Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand

Abstract Leucocytozoon sabrazesi is the intracellular protozoa of leucocytozoonosis, which is transmitted by the insect vectors and affects chickens in most subtropical and tropical regions of the globe, except South America, and causing enormous economic losses due to decreasing meat yield and egg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Runglawan Chawengkirttikul, Witchuta Junsiri, Amaya Watthanadirek, Napassorn Poolsawat, Sutthida Minsakorn, Nitipon Srionrod, Panat Anuracpreeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d96b2d9ce304d87a919fdea03267193
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2d96b2d9ce304d87a919fdea03267193
record_format	dspace
spelling	oai:doaj.org-article:2d96b2d9ce304d87a919fdea032671932021-12-02T16:45:53ZMolecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand10.1038/s41598-021-96241-72045-2322https://doaj.org/article/2d96b2d9ce304d87a919fdea032671932021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96241-7https://doaj.org/toc/2045-2322Abstract Leucocytozoon sabrazesi is the intracellular protozoa of leucocytozoonosis, which is transmitted by the insect vectors and affects chickens in most subtropical and tropical regions of the globe, except South America, and causing enormous economic losses due to decreasing meat yield and egg production. In this study, L. sabrazesi gametocytes have been observed in the blood smears, and molecular methods have been used to analyse the occurrence and genetic diversity of L. sabrazesi in blood samples from 313 chickens raised in northern, western and southern parts of Thailand. The nested polymerase chain reaction (nested PCR) assay based on the cytb gene revealed that 80.51% (252/313) chickens were positive of L. sabrazesi. The phylogenetic analysis indicated that L. sabrazesi cytb gene is conserved in Thailand, showed 2 clades and 2 subclades with similarity ranged from 89.5 to 100%. The diversity analysis showed 13 and 18 haplotypes of the sequences from Thailand and from other countries, respectively. The entropy analyses of nucleic acid sequences showed 26 high entropy peaks with values ranging from 0.24493 to 1.21056, while those of amino acid sequences exhibited 5 high entropy peaks with values ranging from 0.39267 to 0.97012. The results; therefore, indicate a high molecular occurrence of L. sabrazesi in chicken blood samples with the associated factors that is statistically significant (p < 0.05). Hence, our results could be used to improve the immunodiagnostic methods and to find appropriate preventive control strategies or vaccination programs against leucocytozoonosis in order to mitigate or eliminate the harmful impact of this infection on chicken industry.Runglawan ChawengkirttikulWitchuta JunsiriAmaya WatthanadirekNapassorn PoolsawatSutthida MinsakornNitipon SrionrodPanat AnuracpreedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Runglawan Chawengkirttikul Witchuta Junsiri Amaya Watthanadirek Napassorn Poolsawat Sutthida Minsakorn Nitipon Srionrod Panat Anuracpreeda Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
description	Abstract Leucocytozoon sabrazesi is the intracellular protozoa of leucocytozoonosis, which is transmitted by the insect vectors and affects chickens in most subtropical and tropical regions of the globe, except South America, and causing enormous economic losses due to decreasing meat yield and egg production. In this study, L. sabrazesi gametocytes have been observed in the blood smears, and molecular methods have been used to analyse the occurrence and genetic diversity of L. sabrazesi in blood samples from 313 chickens raised in northern, western and southern parts of Thailand. The nested polymerase chain reaction (nested PCR) assay based on the cytb gene revealed that 80.51% (252/313) chickens were positive of L. sabrazesi. The phylogenetic analysis indicated that L. sabrazesi cytb gene is conserved in Thailand, showed 2 clades and 2 subclades with similarity ranged from 89.5 to 100%. The diversity analysis showed 13 and 18 haplotypes of the sequences from Thailand and from other countries, respectively. The entropy analyses of nucleic acid sequences showed 26 high entropy peaks with values ranging from 0.24493 to 1.21056, while those of amino acid sequences exhibited 5 high entropy peaks with values ranging from 0.39267 to 0.97012. The results; therefore, indicate a high molecular occurrence of L. sabrazesi in chicken blood samples with the associated factors that is statistically significant (p < 0.05). Hence, our results could be used to improve the immunodiagnostic methods and to find appropriate preventive control strategies or vaccination programs against leucocytozoonosis in order to mitigate or eliminate the harmful impact of this infection on chicken industry.
format	article
author	Runglawan Chawengkirttikul Witchuta Junsiri Amaya Watthanadirek Napassorn Poolsawat Sutthida Minsakorn Nitipon Srionrod Panat Anuracpreeda
author_facet	Runglawan Chawengkirttikul Witchuta Junsiri Amaya Watthanadirek Napassorn Poolsawat Sutthida Minsakorn Nitipon Srionrod Panat Anuracpreeda
author_sort	Runglawan Chawengkirttikul
title	Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
title_short	Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
title_full	Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
title_fullStr	Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
title_full_unstemmed	Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
title_sort	molecular detection and genetic diversity of leucocytozoon sabrazesi in chickens in thailand
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d96b2d9ce304d87a919fdea03267193
work_keys_str_mv	AT runglawanchawengkirttikul moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand AT witchutajunsiri moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand AT amayawatthanadirek moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand AT napassornpoolsawat moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand AT sutthidaminsakorn moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand AT nitiponsrionrod moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand AT panatanuracpreeda moleculardetectionandgeneticdiversityofleucocytozoonsabrazesiinchickensinthailand
_version_	1718383445236776960

Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand

Ejemplares similares