The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens

Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagno...

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Autores principales:	Aleksandr V. Ivanov, Irina V. Safenkova, Anatoly V. Zherdev, Boris B. Dzantiev
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	out-of-lab diagnostics molecular diagnostics plant diseases isothermal amplification DNA amplicon detection recombinase polymerase amplification Botany QK1-989
Acceso en línea:	https://doaj.org/article/2e193732d1b34f5b9bedb9bfc5483648
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spelling	oai:doaj.org-article:2e193732d1b34f5b9bedb9bfc54836482021-11-25T18:46:29ZThe Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens10.3390/plants101124242223-7747https://doaj.org/article/2e193732d1b34f5b9bedb9bfc54836482021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2424https://doaj.org/toc/2223-7747Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossible. With the advent of isothermal amplification methods, which provide amplification of nucleic acids at a certain temperature and do not require thermocyclic equipment, going beyond the laboratory has become a reality for molecular diagnostics. The amplification stage ceases to be limited by time and instruments. Challenges to solve involve finding suitable approaches for rapid and user-friendly plant preparation and detection of amplicons after amplification. Here, we summarize approaches for in-field diagnostics of phytopathogens based on different types of isothermal amplification and discuss their advantages and disadvantages. In this review, we consider a combination of isothermal amplification methods with extraction and detection methods compatible with in-field phytodiagnostics. Molecular diagnostics in out-of-lab conditions are of particular importance for protecting against viral, bacterial, and fungal phytopathogens in order to quickly prevent and control the spread of disease. We believe that the development of rapid, sensitive, and equipment-free nucleic acid detection methods is the future of phytodiagnostics, and its benefits are already visible.Aleksandr V. IvanovIrina V. SafenkovaAnatoly V. ZherdevBoris B. DzantievMDPI AGarticleout-of-lab diagnosticsmolecular diagnosticsplant diseasesisothermal amplificationDNA amplicon detectionrecombinase polymerase amplificationBotanyQK1-989ENPlants, Vol 10, Iss 2424, p 2424 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	out-of-lab diagnostics molecular diagnostics plant diseases isothermal amplification DNA amplicon detection recombinase polymerase amplification Botany QK1-989
spellingShingle	out-of-lab diagnostics molecular diagnostics plant diseases isothermal amplification DNA amplicon detection recombinase polymerase amplification Botany QK1-989 Aleksandr V. Ivanov Irina V. Safenkova Anatoly V. Zherdev Boris B. Dzantiev The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
description	Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossible. With the advent of isothermal amplification methods, which provide amplification of nucleic acids at a certain temperature and do not require thermocyclic equipment, going beyond the laboratory has become a reality for molecular diagnostics. The amplification stage ceases to be limited by time and instruments. Challenges to solve involve finding suitable approaches for rapid and user-friendly plant preparation and detection of amplicons after amplification. Here, we summarize approaches for in-field diagnostics of phytopathogens based on different types of isothermal amplification and discuss their advantages and disadvantages. In this review, we consider a combination of isothermal amplification methods with extraction and detection methods compatible with in-field phytodiagnostics. Molecular diagnostics in out-of-lab conditions are of particular importance for protecting against viral, bacterial, and fungal phytopathogens in order to quickly prevent and control the spread of disease. We believe that the development of rapid, sensitive, and equipment-free nucleic acid detection methods is the future of phytodiagnostics, and its benefits are already visible.
format	article
author	Aleksandr V. Ivanov Irina V. Safenkova Anatoly V. Zherdev Boris B. Dzantiev
author_facet	Aleksandr V. Ivanov Irina V. Safenkova Anatoly V. Zherdev Boris B. Dzantiev
author_sort	Aleksandr V. Ivanov
title	The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
title_short	The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
title_full	The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
title_fullStr	The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
title_full_unstemmed	The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
title_sort	potential use of isothermal amplification assays for in-field diagnostics of plant pathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2e193732d1b34f5b9bedb9bfc5483648
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The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens

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