Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.

Cotton fiber is an ideal model to study cell elongation and cell wall construction in plants. During fiber development, some genes and proteins have been reported to be specifically or preferentially expressed. Mapping of them will reveal the genomic distribution of these genes, and will facilitate...

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Autores principales:	Ximei Li, Daojun Yuan, Jinfa Zhang, Zhongxu Lin, Xianlong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2df7b9bf0b5945bc835aa7fed65b3524
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spelling	oai:doaj.org-article:2df7b9bf0b5945bc835aa7fed65b35242021-11-18T07:59:51ZGenetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.1932-620310.1371/journal.pone.0054444https://doaj.org/article/2df7b9bf0b5945bc835aa7fed65b35242013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23372723/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cotton fiber is an ideal model to study cell elongation and cell wall construction in plants. During fiber development, some genes and proteins have been reported to be specifically or preferentially expressed. Mapping of them will reveal the genomic distribution of these genes, and will facilitate selection in cotton breeding. Based on previous reports, we designed 331 gene primers and 164 protein primers, and used single-strand conformation polymorphism (SSCP) to map and integrate them into our interspecific BC(1) linkage map. This resulted in the mapping of 57 loci representing 51 genes or proteins on 22 chromosomes. For those three markers which were tightly linked with quantitative trait loci (QTLs), the QTL functions obtained in this study and gene functions reported in previous reports were consistent. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of 52 polymorphic functional primers showed that 21 gene primers and 17 protein primers had differential expression between Emian22 (Gossypium hirsutum) and 3-79 (G. barbadense). Both RT-PCR and quantitative real-time PCR (qRT-PCR) analyses of the three markers tightly linked with QTLs were consistent with QTL analysis and field experiments. Gene Ontology (GO) categorization revealed that almost all 51 mapped genes belonged to multiple categories that contribute to fiber development, indicating that fiber development is a complex process regulated by various genes. These 51 genes were all specifically or preferentially expressed during fiber cell elongation and secondary wall biosynthesis. Therefore, these functional gene-related markers would be beneficial for the genetic improvement of cotton fiber length and strength.Ximei LiDaojun YuanJinfa ZhangZhongxu LinXianlong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54444 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ximei Li Daojun Yuan Jinfa Zhang Zhongxu Lin Xianlong Zhang Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
description	Cotton fiber is an ideal model to study cell elongation and cell wall construction in plants. During fiber development, some genes and proteins have been reported to be specifically or preferentially expressed. Mapping of them will reveal the genomic distribution of these genes, and will facilitate selection in cotton breeding. Based on previous reports, we designed 331 gene primers and 164 protein primers, and used single-strand conformation polymorphism (SSCP) to map and integrate them into our interspecific BC(1) linkage map. This resulted in the mapping of 57 loci representing 51 genes or proteins on 22 chromosomes. For those three markers which were tightly linked with quantitative trait loci (QTLs), the QTL functions obtained in this study and gene functions reported in previous reports were consistent. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of 52 polymorphic functional primers showed that 21 gene primers and 17 protein primers had differential expression between Emian22 (Gossypium hirsutum) and 3-79 (G. barbadense). Both RT-PCR and quantitative real-time PCR (qRT-PCR) analyses of the three markers tightly linked with QTLs were consistent with QTL analysis and field experiments. Gene Ontology (GO) categorization revealed that almost all 51 mapped genes belonged to multiple categories that contribute to fiber development, indicating that fiber development is a complex process regulated by various genes. These 51 genes were all specifically or preferentially expressed during fiber cell elongation and secondary wall biosynthesis. Therefore, these functional gene-related markers would be beneficial for the genetic improvement of cotton fiber length and strength.
format	article
author	Ximei Li Daojun Yuan Jinfa Zhang Zhongxu Lin Xianlong Zhang
author_facet	Ximei Li Daojun Yuan Jinfa Zhang Zhongxu Lin Xianlong Zhang
author_sort	Ximei Li
title	Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
title_short	Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
title_full	Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
title_fullStr	Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
title_full_unstemmed	Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
title_sort	genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2df7b9bf0b5945bc835aa7fed65b3524
work_keys_str_mv	AT ximeili geneticmappingandcharacteristicsofgenesspecificallyorpreferentiallyexpressedduringfiberdevelopmentincotton AT daojunyuan geneticmappingandcharacteristicsofgenesspecificallyorpreferentiallyexpressedduringfiberdevelopmentincotton AT jinfazhang geneticmappingandcharacteristicsofgenesspecificallyorpreferentiallyexpressedduringfiberdevelopmentincotton AT zhongxulin geneticmappingandcharacteristicsofgenesspecificallyorpreferentiallyexpressedduringfiberdevelopmentincotton AT xianlongzhang geneticmappingandcharacteristicsofgenesspecificallyorpreferentiallyexpressedduringfiberdevelopmentincotton
_version_	1718422654976786432

Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.

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