Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro

Abstract Kava is a beverage made from the ground roots of the plant Piper Methysticum. Active compounds of Kava have previously been demonstrated to exert an antiproliferative effect through cell cycle arrest and promotion of apoptosis. Our aim was to investigate the in vitro effects of the main con...

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Autores principales:	Antonio Celentano, Callisthenis Yiannis, Rita Paolini, Pangzhen Zhang, Camile S. Farah, Nicola Cirillo, Tami Yap, Michael McCullough
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d9f9316fc9c4a0e93d2b5b6c43e8848
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spelling	oai:doaj.org-article:2d9f9316fc9c4a0e93d2b5b6c43e88482021-12-02T18:51:27ZKava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro10.1038/s41598-020-73058-42045-2322https://doaj.org/article/2d9f9316fc9c4a0e93d2b5b6c43e88482020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73058-4https://doaj.org/toc/2045-2322Abstract Kava is a beverage made from the ground roots of the plant Piper Methysticum. Active compounds of Kava have previously been demonstrated to exert an antiproliferative effect through cell cycle arrest and promotion of apoptosis. Our aim was to investigate the in vitro effects of the main constituents derived from Kava on oral squamous cell carcinoma (OSCC) activity. Gas chromatography mass spectrometry (GCMS) was used to characterise the main constituents of two Kava preparations. Cell proliferation was assessed in two human OSCC cell lines (H400 and BICR56) and in normal oral keratinocytes (OKF6) treated with the identified Kava constituents, namely Flavokawain A (FKA), Flavokawain B (FKB), yangonin, kavain and methysticin using an MTS in vitro assay. Cell migration at 16 h was assessed using a Transwell migration assay. Cell invasion was measured at 22 h using a Matrigel assay. Cell adhesion was assessed at 90 min with a Cytoselect Adhesion assay. The two Kava preparations contained substantially different concentrations of the main chemical constituents. Treatment of malignant and normal oral keratinocyte cell lines with three of the identified constituents, 10 μg/ml FKA, 2.5 μg/ml FKB and 10 μg/ml yangonin, showed a significant reduction in cell proliferation in both H400 and BICR56 cancer cell lines but not in normal OKF6 cells. Remarkably, the same Kava constituents induced a significant reduction of OSCC cell migration and invasion. We have demonstrated, for the first time, that Kava constituents, FKA, FKB and yangonin have potential anticancer effects on OSCC. This highlights an avenue for further research of Kava constituents in the development of future cancer therapies to prevent and treat OSCC.Antonio CelentanoCallisthenis YiannisRita PaoliniPangzhen ZhangCamile S. FarahNicola CirilloTami YapMichael McCulloughNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonio Celentano Callisthenis Yiannis Rita Paolini Pangzhen Zhang Camile S. Farah Nicola Cirillo Tami Yap Michael McCullough Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
description	Abstract Kava is a beverage made from the ground roots of the plant Piper Methysticum. Active compounds of Kava have previously been demonstrated to exert an antiproliferative effect through cell cycle arrest and promotion of apoptosis. Our aim was to investigate the in vitro effects of the main constituents derived from Kava on oral squamous cell carcinoma (OSCC) activity. Gas chromatography mass spectrometry (GCMS) was used to characterise the main constituents of two Kava preparations. Cell proliferation was assessed in two human OSCC cell lines (H400 and BICR56) and in normal oral keratinocytes (OKF6) treated with the identified Kava constituents, namely Flavokawain A (FKA), Flavokawain B (FKB), yangonin, kavain and methysticin using an MTS in vitro assay. Cell migration at 16 h was assessed using a Transwell migration assay. Cell invasion was measured at 22 h using a Matrigel assay. Cell adhesion was assessed at 90 min with a Cytoselect Adhesion assay. The two Kava preparations contained substantially different concentrations of the main chemical constituents. Treatment of malignant and normal oral keratinocyte cell lines with three of the identified constituents, 10 μg/ml FKA, 2.5 μg/ml FKB and 10 μg/ml yangonin, showed a significant reduction in cell proliferation in both H400 and BICR56 cancer cell lines but not in normal OKF6 cells. Remarkably, the same Kava constituents induced a significant reduction of OSCC cell migration and invasion. We have demonstrated, for the first time, that Kava constituents, FKA, FKB and yangonin have potential anticancer effects on OSCC. This highlights an avenue for further research of Kava constituents in the development of future cancer therapies to prevent and treat OSCC.
format	article
author	Antonio Celentano Callisthenis Yiannis Rita Paolini Pangzhen Zhang Camile S. Farah Nicola Cirillo Tami Yap Michael McCullough
author_facet	Antonio Celentano Callisthenis Yiannis Rita Paolini Pangzhen Zhang Camile S. Farah Nicola Cirillo Tami Yap Michael McCullough
author_sort	Antonio Celentano
title	Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
title_short	Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
title_full	Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
title_fullStr	Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
title_full_unstemmed	Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
title_sort	kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2d9f9316fc9c4a0e93d2b5b6c43e8848
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Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro

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