The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions

Abstract Heat shock is known to accelerate mitochondrial ROS production in Saccharomyces cerevisiae cells. But how yeast mitochondria produce ROS under heat-shock condition is not completely clear. Previously, it was shown that ROS production in heat-stressed fermenting yeast cells was accompanied b...

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Autores principales:	Irina V. Fedoseeva, Darya V. Pyatrikas, Alexei V. Stepanov, Anna V. Fedyaeva, Nina N. Varakina, Tatyana M. Rusaleva, Gennadii B. Borovskii, Eugene G. Rikhvanov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2daf7dfd14ed45be8ea8ee582abd8cdf
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spelling	oai:doaj.org-article:2daf7dfd14ed45be8ea8ee582abd8cdf2021-12-02T15:04:51ZThe role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions10.1038/s41598-017-02736-72045-2322https://doaj.org/article/2daf7dfd14ed45be8ea8ee582abd8cdf2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02736-7https://doaj.org/toc/2045-2322Abstract Heat shock is known to accelerate mitochondrial ROS production in Saccharomyces cerevisiae cells. But how yeast mitochondria produce ROS under heat-shock condition is not completely clear. Previously, it was shown that ROS production in heat-stressed fermenting yeast cells was accompanied by mitochondrial membrane potential (MMP) increase. In the current investigation the relationship between ROS production and MMP was studied in respiring yeast cells in stationary phase, using diphenyleneiodonium chloride (DPI), an inhibitor of flavin-containing proteins, as well as the mutants deleted for NDE1, NDE2 and NDI1 genes, encoding flavin-containing external and internal NADH dehydrogenases. It was shown that heat shock induced a transient burst in mitochondrial ROS production, which was paralleled by MMP rise. ROS production and MMP was significantly suppressed by DPI addition and deletion of NDE1. The effect of DPI on ROS production and MMP rise was specific for respiring cells. The results obtained suggest that the functioning of mitochondrial flavin-binding enzymes, Nde1p for instance, is required for the hyperpolarization of inner mitochondrial membrane and ROS production in respiring S. cerevisiae cells under heat-shock conditions.Irina V. FedoseevaDarya V. PyatrikasAlexei V. StepanovAnna V. FedyaevaNina N. VarakinaTatyana M. RusalevaGennadii B. BorovskiiEugene G. RikhvanovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irina V. Fedoseeva Darya V. Pyatrikas Alexei V. Stepanov Anna V. Fedyaeva Nina N. Varakina Tatyana M. Rusaleva Gennadii B. Borovskii Eugene G. Rikhvanov The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions
description	Abstract Heat shock is known to accelerate mitochondrial ROS production in Saccharomyces cerevisiae cells. But how yeast mitochondria produce ROS under heat-shock condition is not completely clear. Previously, it was shown that ROS production in heat-stressed fermenting yeast cells was accompanied by mitochondrial membrane potential (MMP) increase. In the current investigation the relationship between ROS production and MMP was studied in respiring yeast cells in stationary phase, using diphenyleneiodonium chloride (DPI), an inhibitor of flavin-containing proteins, as well as the mutants deleted for NDE1, NDE2 and NDI1 genes, encoding flavin-containing external and internal NADH dehydrogenases. It was shown that heat shock induced a transient burst in mitochondrial ROS production, which was paralleled by MMP rise. ROS production and MMP was significantly suppressed by DPI addition and deletion of NDE1. The effect of DPI on ROS production and MMP rise was specific for respiring cells. The results obtained suggest that the functioning of mitochondrial flavin-binding enzymes, Nde1p for instance, is required for the hyperpolarization of inner mitochondrial membrane and ROS production in respiring S. cerevisiae cells under heat-shock conditions.
format	article
author	Irina V. Fedoseeva Darya V. Pyatrikas Alexei V. Stepanov Anna V. Fedyaeva Nina N. Varakina Tatyana M. Rusaleva Gennadii B. Borovskii Eugene G. Rikhvanov
author_facet	Irina V. Fedoseeva Darya V. Pyatrikas Alexei V. Stepanov Anna V. Fedyaeva Nina N. Varakina Tatyana M. Rusaleva Gennadii B. Borovskii Eugene G. Rikhvanov
author_sort	Irina V. Fedoseeva
title	The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions
title_short	The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions
title_full	The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions
title_fullStr	The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions
title_full_unstemmed	The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions
title_sort	role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ros production in respiring saccharomyces cerevisiae cells under heat-shock conditions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2daf7dfd14ed45be8ea8ee582abd8cdf
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The role of flavin-containing enzymes in mitochondrial membrane hyperpolarization and ROS production in respiring Saccharomyces cerevisiae cells under heat-shock conditions

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