Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides

Abstract HLA class II (HLA-II) genes’ polymorphism influences the immune response to Chlamydia trachomatis (Ct), it is considered a sexually transmitted infection. However, associations between HLA-II alleles and Ct-infection have been little explored in humans; this study was thus aimed at determin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Leidy Pedraza, Milena Camargo, Darwin A. Moreno-Pérez, Ricardo Sánchez, Luisa Del Río-Ospina, Indira M. Báez-Murcia, Manuel E. Patarroyo, Manuel A. Patarroyo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2dfac16284344430a5195b7de5dabe33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2dfac16284344430a5195b7de5dabe33
record_format	dspace
spelling	oai:doaj.org-article:2dfac16284344430a5195b7de5dabe332021-12-02T17:40:06ZIdentifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides10.1038/s41598-021-92294-w2045-2322https://doaj.org/article/2dfac16284344430a5195b7de5dabe332021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92294-whttps://doaj.org/toc/2045-2322Abstract HLA class II (HLA-II) genes’ polymorphism influences the immune response to Chlamydia trachomatis (Ct), it is considered a sexually transmitted infection. However, associations between HLA-II alleles and Ct-infection have been little explored in humans; this study was thus aimed at determining HLA-DRB1-DQB1 alleles/haplotypes’ effect on Ct-infection outcome in a cohort of Colombian women. Cervical sample DNA was used as template for detecting Ct by PCR and typing HLA-DRB1-DQB1 alleles/haplotypes by Illumina MiSeq sequencing. Survival models were adjusted for identifying the alleles/haplotypes’ effect on Ct-outcome; bioinformatics tools were used for predicting secreted bacterial protein T- and B-cell epitopes. Sixteen HLA-DRB1 alleles having a significant effect on Ct-outcome were identified in the 262 women analysed. DRB108:02:01G and DRB112:01:01G were related to infection-promoting events. Only the DQB1*05:03:01G allele related to clearance/persistence events was found for HLA-DQB1. HLA-DRB1 allele homozygous women were associated with events having a lower probability of clearance and/or early occurrence of persistence. Twenty-seven peptides predicted in silico were associated with protective immunity against Ct; outer membrane and polymorphic membrane protein-derived peptides had regions having dual potential for being T- or B-cell epitopes. This article describes HLA-DRB1-DQB1 alleles/haplotypes related to Ct-infection resolution and the peptides predicted in silico which might probably be involved in host immune response. The data provides base information for developing future studies leading to the development of effective prevention measures against Ct-infection.Leidy PedrazaMilena CamargoDarwin A. Moreno-PérezRicardo SánchezLuisa Del Río-OspinaIndira M. Báez-MurciaManuel E. PatarroyoManuel A. PatarroyoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leidy Pedraza Milena Camargo Darwin A. Moreno-Pérez Ricardo Sánchez Luisa Del Río-Ospina Indira M. Báez-Murcia Manuel E. Patarroyo Manuel A. Patarroyo Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides
description	Abstract HLA class II (HLA-II) genes’ polymorphism influences the immune response to Chlamydia trachomatis (Ct), it is considered a sexually transmitted infection. However, associations between HLA-II alleles and Ct-infection have been little explored in humans; this study was thus aimed at determining HLA-DRB1-DQB1 alleles/haplotypes’ effect on Ct-infection outcome in a cohort of Colombian women. Cervical sample DNA was used as template for detecting Ct by PCR and typing HLA-DRB1-DQB1 alleles/haplotypes by Illumina MiSeq sequencing. Survival models were adjusted for identifying the alleles/haplotypes’ effect on Ct-outcome; bioinformatics tools were used for predicting secreted bacterial protein T- and B-cell epitopes. Sixteen HLA-DRB1 alleles having a significant effect on Ct-outcome were identified in the 262 women analysed. DRB108:02:01G and DRB112:01:01G were related to infection-promoting events. Only the DQB1*05:03:01G allele related to clearance/persistence events was found for HLA-DQB1. HLA-DRB1 allele homozygous women were associated with events having a lower probability of clearance and/or early occurrence of persistence. Twenty-seven peptides predicted in silico were associated with protective immunity against Ct; outer membrane and polymorphic membrane protein-derived peptides had regions having dual potential for being T- or B-cell epitopes. This article describes HLA-DRB1-DQB1 alleles/haplotypes related to Ct-infection resolution and the peptides predicted in silico which might probably be involved in host immune response. The data provides base information for developing future studies leading to the development of effective prevention measures against Ct-infection.
format	article
author	Leidy Pedraza Milena Camargo Darwin A. Moreno-Pérez Ricardo Sánchez Luisa Del Río-Ospina Indira M. Báez-Murcia Manuel E. Patarroyo Manuel A. Patarroyo
author_facet	Leidy Pedraza Milena Camargo Darwin A. Moreno-Pérez Ricardo Sánchez Luisa Del Río-Ospina Indira M. Báez-Murcia Manuel E. Patarroyo Manuel A. Patarroyo
author_sort	Leidy Pedraza
title	Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides
title_short	Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides
title_full	Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides
title_fullStr	Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides
title_full_unstemmed	Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides
title_sort	identifying hla drb1-dqb1 alleles associated with chlamydia trachomatis infection and in silico prediction of potentially-related peptides
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2dfac16284344430a5195b7de5dabe33
work_keys_str_mv	AT leidypedraza identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT milenacamargo identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT darwinamorenoperez identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT ricardosanchez identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT luisadelrioospina identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT indirambaezmurcia identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT manuelepatarroyo identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides AT manuelapatarroyo identifyinghladrb1dqb1allelesassociatedwithchlamydiatrachomatisinfectionandinsilicopredictionofpotentiallyrelatedpeptides
_version_	1718379753377890304

Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides

Ejemplares similares