Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line.
Galectin-9 (Gal-9), a lectin having a β-galactoside-binding domain, can induce apoptosis of Th1 cells by binding to TIM-3. In addition, Gal-9 inhibits IgE/Ag-mediated degranulation of mast cell/basophilic cell lines by binding to IgE, thus blocking IgE/Ag complex formation. However, the role of Gal-...
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oai:doaj.org-article:2e08c6e91f53490aa0974e16fd19a4362021-11-18T08:37:11ZGalectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line.1932-620310.1371/journal.pone.0086106https://doaj.org/article/2e08c6e91f53490aa0974e16fd19a4362014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465902/?tool=EBIhttps://doaj.org/toc/1932-6203Galectin-9 (Gal-9), a lectin having a β-galactoside-binding domain, can induce apoptosis of Th1 cells by binding to TIM-3. In addition, Gal-9 inhibits IgE/Ag-mediated degranulation of mast cell/basophilic cell lines by binding to IgE, thus blocking IgE/Ag complex formation. However, the role of Gal-9 in mast cell function in the absence of IgE is not fully understood. Here, we found that recombinant Gal-9 directly induced phosphorylation of Erk1/2 but not p38 MAPK in a human mast cell line, HMC-1, which does not express FcεRI. Gal-9 induced apoptosis and inhibited PMA/ionomycin-mediated degranulation of HMC-1 cells. On the other hand, Gal-9 induced cytokine and/or chemokine production by HMC-1 cells, dependent on activation of ERK1/2 but not p38 MAPK. In addition, the lectin activity of Gal-9 was required for Gal-9-mediated cytokine secretion by HMC-1 cells. These observations suggest that Gal-9 has dual properties as both a regulator and an activator of mast cells.Reiji KojimaTatsukuni OhnoMotoyasu IikuraToshiro NikiMitsuomi HirashimaKeichi IwayaHitoshi TsudaShigeaki NonoyamaAkio MatsudaHirohisa SaitoKenji MatsumotoSusumu NakaePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86106 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Reiji Kojima Tatsukuni Ohno Motoyasu Iikura Toshiro Niki Mitsuomi Hirashima Keichi Iwaya Hitoshi Tsuda Shigeaki Nonoyama Akio Matsuda Hirohisa Saito Kenji Matsumoto Susumu Nakae Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| description |
Galectin-9 (Gal-9), a lectin having a β-galactoside-binding domain, can induce apoptosis of Th1 cells by binding to TIM-3. In addition, Gal-9 inhibits IgE/Ag-mediated degranulation of mast cell/basophilic cell lines by binding to IgE, thus blocking IgE/Ag complex formation. However, the role of Gal-9 in mast cell function in the absence of IgE is not fully understood. Here, we found that recombinant Gal-9 directly induced phosphorylation of Erk1/2 but not p38 MAPK in a human mast cell line, HMC-1, which does not express FcεRI. Gal-9 induced apoptosis and inhibited PMA/ionomycin-mediated degranulation of HMC-1 cells. On the other hand, Gal-9 induced cytokine and/or chemokine production by HMC-1 cells, dependent on activation of ERK1/2 but not p38 MAPK. In addition, the lectin activity of Gal-9 was required for Gal-9-mediated cytokine secretion by HMC-1 cells. These observations suggest that Gal-9 has dual properties as both a regulator and an activator of mast cells. |
| format |
article |
| author |
Reiji Kojima Tatsukuni Ohno Motoyasu Iikura Toshiro Niki Mitsuomi Hirashima Keichi Iwaya Hitoshi Tsuda Shigeaki Nonoyama Akio Matsuda Hirohisa Saito Kenji Matsumoto Susumu Nakae |
| author_facet |
Reiji Kojima Tatsukuni Ohno Motoyasu Iikura Toshiro Niki Mitsuomi Hirashima Keichi Iwaya Hitoshi Tsuda Shigeaki Nonoyama Akio Matsuda Hirohisa Saito Kenji Matsumoto Susumu Nakae |
| author_sort |
Reiji Kojima |
| title |
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| title_short |
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| title_full |
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| title_fullStr |
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| title_full_unstemmed |
Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| title_sort |
galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/2e08c6e91f53490aa0974e16fd19a436 |
| work_keys_str_mv |
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