Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

(1) Background: This study was aimed at determining the in vitro inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera Alternaria, Colletotrichum, Fusarium&l...

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Autores principales:	Soumia El boumlasy, Federico La Spada, Nunzio Tuccitto, Giovanni Marletta, Carlos Luz Mínguez, Giuseppe Meca, Ermes Ivan Rovetto, Antonella Pane, Abderrahmane Debdoubi, Santa Olga Cacciola
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	metabolites phenolic compounds inhibitory effect citrus apple HPLC-ESI-MS-TOF Botany QK1-989
Acceso en línea:	https://doaj.org/article/2df40ca8f1bb40489b6b8242648ba61a
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spelling	oai:doaj.org-article:2df40ca8f1bb40489b6b8242648ba61a2021-11-25T18:46:42ZInhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens10.3390/plants101124522223-7747https://doaj.org/article/2df40ca8f1bb40489b6b8242648ba61a2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2452https://doaj.org/toc/2223-7747(1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium</i>, <i>Penicillium</i>, <i>Plenodomus</i> and <i>Phytophthora</i>; the effectiveness of the substance with the highest <i>in vitro</i> activity in preventing citrus and apple fruit rot incited by <i>P. digitatum</i> and <i>P. expansum</i>, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; <i>in vitro</i> preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on <i>Plenodomus tracheiphilus</i>, <i>C. gloeosporioides</i> and <i>Ph. nicotianae</i>; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested <i>in vivo</i> were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens.Soumia El boumlasyFederico La SpadaNunzio TuccittoGiovanni MarlettaCarlos Luz MínguezGiuseppe MecaErmes Ivan RovettoAntonella PaneAbderrahmane DebdoubiSanta Olga CacciolaMDPI AGarticlemetabolitesphenolic compoundsinhibitory effectcitrusappleHPLC-ESI-MS-TOFBotanyQK1-989ENPlants, Vol 10, Iss 2452, p 2452 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metabolites phenolic compounds inhibitory effect citrus apple HPLC-ESI-MS-TOF Botany QK1-989
spellingShingle	metabolites phenolic compounds inhibitory effect citrus apple HPLC-ESI-MS-TOF Botany QK1-989 Soumia El boumlasy Federico La Spada Nunzio Tuccitto Giovanni Marletta Carlos Luz Mínguez Giuseppe Meca Ermes Ivan Rovetto Antonella Pane Abderrahmane Debdoubi Santa Olga Cacciola Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
description	(1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium</i>, <i>Penicillium</i>, <i>Plenodomus</i> and <i>Phytophthora</i>; the effectiveness of the substance with the highest <i>in vitro</i> activity in preventing citrus and apple fruit rot incited by <i>P. digitatum</i> and <i>P. expansum</i>, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; <i>in vitro</i> preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on <i>Plenodomus tracheiphilus</i>, <i>C. gloeosporioides</i> and <i>Ph. nicotianae</i>; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested <i>in vivo</i> were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens.
format	article
author	Soumia El boumlasy Federico La Spada Nunzio Tuccitto Giovanni Marletta Carlos Luz Mínguez Giuseppe Meca Ermes Ivan Rovetto Antonella Pane Abderrahmane Debdoubi Santa Olga Cacciola
author_facet	Soumia El boumlasy Federico La Spada Nunzio Tuccitto Giovanni Marletta Carlos Luz Mínguez Giuseppe Meca Ermes Ivan Rovetto Antonella Pane Abderrahmane Debdoubi Santa Olga Cacciola
author_sort	Soumia El boumlasy
title	Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
title_short	Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
title_full	Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
title_fullStr	Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
title_full_unstemmed	Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
title_sort	inhibitory activity of shrimp waste extracts on fungal and oomycete plant pathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2df40ca8f1bb40489b6b8242648ba61a
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_version_	1718410701262815232

Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

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