Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)

Abstract. Darmawan C, Wiendi NMA, Utomo C, Liwang T. 2020. Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis). Biodiversitas 21: 3720-3726. Novel traits introduction to existing varieties may shorten the duration of oil palm genetic improvement. This approach heavily rel...

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Autores principales:	Chris Darmawan, Ni Made Armini Wiendi, Condro Utomo, Tony Liwang
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	electroporation, genetic engineering, oil palm, transformation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2d90e61c9f5c43ccbb5bb0a7d73bddf5
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spelling	oai:doaj.org-article:2d90e61c9f5c43ccbb5bb0a7d73bddf52021-11-22T12:17:57ZElectroporation-mediated genetic transformation of oil palm (Elaeis guineensis)1412-033X2085-472210.13057/biodiv/d210839https://doaj.org/article/2d90e61c9f5c43ccbb5bb0a7d73bddf52020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6187https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Darmawan C, Wiendi NMA, Utomo C, Liwang T. 2020. Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis). Biodiversitas 21: 3720-3726. Novel traits introduction to existing varieties may shorten the duration of oil palm genetic improvement. This approach heavily relies on efficient plant transformation and regeneration methods. Commonly used methods such as biolistic and Agrobacterium-mediated transformation still generated low efficiency for oil palm. Therefore, it is important to find alternative transformation methods that have the potency to give better results. Electroporation is usually used for delivering genes into bacteria or plant protoplast where cell wall does not exist, however, in this research, it was used to deliver plasmid into plant tissues. The aim of this research is to establish an optimum electroporation protocol for delivering genes into oil palm calli. Electric field strength of 250, 500, 750, 1,000, and 1,250 V/cm were applied for oil palm calli electroporation. Transient GUS assay was not applicable for initial detection presumably due to oil palm endogenous GUS-like protein activity after electroporation. Delivered gene could be detected in survived calli from all tested electric field strength treatments and the highest calli growth rates were observed from 250 V/cm electroporation treatment. Overall results showed that electroporation could be used to deliver specific genes into oil palm calli and might be developed further to increase its efficiency.Chris DarmawanNi Made Armini WiendiCondro UtomoTony LiwangMBI & UNS Soloarticleelectroporation, genetic engineering, oil palm, transformationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	electroporation, genetic engineering, oil palm, transformation Biology (General) QH301-705.5
spellingShingle	electroporation, genetic engineering, oil palm, transformation Biology (General) QH301-705.5 Chris Darmawan Ni Made Armini Wiendi Condro Utomo Tony Liwang Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)
description	Abstract. Darmawan C, Wiendi NMA, Utomo C, Liwang T. 2020. Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis). Biodiversitas 21: 3720-3726. Novel traits introduction to existing varieties may shorten the duration of oil palm genetic improvement. This approach heavily relies on efficient plant transformation and regeneration methods. Commonly used methods such as biolistic and Agrobacterium-mediated transformation still generated low efficiency for oil palm. Therefore, it is important to find alternative transformation methods that have the potency to give better results. Electroporation is usually used for delivering genes into bacteria or plant protoplast where cell wall does not exist, however, in this research, it was used to deliver plasmid into plant tissues. The aim of this research is to establish an optimum electroporation protocol for delivering genes into oil palm calli. Electric field strength of 250, 500, 750, 1,000, and 1,250 V/cm were applied for oil palm calli electroporation. Transient GUS assay was not applicable for initial detection presumably due to oil palm endogenous GUS-like protein activity after electroporation. Delivered gene could be detected in survived calli from all tested electric field strength treatments and the highest calli growth rates were observed from 250 V/cm electroporation treatment. Overall results showed that electroporation could be used to deliver specific genes into oil palm calli and might be developed further to increase its efficiency.
format	article
author	Chris Darmawan Ni Made Armini Wiendi Condro Utomo Tony Liwang
author_facet	Chris Darmawan Ni Made Armini Wiendi Condro Utomo Tony Liwang
author_sort	Chris Darmawan
title	Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)
title_short	Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)
title_full	Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)
title_fullStr	Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)
title_full_unstemmed	Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)
title_sort	electroporation-mediated genetic transformation of oil palm (elaeis guineensis)
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/2d90e61c9f5c43ccbb5bb0a7d73bddf5
work_keys_str_mv	AT chrisdarmawan electroporationmediatedgenetictransformationofoilpalmelaeisguineensis AT nimadearminiwiendi electroporationmediatedgenetictransformationofoilpalmelaeisguineensis AT condroutomo electroporationmediatedgenetictransformationofoilpalmelaeisguineensis AT tonyliwang electroporationmediatedgenetictransformationofoilpalmelaeisguineensis
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Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)

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