Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level

Parathyroid hormone receptors PTH1R and PTH2R are poorly studied in the pathology of the vertebral column and may be involved in both ectopic ossification of the vertebral ligaments and fibrous growth of the extracellular matrix, as well as in other unknown processes occurring in the pathological fo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	L. V. Rodionova, L. G. Samoilova, V. A. Sorokovikov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	stenosis of the spinal canal degenerative spine disorders gene expression ligamentum flavum th1r pth2r Science Q
Acceso en línea:	https://doaj.org/article/2deab98d254b4b409594eb37c5f9cf06
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2deab98d254b4b409594eb37c5f9cf06
record_format	dspace
spelling	oai:doaj.org-article:2deab98d254b4b409594eb37c5f9cf062021-11-23T06:14:45ZActivity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level2541-94202587-959610.29413/ABS.2020-5.6.13https://doaj.org/article/2deab98d254b4b409594eb37c5f9cf062021-01-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2503https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Parathyroid hormone receptors PTH1R and PTH2R are poorly studied in the pathology of the vertebral column and may be involved in both ectopic ossification of the vertebral ligaments and fibrous growth of the extracellular matrix, as well as in other unknown processes occurring in the pathological focus. Aim. To study the expression of genes encoding parathyroid hormone receptors in intraoperative Ligamentum flavum samples of patients with stenosis of the spinal canal and the lumbar dural sac of the spine and to assess the correlation with the expression of other genes active in connective tissue in conditions of developing pathology.Materials and methods. Studies of the Ligamentum flavum were performed in 33 patients (17 women, 16 men) with stenotic processes of the spinal canal and dural sac of the lumbar spine. RNA was isolated from the biopsies using Qiagen reagents (Germany). Then reverse transcription was performed. cDNA was used for setting up a polymerase chain reaction (Promega Reagents, USA) using a CFX96 thermal cycler (Biorad, USA) with specially selected specific primers.Results. The expression of genes encoding parathyroid hormone receptors PTH1R and PTH2R was detected and evaluated for the first time in intraoperative Ligamentum flavum samples of patients with spinal canal and dural sac stenosis, and their relationship with the activity of genes encoding estradiol receptors (ESR1 and ESR2) and FGFR3 was revealed. For the first time, common features of PTH1R and PTH2R gene expression, as well as their differences, were revealed. It is proved that the intensity of expression of parathyroid hormone receptors in intraoperative Ligamentum flavum biopsies does not depend on age, gender, or the presence of bone and cartilage nodes according to MRI results. The interdependence of differential changes in the expression of genes of the receptor apparatus involved in the metabolism of Ligamentum flavum tissues was revealed.L. V. RodionovaL. G. SamoilovaV. A. SorokovikovScientific Сentre for Family Health and Human Reproduction Problemsarticlestenosis of the spinal canaldegenerative spine disordersgene expressionligamentum flavumth1rpth2rScienceQRUActa Biomedica Scientifica, Vol 5, Iss 6, Pp 113-123 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	stenosis of the spinal canal degenerative spine disorders gene expression ligamentum flavum th1r pth2r Science Q
spellingShingle	stenosis of the spinal canal degenerative spine disorders gene expression ligamentum flavum th1r pth2r Science Q L. V. Rodionova L. G. Samoilova V. A. Sorokovikov Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level
description	Parathyroid hormone receptors PTH1R and PTH2R are poorly studied in the pathology of the vertebral column and may be involved in both ectopic ossification of the vertebral ligaments and fibrous growth of the extracellular matrix, as well as in other unknown processes occurring in the pathological focus. Aim. To study the expression of genes encoding parathyroid hormone receptors in intraoperative Ligamentum flavum samples of patients with stenosis of the spinal canal and the lumbar dural sac of the spine and to assess the correlation with the expression of other genes active in connective tissue in conditions of developing pathology.Materials and methods. Studies of the Ligamentum flavum were performed in 33 patients (17 women, 16 men) with stenotic processes of the spinal canal and dural sac of the lumbar spine. RNA was isolated from the biopsies using Qiagen reagents (Germany). Then reverse transcription was performed. cDNA was used for setting up a polymerase chain reaction (Promega Reagents, USA) using a CFX96 thermal cycler (Biorad, USA) with specially selected specific primers.Results. The expression of genes encoding parathyroid hormone receptors PTH1R and PTH2R was detected and evaluated for the first time in intraoperative Ligamentum flavum samples of patients with spinal canal and dural sac stenosis, and their relationship with the activity of genes encoding estradiol receptors (ESR1 and ESR2) and FGFR3 was revealed. For the first time, common features of PTH1R and PTH2R gene expression, as well as their differences, were revealed. It is proved that the intensity of expression of parathyroid hormone receptors in intraoperative Ligamentum flavum biopsies does not depend on age, gender, or the presence of bone and cartilage nodes according to MRI results. The interdependence of differential changes in the expression of genes of the receptor apparatus involved in the metabolism of Ligamentum flavum tissues was revealed.
format	article
author	L. V. Rodionova L. G. Samoilova V. A. Sorokovikov
author_facet	L. V. Rodionova L. G. Samoilova V. A. Sorokovikov
author_sort	L. V. Rodionova
title	Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level
title_short	Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level
title_full	Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level
title_fullStr	Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level
title_full_unstemmed	Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level
title_sort	activity of parathyroid hormone receptor genes in ligamentum flavum biopsies of patients with spinal canal and dural sac stenosis at the lumbar level
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/2deab98d254b4b409594eb37c5f9cf06
work_keys_str_mv	AT lvrodionova activityofparathyroidhormonereceptorgenesinligamentumflavumbiopsiesofpatientswithspinalcanalandduralsacstenosisatthelumbarlevel AT lgsamoilova activityofparathyroidhormonereceptorgenesinligamentumflavumbiopsiesofpatientswithspinalcanalandduralsacstenosisatthelumbarlevel AT vasorokovikov activityofparathyroidhormonereceptorgenesinligamentumflavumbiopsiesofpatientswithspinalcanalandduralsacstenosisatthelumbarlevel
_version_	1718416872913764352

Activity of Parathyroid Hormone Receptor Genes in Ligamentum Flavum Biopsies of Patients with Spinal Canal and Dural Sac Stenosis at the Lumbar Level

Ejemplares similares