Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia

Abstract Oxygen delivery to the retinal pigment epithelium and the outer retina is essential for metabolism, function, and survival of photoreceptors. Chronically reduced oxygen supply leads to retinal pathologies in patients and causes age-dependent retinal degeneration in mice. Hypoxia can result...

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Autores principales:	L. J. A. Ebner, M. Samardzija, F. Storti, V. Todorova, D. Karademir, J. Behr, F. Simpson, M. Thiersch, C. Grimm
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e0a9ed0f2764e6189d2cbe340dd2dc0
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spelling	oai:doaj.org-article:2e0a9ed0f2764e6189d2cbe340dd2dc02021-12-02T18:51:52ZTranscriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia10.1038/s41598-021-96150-92045-2322https://doaj.org/article/2e0a9ed0f2764e6189d2cbe340dd2dc02021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96150-9https://doaj.org/toc/2045-2322Abstract Oxygen delivery to the retinal pigment epithelium and the outer retina is essential for metabolism, function, and survival of photoreceptors. Chronically reduced oxygen supply leads to retinal pathologies in patients and causes age-dependent retinal degeneration in mice. Hypoxia can result from decreased levels of inspired oxygen (normobaric hypoxia) or reduced barometric pressure (hypobaric hypoxia). Since the response of retinal cells to chronic normobaric or hypobaric hypoxia is mostly unknown, we examined the effect of six hypoxic conditions on the retinal transcriptome and photoreceptor morphology. Mice were exposed to short- and long-term normobaric hypoxia at 400 m or hypobaric hypoxia at 3450 m above sea level. Longitudinal studies over 11 weeks in normobaric hypoxia revealed four classes of genes that adapted differentially to the hypoxic condition. Seventeen genes were specifically regulated in hypobaric hypoxia and may affect the structural integrity of the retina, resulting in the shortening of photoreceptor segment length detected in various hypoxic groups. This study shows that retinal cells have the capacity to adapt to long-term hypoxia and that consequences of hypobaric hypoxia differ from those of normobaric hypoxia. Our datasets can be used as references to validate and compare retinal disease models associated with hypoxia.L. J. A. EbnerM. SamardzijaF. StortiV. TodorovaD. KarademirJ. BehrF. SimpsonM. ThierschC. GrimmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. J. A. Ebner M. Samardzija F. Storti V. Todorova D. Karademir J. Behr F. Simpson M. Thiersch C. Grimm Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
description	Abstract Oxygen delivery to the retinal pigment epithelium and the outer retina is essential for metabolism, function, and survival of photoreceptors. Chronically reduced oxygen supply leads to retinal pathologies in patients and causes age-dependent retinal degeneration in mice. Hypoxia can result from decreased levels of inspired oxygen (normobaric hypoxia) or reduced barometric pressure (hypobaric hypoxia). Since the response of retinal cells to chronic normobaric or hypobaric hypoxia is mostly unknown, we examined the effect of six hypoxic conditions on the retinal transcriptome and photoreceptor morphology. Mice were exposed to short- and long-term normobaric hypoxia at 400 m or hypobaric hypoxia at 3450 m above sea level. Longitudinal studies over 11 weeks in normobaric hypoxia revealed four classes of genes that adapted differentially to the hypoxic condition. Seventeen genes were specifically regulated in hypobaric hypoxia and may affect the structural integrity of the retina, resulting in the shortening of photoreceptor segment length detected in various hypoxic groups. This study shows that retinal cells have the capacity to adapt to long-term hypoxia and that consequences of hypobaric hypoxia differ from those of normobaric hypoxia. Our datasets can be used as references to validate and compare retinal disease models associated with hypoxia.
format	article
author	L. J. A. Ebner M. Samardzija F. Storti V. Todorova D. Karademir J. Behr F. Simpson M. Thiersch C. Grimm
author_facet	L. J. A. Ebner M. Samardzija F. Storti V. Todorova D. Karademir J. Behr F. Simpson M. Thiersch C. Grimm
author_sort	L. J. A. Ebner
title	Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
title_short	Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
title_full	Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
title_fullStr	Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
title_full_unstemmed	Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
title_sort	transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2e0a9ed0f2764e6189d2cbe340dd2dc0
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Transcriptomic analysis of the mouse retina after acute and chronic normobaric and hypobaric hypoxia

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