Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate

Objective To explore effect on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan (CMC)/amorphous calcium phosphate (ACP). Methods Remineralized solultion at different stages were prepared: ①reactive CMC/ACP (CMC/ACP nanoparticles treated with NaClO), ②r...

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Autores principales: GUO Honglei, ZHANG Kai, ZHANG Xu
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Publicado: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2022
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spelling oai:doaj.org-article:2dc46b60f15341d0bb57b1d45cf658762021-11-25T06:45:57ZStudy on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate10.12016/j.issn.2096-1456.2022.02.0022096-1456https://doaj.org/article/2dc46b60f15341d0bb57b1d45cf658762022-02-01T00:00:00Zhttp://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2022.02.002https://doaj.org/toc/2096-1456Objective To explore effect on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan (CMC)/amorphous calcium phosphate (ACP). Methods Remineralized solultion at different stages were prepared: ①reactive CMC/ACP (CMC/ACP nanoparticles treated with NaClO), ②reactive CMC/ACP+glycine; transmission electron microscopy was used to detect the morphology of the remineralized solution particles. Twenty teeth were randomly divided into two groups: group A and group B. Reactive CMC/ACP was applied to the enamel surface of group A and group B was treated with reactive CMC/ACP remineralization solution containing glycine. Scanning electron microscopy was used to detect the enamel surface morphology before and after remineralization, and nanoindentation was used to detect the mechanical strength (including nanoindentation depth, hardness and elastic modulus) of the enamel surface. Results Under a transmission electron microscope, the particles in the reactive CMC/ACP remineralization solution were smooth, and the increase in particle size was approximately 100-300 nm. After the addition of glycine, the particles in the reactive CMC/ACP remineralization solution particles showed a linear ordered arrangement, and microcrystals were formed in the solution 15 min later, with a crystal length of approximately 5-15 μm. Remineralization in group A was granular and heterogeneous. In group B, the crystal morphology of the demineralized enamel was homogeneous and ordered, similar to that of natural enamel. The nanoindentation depth of group B after remineralization was smaller than that of group A, and it was closest to that of natural enamel, there was no significant difference between group B and natural enamel in terms of the hardness and elastic modulus of the enamel surface after remineralization. Conclusion CMC/ACP nanoparticles treated with NaClO can rapidly and specifically form directional and ordered remineralization on the enamel surface of a model of glycine-guided rapid remineralization of enamel caries. The surface structure of remineralized enamel is similar to that of natural enamel in terms of nanoindentation depth, hardness and elastic modulus.GUO HongleiZHANG KaiZHANG XuEditorial Department of Journal of Prevention and Treatment for Stomatological Diseasesarticlehydroxyapatiteenamel cariesremineralizationcarboxymethyl chitosanamorphous calcium phosphatenacloglycinenanoindenterMedicineRZH口腔疾病防治, Vol 30, Iss 2, Pp 83-88 (2022)
institution DOAJ
collection DOAJ
language ZH
topic hydroxyapatite
enamel caries
remineralization
carboxymethyl chitosan
amorphous calcium phosphate
naclo
glycine
nanoindenter
Medicine
R
spellingShingle hydroxyapatite
enamel caries
remineralization
carboxymethyl chitosan
amorphous calcium phosphate
naclo
glycine
nanoindenter
Medicine
R
GUO Honglei
ZHANG Kai
ZHANG Xu
Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
description Objective To explore effect on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan (CMC)/amorphous calcium phosphate (ACP). Methods Remineralized solultion at different stages were prepared: ①reactive CMC/ACP (CMC/ACP nanoparticles treated with NaClO), ②reactive CMC/ACP+glycine; transmission electron microscopy was used to detect the morphology of the remineralized solution particles. Twenty teeth were randomly divided into two groups: group A and group B. Reactive CMC/ACP was applied to the enamel surface of group A and group B was treated with reactive CMC/ACP remineralization solution containing glycine. Scanning electron microscopy was used to detect the enamel surface morphology before and after remineralization, and nanoindentation was used to detect the mechanical strength (including nanoindentation depth, hardness and elastic modulus) of the enamel surface. Results Under a transmission electron microscope, the particles in the reactive CMC/ACP remineralization solution were smooth, and the increase in particle size was approximately 100-300 nm. After the addition of glycine, the particles in the reactive CMC/ACP remineralization solution particles showed a linear ordered arrangement, and microcrystals were formed in the solution 15 min later, with a crystal length of approximately 5-15 μm. Remineralization in group A was granular and heterogeneous. In group B, the crystal morphology of the demineralized enamel was homogeneous and ordered, similar to that of natural enamel. The nanoindentation depth of group B after remineralization was smaller than that of group A, and it was closest to that of natural enamel, there was no significant difference between group B and natural enamel in terms of the hardness and elastic modulus of the enamel surface after remineralization. Conclusion CMC/ACP nanoparticles treated with NaClO can rapidly and specifically form directional and ordered remineralization on the enamel surface of a model of glycine-guided rapid remineralization of enamel caries. The surface structure of remineralized enamel is similar to that of natural enamel in terms of nanoindentation depth, hardness and elastic modulus.
format article
author GUO Honglei
ZHANG Kai
ZHANG Xu
author_facet GUO Honglei
ZHANG Kai
ZHANG Xu
author_sort GUO Honglei
title Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
title_short Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
title_full Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
title_fullStr Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
title_full_unstemmed Study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
title_sort study on the remineralization of demineralized enamel surfaces with glycine-guided carboxymethyl chitosan/amorphous calcium phosphate
publisher Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
publishDate 2022
url https://doaj.org/article/2dc46b60f15341d0bb57b1d45cf65876
work_keys_str_mv AT guohonglei studyontheremineralizationofdemineralizedenamelsurfaceswithglycineguidedcarboxymethylchitosanamorphouscalciumphosphate
AT zhangkai studyontheremineralizationofdemineralizedenamelsurfaceswithglycineguidedcarboxymethylchitosanamorphouscalciumphosphate
AT zhangxu studyontheremineralizationofdemineralizedenamelsurfaceswithglycineguidedcarboxymethylchitosanamorphouscalciumphosphate
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