Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications

Samer Hasan Hussein-Al-Ali,1,2 Mohamed Ezzat El Zowalaty,3,4 Mohd Zobir Hussein,5 Benjamin M Geilich,6 Thomas J Webster6,7 1Laboratory of Molecular Biomedicine, 2Faculty of Pharmacy, Isra University, Amman, Kingdom of Jordan; 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience,...

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Autores principales:	Hussein-Al-Ali SH, El Zowalaty ME, Hussein MZ, Geilich BM, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2d7f1fae21644f3db6f78d1dc03f61f8
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spelling	oai:doaj.org-article:2d7f1fae21644f3db6f78d1dc03f61f82021-12-02T07:44:03ZSynthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications1178-2013https://doaj.org/article/2d7f1fae21644f3db6f78d1dc03f61f82014-08-01T00:00:00Zhttp://www.dovepress.com/synthesis-characterization-and-antimicrobial-activity-of-an-ampicillin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Samer Hasan Hussein-Al-Ali,1,2 Mohamed Ezzat El Zowalaty,3,4 Mohd Zobir Hussein,5 Benjamin M Geilich,6 Thomas J Webster6,7 1Laboratory of Molecular Biomedicine, 2Faculty of Pharmacy, Isra University, Amman, Kingdom of Jordan; 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 4Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia; 5Materials Synthesis and Characterization Laboratory Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 6Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA; 7Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia Abstract: Because of their magnetic properties, magnetic nanoparticles (MNPs) have numerous diverse biomedical applications. In addition, because of their ability to penetrate bacteria and biofilms, nanoantimicrobial agents have become increasingly popular for the control of infectious diseases. Here, MNPs were prepared through an iron salt coprecipitation method in an alkaline medium, followed by a chitosan coating step (CS-coated MNPs); finally, the MNPs were loaded with ampicillin (amp) to form an amp-CS-MNP nanocomposite. Both the MNPs and amp-CS-MNPs were subsequently characterized and evaluated for their antibacterial activity. X-ray diffraction results showed that the MNPs and nanocomposites were composed of pure magnetite. Fourier transform infrared spectra and thermogravimetric data for the MNPs, CS-coated MNPs, and amp-CS-MNP nanocomposite were compared, which confirmed the CS coating on the MNPs and the amp-loaded nanocomposite. Magnetization curves showed that both the MNPs and the amp-CS-MNP nanocomposites were superparamagnetic, with saturation magnetizations at 80.1 and 26.6 emu g-1, respectively. Amp was loaded at 8.3%. Drug release was also studied, and the total release equilibrium for amp from the amp-CS-MNPs was 100% over 400 minutes. In addition, the antimicrobial activity of the amp-CS-MNP nanocomposite was determined using agar diffusion and growth inhibition assays against Gram-positive bacteria and Gram-negative bacteria, as well as Candida albicans. The minimum inhibitory concentration of the amp-CS-MNP nanocomposite was determined against bacteria including Mycobacterium tuberculosis. The synthesized nanocomposites exhibited antibacterial and antifungal properties, as well as antimycobacterial effects. Thus, this study introduces a novel ß-lactam antibacterial-based nanocomposite that can decrease fungus activity on demand for numerous medical applications. Keywords: iron oxide nanoparticles, chitosan, coating material, antibacterial activity, ß-lactam, and nanoantibioticsHussein-Al-Ali SHEl Zowalaty MEHussein MZGeilich BMWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3801-3814 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hussein-Al-Ali SH El Zowalaty ME Hussein MZ Geilich BM Webster TJ Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
description	Samer Hasan Hussein-Al-Ali,1,2 Mohamed Ezzat El Zowalaty,3,4 Mohd Zobir Hussein,5 Benjamin M Geilich,6 Thomas J Webster6,7 1Laboratory of Molecular Biomedicine, 2Faculty of Pharmacy, Isra University, Amman, Kingdom of Jordan; 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 4Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia; 5Materials Synthesis and Characterization Laboratory Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 6Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA; 7Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia Abstract: Because of their magnetic properties, magnetic nanoparticles (MNPs) have numerous diverse biomedical applications. In addition, because of their ability to penetrate bacteria and biofilms, nanoantimicrobial agents have become increasingly popular for the control of infectious diseases. Here, MNPs were prepared through an iron salt coprecipitation method in an alkaline medium, followed by a chitosan coating step (CS-coated MNPs); finally, the MNPs were loaded with ampicillin (amp) to form an amp-CS-MNP nanocomposite. Both the MNPs and amp-CS-MNPs were subsequently characterized and evaluated for their antibacterial activity. X-ray diffraction results showed that the MNPs and nanocomposites were composed of pure magnetite. Fourier transform infrared spectra and thermogravimetric data for the MNPs, CS-coated MNPs, and amp-CS-MNP nanocomposite were compared, which confirmed the CS coating on the MNPs and the amp-loaded nanocomposite. Magnetization curves showed that both the MNPs and the amp-CS-MNP nanocomposites were superparamagnetic, with saturation magnetizations at 80.1 and 26.6 emu g-1, respectively. Amp was loaded at 8.3%. Drug release was also studied, and the total release equilibrium for amp from the amp-CS-MNPs was 100% over 400 minutes. In addition, the antimicrobial activity of the amp-CS-MNP nanocomposite was determined using agar diffusion and growth inhibition assays against Gram-positive bacteria and Gram-negative bacteria, as well as Candida albicans. The minimum inhibitory concentration of the amp-CS-MNP nanocomposite was determined against bacteria including Mycobacterium tuberculosis. The synthesized nanocomposites exhibited antibacterial and antifungal properties, as well as antimycobacterial effects. Thus, this study introduces a novel ß-lactam antibacterial-based nanocomposite that can decrease fungus activity on demand for numerous medical applications. Keywords: iron oxide nanoparticles, chitosan, coating material, antibacterial activity, ß-lactam, and nanoantibiotics
format	article
author	Hussein-Al-Ali SH El Zowalaty ME Hussein MZ Geilich BM Webster TJ
author_facet	Hussein-Al-Ali SH El Zowalaty ME Hussein MZ Geilich BM Webster TJ
author_sort	Hussein-Al-Ali SH
title	Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
title_short	Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
title_full	Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
title_fullStr	Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
title_full_unstemmed	Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
title_sort	synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/2d7f1fae21644f3db6f78d1dc03f61f8
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Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications

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