Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

Donghyun Lee,1 Dong Nyoung Heo,2 Ha Ram Nah,3 Sang Jin Lee,1 Wan-Kyu Ko,4 Jae Seo Lee,3 Ho-Jin Moon,1 Jae Beum Bang,5 Yu-Shik Hwang,6 Rui L Reis,1,7 Il Keun Kwon1 1Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 2Department of Engineering S...

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Autores principales:	Lee D, Heo DN, Nah HR, Lee SJ, Ko WK, Lee JS, Moon HJ, Bang JB, Hwang YS, Reis RL, Kwon IK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Nanomaterial Gelatin Enzymatic cross-linking Osteogenesis N-acetyl cysteine. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2e0495320d1d41788bb8cdf56ca7f7dd
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spelling	oai:doaj.org-article:2e0495320d1d41788bb8cdf56ca7f7dd2021-12-02T03:09:21ZInjectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration1178-2013https://doaj.org/article/2e0495320d1d41788bb8cdf56ca7f7dd2018-11-01T00:00:00Zhttps://www.dovepress.com/injectable-hydrogel-composite-containing-modified-gold-nanoparticles-i-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Donghyun Lee,1 Dong Nyoung Heo,2 Ha Ram Nah,3 Sang Jin Lee,1 Wan-Kyu Ko,4 Jae Seo Lee,3 Ho-Jin Moon,1 Jae Beum Bang,5 Yu-Shik Hwang,6 Rui L Reis,1,7 Il Keun Kwon1 1Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 2Department of Engineering Science and Mechanics, Pennsylvania State University, Pennsylvania 16802, USA; 3Department of Detistry, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; 4Department of Neurosurgery, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 13496, Republic of Korea; 5Department of Dental Education, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 6Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 7The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Barco, Guimarães, Portugal Background: For effective bone regeneration, it is necessary to implant a biocompatible scaffold that is capable of inducing cell growth and continuous osteogenic stimulation at the defected site. Here, we suggest an injectable hydrogel system using enzymatic cross-linkable gelatin (Gel) and functionalized gold nanoparticles (GNPs). Methods: In this work, tyramine (Ty) was synthesized on the gelatin backbone (Gel-Ty) to enable a phenol crosslinking reaction with horseradish peroxidase (HRP). N-acetyl cysteine (NAC) was attached to the GNPs surface (G-NAC) for promoting osteo-differentiation. Results: The Gel-Ty hydrogels containing G-NAC (Gel-Ty/G-NAC) had suitable mechanical strength and biocompatibility to embed and support the growth of human adipose derived stem cells (hASCs) during a proliferation test for three days. In addition, G-NAC promoted osteo-differentiation both when it was included in Gel-Ty and when it was used directly in hASCs. The osteogenic effects were demonstrated by the alkaline phosphatase (ALP) activity test. Conclusion: These findings indicate that the phenol crosslinking reaction is suitable for injectable hydrogels for tissue regeneration and G-NAC stimulate bone regeneration. Based on our results, we suggest that Gel-Ty/G-NAC hydrogels can serve both as a biodegradable graft material for bone defect treatment and as a good template for tissue engineering applications such as drug delivery, cell delivery, and various tissue regeneration uses. Keywords: nanomaterial, gelatin, enzymatic cross-linking, osteogenesis, N-acetyl cysteineLee DHeo DNNah HRLee SJKo WKLee JSMoon HJBang JBHwang YSReis RLKwon IKDove Medical PressarticleNanomaterialGelatinEnzymatic cross-linkingOsteogenesisN-acetyl cysteine.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 7019-7031 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanomaterial Gelatin Enzymatic cross-linking Osteogenesis N-acetyl cysteine. Medicine (General) R5-920
spellingShingle	Nanomaterial Gelatin Enzymatic cross-linking Osteogenesis N-acetyl cysteine. Medicine (General) R5-920 Lee D Heo DN Nah HR Lee SJ Ko WK Lee JS Moon HJ Bang JB Hwang YS Reis RL Kwon IK Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
description	Donghyun Lee,1 Dong Nyoung Heo,2 Ha Ram Nah,3 Sang Jin Lee,1 Wan-Kyu Ko,4 Jae Seo Lee,3 Ho-Jin Moon,1 Jae Beum Bang,5 Yu-Shik Hwang,6 Rui L Reis,1,7 Il Keun Kwon1 1Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 2Department of Engineering Science and Mechanics, Pennsylvania State University, Pennsylvania 16802, USA; 3Department of Detistry, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; 4Department of Neurosurgery, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 13496, Republic of Korea; 5Department of Dental Education, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 6Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea; 7The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Barco, Guimarães, Portugal Background: For effective bone regeneration, it is necessary to implant a biocompatible scaffold that is capable of inducing cell growth and continuous osteogenic stimulation at the defected site. Here, we suggest an injectable hydrogel system using enzymatic cross-linkable gelatin (Gel) and functionalized gold nanoparticles (GNPs). Methods: In this work, tyramine (Ty) was synthesized on the gelatin backbone (Gel-Ty) to enable a phenol crosslinking reaction with horseradish peroxidase (HRP). N-acetyl cysteine (NAC) was attached to the GNPs surface (G-NAC) for promoting osteo-differentiation. Results: The Gel-Ty hydrogels containing G-NAC (Gel-Ty/G-NAC) had suitable mechanical strength and biocompatibility to embed and support the growth of human adipose derived stem cells (hASCs) during a proliferation test for three days. In addition, G-NAC promoted osteo-differentiation both when it was included in Gel-Ty and when it was used directly in hASCs. The osteogenic effects were demonstrated by the alkaline phosphatase (ALP) activity test. Conclusion: These findings indicate that the phenol crosslinking reaction is suitable for injectable hydrogels for tissue regeneration and G-NAC stimulate bone regeneration. Based on our results, we suggest that Gel-Ty/G-NAC hydrogels can serve both as a biodegradable graft material for bone defect treatment and as a good template for tissue engineering applications such as drug delivery, cell delivery, and various tissue regeneration uses. Keywords: nanomaterial, gelatin, enzymatic cross-linking, osteogenesis, N-acetyl cysteine
format	article
author	Lee D Heo DN Nah HR Lee SJ Ko WK Lee JS Moon HJ Bang JB Hwang YS Reis RL Kwon IK
author_facet	Lee D Heo DN Nah HR Lee SJ Ko WK Lee JS Moon HJ Bang JB Hwang YS Reis RL Kwon IK
author_sort	Lee D
title	Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
title_short	Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
title_full	Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
title_fullStr	Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
title_full_unstemmed	Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
title_sort	injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/2e0495320d1d41788bb8cdf56ca7f7dd
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_version_	1718401908515799040

Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

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