Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
(1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium&l...
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/2df40ca8f1bb40489b6b8242648ba61a |
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| Sumario: | (1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium</i>, <i>Penicillium</i>, <i>Plenodomus</i> and <i>Phytophthora</i>; the effectiveness of the substance with the highest <i>in vitro</i> activity in preventing citrus and apple fruit rot incited by <i>P. digitatum</i> and <i>P. expansum</i>, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; <i>in vitro</i> preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on <i>Plenodomus tracheiphilus</i>, <i>C. gloeosporioides</i> and <i>Ph. nicotianae</i>; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested <i>in vivo</i> were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens. |
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