Establishment and genomic characterization of the new chordoma cell line Chor-IN-1

Abstract Chordomas are rare, slowly growing tumors with high medical need, arising in the axial skeleton from notochord remnants. The transcription factor “brachyury” represents a distinctive molecular marker and a key oncogenic driver of chordomas. Tyrosine kinase receptors are also expressed, but...

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Autores principales:	Roberta Bosotti, Paola Magnaghi, Sebastiano Di Bella, Liviana Cozzi, Carlo Cusi, Fabio Bozzi, Nicola Beltrami, Giovanni Carapezza, Dario Ballinari, Nadia Amboldi, Rosita Lupi, Alessio Somaschini, Laura Raddrizzani, Barbara Salom, Arturo Galvani, Silvia Stacchiotti, Elena Tamborini, Antonella Isacchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e2d8fd735034e6896516334379601fa
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spelling	oai:doaj.org-article:2e2d8fd735034e6896516334379601fa2021-12-02T12:30:13ZEstablishment and genomic characterization of the new chordoma cell line Chor-IN-110.1038/s41598-017-10044-32045-2322https://doaj.org/article/2e2d8fd735034e6896516334379601fa2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10044-3https://doaj.org/toc/2045-2322Abstract Chordomas are rare, slowly growing tumors with high medical need, arising in the axial skeleton from notochord remnants. The transcription factor “brachyury” represents a distinctive molecular marker and a key oncogenic driver of chordomas. Tyrosine kinase receptors are also expressed, but so far kinase inhibitors have not shown clear clinical efficacy in chordoma patients. The need for effective therapies is extremely high, but the paucity of established chordoma cell lines has limited preclinical research. Here we describe the isolation of the new Chor-IN-1 cell line from a recurrent sacral chordoma and its characterization as compared to other chordoma cell lines. Chor-IN-1 displays genomic identity to the tumor of origin and has morphological features, growth characteristics and chromosomal abnormalities typical of chordoma, with expression of brachyury and other relevant biomarkers. Chor-IN-1 gene variants, copy number alterations and kinome gene expression were analyzed in comparison to other four chordoma cell lines, generating large scale DNA and mRNA genomic data that can be exploited for the identification of novel pharmacological targets and candidate predictive biomarkers of drug sensitivity in chordoma. The establishment of this new, well characterized chordoma cell line provides a useful tool for the identification of drugs active in chordoma.Roberta BosottiPaola MagnaghiSebastiano Di BellaLiviana CozziCarlo CusiFabio BozziNicola BeltramiGiovanni CarapezzaDario BallinariNadia AmboldiRosita LupiAlessio SomaschiniLaura RaddrizzaniBarbara SalomArturo GalvaniSilvia StacchiottiElena TamboriniAntonella IsacchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roberta Bosotti Paola Magnaghi Sebastiano Di Bella Liviana Cozzi Carlo Cusi Fabio Bozzi Nicola Beltrami Giovanni Carapezza Dario Ballinari Nadia Amboldi Rosita Lupi Alessio Somaschini Laura Raddrizzani Barbara Salom Arturo Galvani Silvia Stacchiotti Elena Tamborini Antonella Isacchi Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
description	Abstract Chordomas are rare, slowly growing tumors with high medical need, arising in the axial skeleton from notochord remnants. The transcription factor “brachyury” represents a distinctive molecular marker and a key oncogenic driver of chordomas. Tyrosine kinase receptors are also expressed, but so far kinase inhibitors have not shown clear clinical efficacy in chordoma patients. The need for effective therapies is extremely high, but the paucity of established chordoma cell lines has limited preclinical research. Here we describe the isolation of the new Chor-IN-1 cell line from a recurrent sacral chordoma and its characterization as compared to other chordoma cell lines. Chor-IN-1 displays genomic identity to the tumor of origin and has morphological features, growth characteristics and chromosomal abnormalities typical of chordoma, with expression of brachyury and other relevant biomarkers. Chor-IN-1 gene variants, copy number alterations and kinome gene expression were analyzed in comparison to other four chordoma cell lines, generating large scale DNA and mRNA genomic data that can be exploited for the identification of novel pharmacological targets and candidate predictive biomarkers of drug sensitivity in chordoma. The establishment of this new, well characterized chordoma cell line provides a useful tool for the identification of drugs active in chordoma.
format	article
author	Roberta Bosotti Paola Magnaghi Sebastiano Di Bella Liviana Cozzi Carlo Cusi Fabio Bozzi Nicola Beltrami Giovanni Carapezza Dario Ballinari Nadia Amboldi Rosita Lupi Alessio Somaschini Laura Raddrizzani Barbara Salom Arturo Galvani Silvia Stacchiotti Elena Tamborini Antonella Isacchi
author_facet	Roberta Bosotti Paola Magnaghi Sebastiano Di Bella Liviana Cozzi Carlo Cusi Fabio Bozzi Nicola Beltrami Giovanni Carapezza Dario Ballinari Nadia Amboldi Rosita Lupi Alessio Somaschini Laura Raddrizzani Barbara Salom Arturo Galvani Silvia Stacchiotti Elena Tamborini Antonella Isacchi
author_sort	Roberta Bosotti
title	Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
title_short	Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
title_full	Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
title_fullStr	Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
title_full_unstemmed	Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
title_sort	establishment and genomic characterization of the new chordoma cell line chor-in-1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2e2d8fd735034e6896516334379601fa
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Establishment and genomic characterization of the new chordoma cell line Chor-IN-1

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