameron JasperLaura M. BrutscherChristina M. GrozingerElina L. NiñoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-18 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q W. Cameron Jasper Laura M. Brutscher Christina M. Grozinger Elina L. Niño Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
description	Abstract Honey bee queens undergo dramatic behavioral (e.g., reduced sexual receptivity), physiological (e.g., ovary activation, ovulation, and modulation of pheromone production) and transcriptional changes after they complete mating. To elucidate how queen post-mating changes are influenced by seminal fluid, the non-spermatozoa-containing component of semen, we injected queens with semen or seminal fluid alone. We assessed queen sexual receptivity (as measured by likelihood to take mating flights), ovary activation, worker retinue response (which is influenced by queen pheromone production), and transcriptional changes in queen abdominal fat body and brain tissues. Injection with either seminal fluid or semen resulted in decreased sexual receptivity, increased attractiveness of queens to workers, and altered expression of several genes that are also regulated by natural mating in queens. The post-mating and transcriptional changes of queens receiving seminal fluid were not significantly different from queens injected with semen, suggesting that components in seminal fluid, such as seminal fluid proteins, are largely responsible for stimulating post-mating changes in queens.
format	article
author	W. Cameron Jasper Laura M. Brutscher Christina M. Grozinger Elina L. Niño
author_facet	W. Cameron Jasper Laura M. Brutscher Christina M. Grozinger Elina L. Niño
author_sort	W. Cameron Jasper
title	Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
title_short	Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
title_full	Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
title_fullStr	Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
title_full_unstemmed	Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
title_sort	injection of seminal fluid into the hemocoel of honey bee queens (apis mellifera) can stimulate post-mating changes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2d79997216f94594be08406ec803ee47
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Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes

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