Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources

ABSTRACT Pseudomonas aeruginosa is an opportunistic human pathogen, particularly noted for causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have shown that the gene expression profile of P. aeruginosa appears to converge toward a common metabolic program as the o...

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Autores principales:	Stephen K. Dolan, Michael Kohlstedt, Stephen Trigg, Pedro Vallejo Ramirez, Clemens F. Kaminski, Christoph Wittmann, Martin Welch
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Pseudomonas aeruginosa carbon metabolism denitrification proteomics carbon flux acetate metabolism Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2ddf3bc86caa447c8bf3811811603985
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spelling	oai:doaj.org-article:2ddf3bc86caa447c8bf38118116039852021-11-15T15:57:03ZContextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources10.1128/mBio.02684-192150-7511https://doaj.org/article/2ddf3bc86caa447c8bf38118116039852020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02684-19https://doaj.org/toc/2150-7511ABSTRACT Pseudomonas aeruginosa is an opportunistic human pathogen, particularly noted for causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have shown that the gene expression profile of P. aeruginosa appears to converge toward a common metabolic program as the organism adapts to the CF airway environment. However, we still have only a limited understanding of how these transcriptional changes impact metabolic flux at the systems level. To address this, we analyzed the transcriptome, proteome, and fluxome of P. aeruginosa grown on glycerol or acetate. These carbon sources were chosen because they are the primary breakdown products of an airway surfactant, phosphatidylcholine, which is known to be a major carbon source for P. aeruginosa in CF airways. We show that the fluxes of carbon throughout central metabolism are radically different among carbon sources. For example, the newly recognized “EDEMP cycle” (which incorporates elements of the Entner-Doudoroff [ED] pathway, the Embden-Meyerhof-Parnas [EMP] pathway, and the pentose phosphate [PP] pathway) plays an important role in supplying NADPH during growth on glycerol. In contrast, the EDEMP cycle is attenuated during growth on acetate, and instead, NADPH is primarily supplied by the reaction catalyzed by isocitrate dehydrogenase(s). Perhaps more importantly, our proteomic and transcriptomic analyses revealed a global remodeling of gene expression during growth on the different carbon sources, with unanticipated impacts on aerobic denitrification, electron transport chain architecture, and the redox economy of the cell. Collectively, these data highlight the remarkable metabolic plasticity of P. aeruginosa; that plasticity allows the organism to seamlessly segue between different carbon sources, maximizing the energetic yield from each. IMPORTANCE Pseudomonas aeruginosa is an opportunistic human pathogen that is well known for causing infections in the airways of people with cystic fibrosis. Although it is clear that P. aeruginosa is metabolically well adapted to life in the CF lung, little is currently known about how the organism metabolizes the nutrients available in the airways. In this work, we used a combination of gene expression and isotope tracer (“fluxomic”) analyses to find out exactly where the input carbon goes during growth on two CF-relevant carbon sources, acetate and glycerol (derived from the breakdown of lung surfactant). We found that carbon is routed (“fluxed”) through very different pathways during growth on these substrates and that this is accompanied by an unexpected remodeling of the cell’s electron transfer pathways. Having access to this “blueprint” is important because the metabolism of P. aeruginosa is increasingly being recognized as a target for the development of much-needed antimicrobial agents.Stephen K. DolanMichael KohlstedtStephen TriggPedro Vallejo RamirezClemens F. KaminskiChristoph WittmannMartin WelchAmerican Society for MicrobiologyarticlePseudomonas aeruginosacarbon metabolismdenitrificationproteomicscarbon fluxacetate metabolismMicrobiologyQR1-502ENmBio, Vol 11, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas aeruginosa carbon metabolism denitrification proteomics carbon flux acetate metabolism Microbiology QR1-502
spellingShingle	Pseudomonas aeruginosa carbon metabolism denitrification proteomics carbon flux acetate metabolism Microbiology QR1-502 Stephen K. Dolan Michael Kohlstedt Stephen Trigg Pedro Vallejo Ramirez Clemens F. Kaminski Christoph Wittmann Martin Welch Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources
description	ABSTRACT Pseudomonas aeruginosa is an opportunistic human pathogen, particularly noted for causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have shown that the gene expression profile of P. aeruginosa appears to converge toward a common metabolic program as the organism adapts to the CF airway environment. However, we still have only a limited understanding of how these transcriptional changes impact metabolic flux at the systems level. To address this, we analyzed the transcriptome, proteome, and fluxome of P. aeruginosa grown on glycerol or acetate. These carbon sources were chosen because they are the primary breakdown products of an airway surfactant, phosphatidylcholine, which is known to be a major carbon source for P. aeruginosa in CF airways. We show that the fluxes of carbon throughout central metabolism are radically different among carbon sources. For example, the newly recognized “EDEMP cycle” (which incorporates elements of the Entner-Doudoroff [ED] pathway, the Embden-Meyerhof-Parnas [EMP] pathway, and the pentose phosphate [PP] pathway) plays an important role in supplying NADPH during growth on glycerol. In contrast, the EDEMP cycle is attenuated during growth on acetate, and instead, NADPH is primarily supplied by the reaction catalyzed by isocitrate dehydrogenase(s). Perhaps more importantly, our proteomic and transcriptomic analyses revealed a global remodeling of gene expression during growth on the different carbon sources, with unanticipated impacts on aerobic denitrification, electron transport chain architecture, and the redox economy of the cell. Collectively, these data highlight the remarkable metabolic plasticity of P. aeruginosa; that plasticity allows the organism to seamlessly segue between different carbon sources, maximizing the energetic yield from each. IMPORTANCE Pseudomonas aeruginosa is an opportunistic human pathogen that is well known for causing infections in the airways of people with cystic fibrosis. Although it is clear that P. aeruginosa is metabolically well adapted to life in the CF lung, little is currently known about how the organism metabolizes the nutrients available in the airways. In this work, we used a combination of gene expression and isotope tracer (“fluxomic”) analyses to find out exactly where the input carbon goes during growth on two CF-relevant carbon sources, acetate and glycerol (derived from the breakdown of lung surfactant). We found that carbon is routed (“fluxed”) through very different pathways during growth on these substrates and that this is accompanied by an unexpected remodeling of the cell’s electron transfer pathways. Having access to this “blueprint” is important because the metabolism of P. aeruginosa is increasingly being recognized as a target for the development of much-needed antimicrobial agents.
format	article
author	Stephen K. Dolan Michael Kohlstedt Stephen Trigg Pedro Vallejo Ramirez Clemens F. Kaminski Christoph Wittmann Martin Welch
author_facet	Stephen K. Dolan Michael Kohlstedt Stephen Trigg Pedro Vallejo Ramirez Clemens F. Kaminski Christoph Wittmann Martin Welch
author_sort	Stephen K. Dolan
title	Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources
title_short	Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources
title_full	Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources
title_fullStr	Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources
title_full_unstemmed	Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources
title_sort	contextual flexibility in <named-content content-type="genus-species">pseudomonas aeruginosa</named-content> central carbon metabolism during growth in single carbon sources
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/2ddf3bc86caa447c8bf3811811603985
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Contextual Flexibility in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Central Carbon Metabolism during Growth in Single Carbon Sources

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