Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice

Nicotine withdrawal symptoms, mainly anxiety, cause high level of relapse rate after quitting smoking. Vitamin D supplementation has shown its potential for the prevention and treatment of anxiety disorders; however, neurobiological studies about the effect of vitamin D on nicotine withdrawal-induce...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Wu Bingxue, Tao Xinrong, Liu Chuanlin, Li Huaixu, Jiang Tao, Chen Zijun, Wang Qi, Liu Fei, Mu Min, Chen Zhaoyan
Formato: article
Lenguaje:EN
Publicado: De Gruyter 2021
Materias:
Acceso en línea:https://doaj.org/article/2de0c3b25e73436993231c99deb9847a
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:2de0c3b25e73436993231c99deb9847a
record_format dspace
spelling oai:doaj.org-article:2de0c3b25e73436993231c99deb9847a2021-12-05T14:11:05ZVitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice2081-693610.1515/tnsci-2020-0166https://doaj.org/article/2de0c3b25e73436993231c99deb9847a2021-06-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2020-0166https://doaj.org/toc/2081-6936Nicotine withdrawal symptoms, mainly anxiety, cause high level of relapse rate after quitting smoking. Vitamin D supplementation has shown its potential for the prevention and treatment of anxiety disorders; however, neurobiological studies about the effect of vitamin D on nicotine withdrawal-induced anxiety are limited. To investigate the effect and molecular mechanism of vitamin D3 supplement by dietary on anxiety-like behavior during nicotine withdrawal, male C57/BL6 mice were divided into four groups: vehicle, nicotine only, vitamin D3 only, and nicotine plus vitamin D3. Mice were administrated with nicotine in drinking water (200 µg/mL), and vitamin D3 in feed for 6 weeks. During nicotine withdrawal, vitamin D3-treated mice showed significantly less anxiety-like behavior by an open-field test and marble buried test that performed an increase in the duration of the central zone and a decrease buried marble, respectively. Moreover, vitamin D3 supplementation attenuated the hippocampal NR2A expression on both protein and mRNA levels in nicotine and vitamin D3-treated mice. Our data showed that dietary supplementation with vitamin D3 ameliorated nicotine withdrawal-induced anxiety, which may be related to downregulation of NR2A expression in hippocampus. Vitamin D3 may provide a new dietary intervention with the easy access for smoking cessation.Wu BingxueTao XinrongLiu ChuanlinLi HuaixuJiang TaoChen ZijunWang QiLiu FeiMu MinChen ZhaoyanDe Gruyterarticlenicotine withdrawalanxiety-like behaviorvitamin d3nr2ahippocampusNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 273-281 (2021)
institution DOAJ
collection DOAJ
language EN
topic nicotine withdrawal
anxiety-like behavior
vitamin d3
nr2a
hippocampus
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
spellingShingle nicotine withdrawal
anxiety-like behavior
vitamin d3
nr2a
hippocampus
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Wu Bingxue
Tao Xinrong
Liu Chuanlin
Li Huaixu
Jiang Tao
Chen Zijun
Wang Qi
Liu Fei
Mu Min
Chen Zhaoyan
Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice
description Nicotine withdrawal symptoms, mainly anxiety, cause high level of relapse rate after quitting smoking. Vitamin D supplementation has shown its potential for the prevention and treatment of anxiety disorders; however, neurobiological studies about the effect of vitamin D on nicotine withdrawal-induced anxiety are limited. To investigate the effect and molecular mechanism of vitamin D3 supplement by dietary on anxiety-like behavior during nicotine withdrawal, male C57/BL6 mice were divided into four groups: vehicle, nicotine only, vitamin D3 only, and nicotine plus vitamin D3. Mice were administrated with nicotine in drinking water (200 µg/mL), and vitamin D3 in feed for 6 weeks. During nicotine withdrawal, vitamin D3-treated mice showed significantly less anxiety-like behavior by an open-field test and marble buried test that performed an increase in the duration of the central zone and a decrease buried marble, respectively. Moreover, vitamin D3 supplementation attenuated the hippocampal NR2A expression on both protein and mRNA levels in nicotine and vitamin D3-treated mice. Our data showed that dietary supplementation with vitamin D3 ameliorated nicotine withdrawal-induced anxiety, which may be related to downregulation of NR2A expression in hippocampus. Vitamin D3 may provide a new dietary intervention with the easy access for smoking cessation.
format article
author Wu Bingxue
Tao Xinrong
Liu Chuanlin
Li Huaixu
Jiang Tao
Chen Zijun
Wang Qi
Liu Fei
Mu Min
Chen Zhaoyan
author_facet Wu Bingxue
Tao Xinrong
Liu Chuanlin
Li Huaixu
Jiang Tao
Chen Zijun
Wang Qi
Liu Fei
Mu Min
Chen Zhaoyan
author_sort Wu Bingxue
title Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice
title_short Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice
title_full Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice
title_fullStr Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice
title_full_unstemmed Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice
title_sort vitamin d3 reduces hippocampal nr2a and anxiety in nicotine withdrawal mice
publisher De Gruyter
publishDate 2021
url https://doaj.org/article/2de0c3b25e73436993231c99deb9847a
work_keys_str_mv AT wubingxue vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT taoxinrong vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT liuchuanlin vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT lihuaixu vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT jiangtao vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT chenzijun vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT wangqi vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT liufei vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT mumin vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
AT chenzhaoyan vitamind3reduceshippocampalnr2aandanxietyinnicotinewithdrawalmice
_version_ 1718371411293110272