Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children

Vaithinathan Selvaraju,1 Chandra MK Venkatapoorna,1 Jeganathan R Babu,1,2 Thangiah Geetha1,2 1Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USA; 2Boshell Metabolic Diseases and Diabetes Program, Auburn University, Auburn, AL, USACorrespondence: Thangi...

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Autores principales:	Selvaraju V, Venkatapoorna CMK, Babu JR, Geetha T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	amy1 copy numbers obesity-related markers inflammatory markers childhood obesity body mass index Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/2d7c5f44b48245ab802853ddb452a6b8
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spelling	oai:doaj.org-article:2d7c5f44b48245ab802853ddb452a6b82021-12-02T09:03:53ZSalivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children1178-7007https://doaj.org/article/2d7c5f44b48245ab802853ddb452a6b82020-05-01T00:00:00Zhttps://www.dovepress.com/salivary-amylase-gene-copy-number-is-associated-with-the-obesity-and-i-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Vaithinathan Selvaraju,1 Chandra MK Venkatapoorna,1 Jeganathan R Babu,1,2 Thangiah Geetha1,2 1Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USA; 2Boshell Metabolic Diseases and Diabetes Program, Auburn University, Auburn, AL, USACorrespondence: Thangiah GeethaDepartment of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USATel +1 334 844 7418Fax +1 334 844 3268Email thangge@auburn.eduBackground: We have recently shown that the copy number of salivary amylase (AMY1) gene was significantly decreased, and the obesity-related salivary biomarkers resistin, MCP-1, TNF-α, IL-6, and CRP were signiﬁcantly increased in overweight/obese children compared to normal weight. This study aimed to evaluate the association of AMY1 copy number variant (CNV) with obesity and inflammatory markers. Seventy-six participants aged between 6 and 10 years have participated, and the saliva samples were collected along with the anthropometric measurements.Methods: AMY1 copy number was analyzed by 3D digital PCR, and obesity-related biomarkers were performed with a Bioplex multiplex analyzer.Results: The mean AMY1 copy number was higher in normal weight (7.90 ± 0.38) compared to the overweight/obese group (6.20 ± 0.29). The association of AMY1 CNV with obesity and inflammatory markers showed significant negative correlation [CRP, β = − 0.238 (p < 0.05); resistin, β = − 0.25 (p < 0.05); MCP-1, β = − 0.304 (p < 0.01)] except for complement factor D, TNF α and IL-6. The anti-inflammatory cytokine, IL-10 reported a positive correlation with AMY1 copy number with a β = 0.268 (p < 0.05). The multivariable model adjusted with age and gender depicted a similar correlation with obesity markers.Conclusion: Our results report that AMY1 CNV is associated with obesity and inflammatory biomarkers in children’s saliva sample.Keywords: AMY1 copy numbers, obesity-related markers, inflammatory markers, childhood obesity, body mass indexSelvaraju VVenkatapoorna CMKBabu JRGeetha TDove Medical Pressarticleamy1 copy numbersobesity-related markersinflammatory markerschildhood obesitybody mass indexSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 1695-1701 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	amy1 copy numbers obesity-related markers inflammatory markers childhood obesity body mass index Specialties of internal medicine RC581-951
spellingShingle	amy1 copy numbers obesity-related markers inflammatory markers childhood obesity body mass index Specialties of internal medicine RC581-951 Selvaraju V Venkatapoorna CMK Babu JR Geetha T Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children
description	Vaithinathan Selvaraju,1 Chandra MK Venkatapoorna,1 Jeganathan R Babu,1,2 Thangiah Geetha1,2 1Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USA; 2Boshell Metabolic Diseases and Diabetes Program, Auburn University, Auburn, AL, USACorrespondence: Thangiah GeethaDepartment of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USATel +1 334 844 7418Fax +1 334 844 3268Email thangge@auburn.eduBackground: We have recently shown that the copy number of salivary amylase (AMY1) gene was significantly decreased, and the obesity-related salivary biomarkers resistin, MCP-1, TNF-α, IL-6, and CRP were signiﬁcantly increased in overweight/obese children compared to normal weight. This study aimed to evaluate the association of AMY1 copy number variant (CNV) with obesity and inflammatory markers. Seventy-six participants aged between 6 and 10 years have participated, and the saliva samples were collected along with the anthropometric measurements.Methods: AMY1 copy number was analyzed by 3D digital PCR, and obesity-related biomarkers were performed with a Bioplex multiplex analyzer.Results: The mean AMY1 copy number was higher in normal weight (7.90 ± 0.38) compared to the overweight/obese group (6.20 ± 0.29). The association of AMY1 CNV with obesity and inflammatory markers showed significant negative correlation [CRP, β = − 0.238 (p < 0.05); resistin, β = − 0.25 (p < 0.05); MCP-1, β = − 0.304 (p < 0.01)] except for complement factor D, TNF α and IL-6. The anti-inflammatory cytokine, IL-10 reported a positive correlation with AMY1 copy number with a β = 0.268 (p < 0.05). The multivariable model adjusted with age and gender depicted a similar correlation with obesity markers.Conclusion: Our results report that AMY1 CNV is associated with obesity and inflammatory biomarkers in children’s saliva sample.Keywords: AMY1 copy numbers, obesity-related markers, inflammatory markers, childhood obesity, body mass index
format	article
author	Selvaraju V Venkatapoorna CMK Babu JR Geetha T
author_facet	Selvaraju V Venkatapoorna CMK Babu JR Geetha T
author_sort	Selvaraju V
title	Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children
title_short	Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children
title_full	Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children
title_fullStr	Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children
title_full_unstemmed	Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children
title_sort	salivary amylase gene copy number is associated with the obesity and inflammatory markers in children
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/2d7c5f44b48245ab802853ddb452a6b8
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Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children

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