Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout.
Teleost fish express highly diverse naive TCRβ (TRB) repertoires and mount strong public and private clonal responses upon infection with pathogens. Fish T cells express typical markers such as CD8, CD4-1 and CD4-2, CD3, CD28 and CTLA4. Fish CD8(+) T cells have been shown to be responsible for antig...
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2013
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oai:doaj.org-article:2da93e94442d4a80a7921a7214145dcd2021-11-18T07:51:02ZContrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout.1932-620310.1371/journal.pone.0060175https://doaj.org/article/2da93e94442d4a80a7921a7214145dcd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23565199/?tool=EBIhttps://doaj.org/toc/1932-6203Teleost fish express highly diverse naive TCRβ (TRB) repertoires and mount strong public and private clonal responses upon infection with pathogens. Fish T cells express typical markers such as CD8, CD4-1 and CD4-2, CD3, CD28 and CTLA4. Fish CD8(+) T cells have been shown to be responsible for antigen-specific cell-mediated cytotoxicity in in vitro systems using histo-compatible effector and target cells. We compare here the complexity of TRB repertoires between FACS sorted CD8(+) and CD8(-) T cells from spleen and pronephros of rainbow trout. In contrast to human, while the TRB repertoire is highly diverse and polyclonal in CD8(+) T cells of naïve fish, it appeared very different in CD8(-) lymphocytes with irregular CDR3 length distributions suggesting a dominance of activated clones already in naïve fish or the presence of non conventional T cells. After infection with a systemic virus, CD8(+) T cells mount a typical response with significant skewing of CDR3 length profiles. The infection also induces significant modifications of the TRB repertoire expressed by the CD8(-) fraction, but for a different set of V/J combinations. In this fraction, the antiviral response results in an increase of the peak diversity of spectratypes. This unusual observation reflects the presence of a number of T cell expansions that rise the relative importance of minor peaks of the highly skewed distributions observed in unchallenged animals. These results suggest that the diversity of TRB expressed by CD8(+) and CD8(-) αβ T cells may be subjected to different regulatory patterns in fish and in mammals.Rosario CastroFumio TakizawaWahiba ChaaraAurélie LunazziThi Huong DangBernd KoellnerEdwige QuilletAdrien SixUwe FischerPierre BoudinotPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60175 (2013) |
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Medicine R Science Q Rosario Castro Fumio Takizawa Wahiba Chaara Aurélie Lunazzi Thi Huong Dang Bernd Koellner Edwige Quillet Adrien Six Uwe Fischer Pierre Boudinot Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout. |
| description |
Teleost fish express highly diverse naive TCRβ (TRB) repertoires and mount strong public and private clonal responses upon infection with pathogens. Fish T cells express typical markers such as CD8, CD4-1 and CD4-2, CD3, CD28 and CTLA4. Fish CD8(+) T cells have been shown to be responsible for antigen-specific cell-mediated cytotoxicity in in vitro systems using histo-compatible effector and target cells. We compare here the complexity of TRB repertoires between FACS sorted CD8(+) and CD8(-) T cells from spleen and pronephros of rainbow trout. In contrast to human, while the TRB repertoire is highly diverse and polyclonal in CD8(+) T cells of naïve fish, it appeared very different in CD8(-) lymphocytes with irregular CDR3 length distributions suggesting a dominance of activated clones already in naïve fish or the presence of non conventional T cells. After infection with a systemic virus, CD8(+) T cells mount a typical response with significant skewing of CDR3 length profiles. The infection also induces significant modifications of the TRB repertoire expressed by the CD8(-) fraction, but for a different set of V/J combinations. In this fraction, the antiviral response results in an increase of the peak diversity of spectratypes. This unusual observation reflects the presence of a number of T cell expansions that rise the relative importance of minor peaks of the highly skewed distributions observed in unchallenged animals. These results suggest that the diversity of TRB expressed by CD8(+) and CD8(-) αβ T cells may be subjected to different regulatory patterns in fish and in mammals. |
| format |
article |
| author |
Rosario Castro Fumio Takizawa Wahiba Chaara Aurélie Lunazzi Thi Huong Dang Bernd Koellner Edwige Quillet Adrien Six Uwe Fischer Pierre Boudinot |
| author_facet |
Rosario Castro Fumio Takizawa Wahiba Chaara Aurélie Lunazzi Thi Huong Dang Bernd Koellner Edwige Quillet Adrien Six Uwe Fischer Pierre Boudinot |
| author_sort |
Rosario Castro |
| title |
Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout. |
| title_short |
Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout. |
| title_full |
Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout. |
| title_fullStr |
Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout. |
| title_full_unstemmed |
Contrasted TCRβ diversity of CD8+ and CD8- T cells in rainbow trout. |
| title_sort |
contrasted tcrβ diversity of cd8+ and cd8- t cells in rainbow trout. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/2da93e94442d4a80a7921a7214145dcd |
| work_keys_str_mv |
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| _version_ |
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