Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.

Members of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, V. harveyi and V. vulnificus. We have used transposon mutagenesis to ident...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pimonsri Rattanama, Janelle R Thompson, Natthawan Kongkerd, Kanchana Srinitiwarawong, Varaporn Vuddhakul, John J Mekalanos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ddb806bae7d4a2faf62960313cbab2c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2ddb806bae7d4a2faf62960313cbab2c
record_format	dspace
spelling	oai:doaj.org-article:2ddb806bae7d4a2faf62960313cbab2c2021-11-18T07:26:50ZSigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.1932-620310.1371/journal.pone.0032523https://doaj.org/article/2ddb806bae7d4a2faf62960313cbab2c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22384269/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Members of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, V. harveyi and V. vulnificus. We have used transposon mutagenesis to identify genes involved in the hemolytic activity of shrimp-pathogenic V. harveyi strain PSU3316. Out of 1,764 mutants screened, five mutants showed reduced hemolytic activity on sheep blood agar and exhibited virulence attenuation in shrimp (Litopenaeus vannamei). Mutants were identified by comparing transposon junction sequences to a draft of assembly of the PSU3316 genome. Surprisingly none of the disrupted open reading frames or gene neighborhoods contained genes annotated as hemolysins. The gene encoding RseB, a negative regulator of the sigma factor (σ(E)), was interrupted in 2 out of 5 transposon mutants, in addition, the transcription factor CytR, a threonine synthetase, and an efflux-associated cytoplasmic protein were also identified. Knockout mutations introduced into the rpoE operon at the rseB gene exhibited low hemolytic activity in sheep blood agar, and were 3-to 7-fold attenuated for colonization in shrimp. Comparison of whole cell extracted proteins in the rseB mutant (PSU4030) to the wild-type by 2-D gel electrophoresis revealed 6 differentially expressed proteins, including two down-regulated porins (OmpC-like and OmpN) and an upregulated protease (DegQ) which have been associated with σ(E) in other organisms. Our study is the first report linking hemolytic activity to the σ(E) regulators in pathogenic Vibrio species and suggests expression of this virulence-linked phenotype is governed by multiple regulatory pathways within the V. harveyi.Pimonsri RattanamaJanelle R ThompsonNatthawan KongkerdKanchana SrinitiwarawongVaraporn VuddhakulJohn J MekalanosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e32523 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pimonsri Rattanama Janelle R Thompson Natthawan Kongkerd Kanchana Srinitiwarawong Varaporn Vuddhakul John J Mekalanos Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
description	Members of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, V. harveyi and V. vulnificus. We have used transposon mutagenesis to identify genes involved in the hemolytic activity of shrimp-pathogenic V. harveyi strain PSU3316. Out of 1,764 mutants screened, five mutants showed reduced hemolytic activity on sheep blood agar and exhibited virulence attenuation in shrimp (Litopenaeus vannamei). Mutants were identified by comparing transposon junction sequences to a draft of assembly of the PSU3316 genome. Surprisingly none of the disrupted open reading frames or gene neighborhoods contained genes annotated as hemolysins. The gene encoding RseB, a negative regulator of the sigma factor (σ(E)), was interrupted in 2 out of 5 transposon mutants, in addition, the transcription factor CytR, a threonine synthetase, and an efflux-associated cytoplasmic protein were also identified. Knockout mutations introduced into the rpoE operon at the rseB gene exhibited low hemolytic activity in sheep blood agar, and were 3-to 7-fold attenuated for colonization in shrimp. Comparison of whole cell extracted proteins in the rseB mutant (PSU4030) to the wild-type by 2-D gel electrophoresis revealed 6 differentially expressed proteins, including two down-regulated porins (OmpC-like and OmpN) and an upregulated protease (DegQ) which have been associated with σ(E) in other organisms. Our study is the first report linking hemolytic activity to the σ(E) regulators in pathogenic Vibrio species and suggests expression of this virulence-linked phenotype is governed by multiple regulatory pathways within the V. harveyi.
format	article
author	Pimonsri Rattanama Janelle R Thompson Natthawan Kongkerd Kanchana Srinitiwarawong Varaporn Vuddhakul John J Mekalanos
author_facet	Pimonsri Rattanama Janelle R Thompson Natthawan Kongkerd Kanchana Srinitiwarawong Varaporn Vuddhakul John J Mekalanos
author_sort	Pimonsri Rattanama
title	Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
title_short	Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
title_full	Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
title_fullStr	Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
title_full_unstemmed	Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
title_sort	sigma e regulators control hemolytic activity and virulence in a shrimp pathogenic vibrio harveyi.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2ddb806bae7d4a2faf62960313cbab2c
work_keys_str_mv	AT pimonsrirattanama sigmaeregulatorscontrolhemolyticactivityandvirulenceinashrimppathogenicvibrioharveyi AT janellerthompson sigmaeregulatorscontrolhemolyticactivityandvirulenceinashrimppathogenicvibrioharveyi AT natthawankongkerd sigmaeregulatorscontrolhemolyticactivityandvirulenceinashrimppathogenicvibrioharveyi AT kanchanasrinitiwarawong sigmaeregulatorscontrolhemolyticactivityandvirulenceinashrimppathogenicvibrioharveyi AT varapornvuddhakul sigmaeregulatorscontrolhemolyticactivityandvirulenceinashrimppathogenicvibrioharveyi AT johnjmekalanos sigmaeregulatorscontrolhemolyticactivityandvirulenceinashrimppathogenicvibrioharveyi
_version_	1718423398528319488

Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.

Ejemplares similares