Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.

We have previously shown that the respiratory syncytial virus [RSV] can productively infect monocyte derived dendritic cells [MoDC] and remain dormant within the same cells for prolonged periods. It is therefore possible that infected dendritic cells act as a reservoir within the airways of individu...

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Autores principales:	Kelechi Ugonna, Colin D Bingle, Karen Plant, Kirsty Wilson, Mark L Everard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2db8956e07494943a59584dad6a64dc8
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spelling	oai:doaj.org-article:2db8956e07494943a59584dad6a64dc82021-11-18T08:27:10ZMacrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.1932-620310.1371/journal.pone.0091855https://doaj.org/article/2db8956e07494943a59584dad6a64dc82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24651119/?tool=EBIhttps://doaj.org/toc/1932-6203We have previously shown that the respiratory syncytial virus [RSV] can productively infect monocyte derived dendritic cells [MoDC] and remain dormant within the same cells for prolonged periods. It is therefore possible that infected dendritic cells act as a reservoir within the airways of individuals between annual epidemics. In the present study we explored the possibility that sub-epithelial DCs can be infected with RSV from differentiated bronchial epithelium and that in turn RSV from DCs can infect the epithelium. A dual co-culture model was established in which a differentiated primary airway epithelium on an Air Liquid Interface (ALI) was cultured on a transwell insert and MoDCs were subsequently added to the basolateral membrane of the insert. Further experiments were undertaken using a triple co-culture model in which in which macrophages were added to the apical surface of the differentiated epithelium. A modified RSV [rr-RSV] expressing a red fluorescent protein marker of replication was used to infect either the MoDCs or the differentiated epithelium and infection of the reciprocal cell type was assessed using confocal microscopy. Our data shows that primary epithelium became infected when rr-RSV infected MoDCs were introduced onto the basal surface of the transwell insert. MoDCs located beneath the epithelium did not become infected with virus from infected epithelial cells in the dual co-culture model. However when macrophages were present on the apical surface of the primary epithelium infection of the basal MoDCs occurred. Our data suggests that RSV infected dendritic cells readily transmit infection to epithelial cells even when they are located beneath the basal layer. However macrophages appear to be necessary for the transmission of infection from epithelial cells to basal dendritic cells.Kelechi UgonnaColin D BingleKaren PlantKirsty WilsonMark L EverardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91855 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kelechi Ugonna Colin D Bingle Karen Plant Kirsty Wilson Mark L Everard Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
description	We have previously shown that the respiratory syncytial virus [RSV] can productively infect monocyte derived dendritic cells [MoDC] and remain dormant within the same cells for prolonged periods. It is therefore possible that infected dendritic cells act as a reservoir within the airways of individuals between annual epidemics. In the present study we explored the possibility that sub-epithelial DCs can be infected with RSV from differentiated bronchial epithelium and that in turn RSV from DCs can infect the epithelium. A dual co-culture model was established in which a differentiated primary airway epithelium on an Air Liquid Interface (ALI) was cultured on a transwell insert and MoDCs were subsequently added to the basolateral membrane of the insert. Further experiments were undertaken using a triple co-culture model in which in which macrophages were added to the apical surface of the differentiated epithelium. A modified RSV [rr-RSV] expressing a red fluorescent protein marker of replication was used to infect either the MoDCs or the differentiated epithelium and infection of the reciprocal cell type was assessed using confocal microscopy. Our data shows that primary epithelium became infected when rr-RSV infected MoDCs were introduced onto the basal surface of the transwell insert. MoDCs located beneath the epithelium did not become infected with virus from infected epithelial cells in the dual co-culture model. However when macrophages were present on the apical surface of the primary epithelium infection of the basal MoDCs occurred. Our data suggests that RSV infected dendritic cells readily transmit infection to epithelial cells even when they are located beneath the basal layer. However macrophages appear to be necessary for the transmission of infection from epithelial cells to basal dendritic cells.
format	article
author	Kelechi Ugonna Colin D Bingle Karen Plant Kirsty Wilson Mark L Everard
author_facet	Kelechi Ugonna Colin D Bingle Karen Plant Kirsty Wilson Mark L Everard
author_sort	Kelechi Ugonna
title	Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
title_short	Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
title_full	Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
title_fullStr	Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
title_full_unstemmed	Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
title_sort	macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2db8956e07494943a59584dad6a64dc8
work_keys_str_mv	AT kelechiugonna macrophagesarerequiredfordendriticcelluptakeofrespiratorysyncytialvirusfromaninfectedepithelium AT colindbingle macrophagesarerequiredfordendriticcelluptakeofrespiratorysyncytialvirusfromaninfectedepithelium AT karenplant macrophagesarerequiredfordendriticcelluptakeofrespiratorysyncytialvirusfromaninfectedepithelium AT kirstywilson macrophagesarerequiredfordendriticcelluptakeofrespiratorysyncytialvirusfromaninfectedepithelium AT markleverard macrophagesarerequiredfordendriticcelluptakeofrespiratorysyncytialvirusfromaninfectedepithelium
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Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.

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