Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells

Si-Feng Shi,1 Jing-Fu Jia,2 Xiao-Kui Guo,3 Ya-Ping Zhao,2 De-Sheng Chen,1 Yong-Yuan Guo,1 Tao Cheng,1 Xian-Long Zhang11Department of Orthopedic Surgery, Shanghai Sixth People&rsquo;s Hospital, School of Medicine, 2School of Chemistry and Chemical Technology, 3Department of Medical Microbiolo...

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Autores principales:	Shi S, Jia J, Guo X, Zhao Y, Chen D, Guo Y, Cheng T, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2d85dcd0e57b493da7cc52f31b50242e
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spelling	oai:doaj.org-article:2d85dcd0e57b493da7cc52f31b50242e2021-12-02T05:14:28ZBiocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells1176-91141178-2013https://doaj.org/article/2d85dcd0e57b493da7cc52f31b50242e2012-10-01T00:00:00Zhttp://www.dovepress.com/biocompatibility-of-chitosan-coated-iron-oxide-nanoparticles-with-oste-a11355https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Si-Feng Shi,1 Jing-Fu Jia,2 Xiao-Kui Guo,3 Ya-Ping Zhao,2 De-Sheng Chen,1 Yong-Yuan Guo,1 Tao Cheng,1 Xian-Long Zhang11Department of Orthopedic Surgery, Shanghai Sixth People&rsquo;s Hospital, School of Medicine, 2School of Chemistry and Chemical Technology, 3Department of Medical Microbiology and Parasitology, School of Medicine, Shanghai Jiao Tong University Shanghai, ChinaBackground: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease. Chitosan is widely used in the biomedical community, including for orthopedic applications. The aim of the present study was to coat chitosan onto iron oxide nanoparticles and to determine its effect on the proliferation and differentiation of osteoblasts.Methods: Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, and vibrating sample magnetometry. Uptake of nanoparticles by osteoblasts was studied by transmission electron microscopy and Prussian blue staining. Viability and proliferation of osteoblasts were measured in the presence of uncoated iron oxide magnetic nanoparticles or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, and extracellular calcium deposition was studied in the presence of the nanoparticles.Results: Chitosan-coated iron oxide nanoparticles enhanced osteoblast proliferation, decreased cell membrane damage, and promoted cell differentiation, as indicated by an increase in alkaline phosphatase and extracellular calcium deposition. Chitosan-coated iron oxide nanoparticles showed good compatibility with osteoblasts.Conclusion: Further research is necessary to optimize magnetic nanoparticles for the treatment of bone disease.Keywords: chitosan-coated iron oxide, magnetic nanoparticles, osteoblastsShi SJia JGuo XZhao YChen DGuo YCheng TZhang XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5593-5602 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shi S Jia J Guo X Zhao Y Chen D Guo Y Cheng T Zhang X Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
description	Si-Feng Shi,1 Jing-Fu Jia,2 Xiao-Kui Guo,3 Ya-Ping Zhao,2 De-Sheng Chen,1 Yong-Yuan Guo,1 Tao Cheng,1 Xian-Long Zhang11Department of Orthopedic Surgery, Shanghai Sixth People&rsquo;s Hospital, School of Medicine, 2School of Chemistry and Chemical Technology, 3Department of Medical Microbiology and Parasitology, School of Medicine, Shanghai Jiao Tong University Shanghai, ChinaBackground: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease. Chitosan is widely used in the biomedical community, including for orthopedic applications. The aim of the present study was to coat chitosan onto iron oxide nanoparticles and to determine its effect on the proliferation and differentiation of osteoblasts.Methods: Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, and vibrating sample magnetometry. Uptake of nanoparticles by osteoblasts was studied by transmission electron microscopy and Prussian blue staining. Viability and proliferation of osteoblasts were measured in the presence of uncoated iron oxide magnetic nanoparticles or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, and extracellular calcium deposition was studied in the presence of the nanoparticles.Results: Chitosan-coated iron oxide nanoparticles enhanced osteoblast proliferation, decreased cell membrane damage, and promoted cell differentiation, as indicated by an increase in alkaline phosphatase and extracellular calcium deposition. Chitosan-coated iron oxide nanoparticles showed good compatibility with osteoblasts.Conclusion: Further research is necessary to optimize magnetic nanoparticles for the treatment of bone disease.Keywords: chitosan-coated iron oxide, magnetic nanoparticles, osteoblasts
format	article
author	Shi S Jia J Guo X Zhao Y Chen D Guo Y Cheng T Zhang X
author_facet	Shi S Jia J Guo X Zhao Y Chen D Guo Y Cheng T Zhang X
author_sort	Shi S
title	Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_short	Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_full	Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_fullStr	Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_full_unstemmed	Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_sort	biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/2d85dcd0e57b493da7cc52f31b50242e
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Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells

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