Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.

<h4>Background</h4>Nucleosomal histones are intracellular proteins that are highly conserved among Leishmania species. After parasite destruction or spontaneous lysis, exposure to these proteins elicits a strong host immune response. In the present study, we analyzed the protective capab...

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Autores principales:	Marcia W Carneiro, Diego M Santos, Kiyoshi F Fukutani, Jorge Clarencio, Jose Carlos Miranda, Claudia Brodskyn, Aldina Barral, Manoel Barral-Netto, Manuel Soto, Camila I de Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e3759e8014e410c860ab16b24f3192e
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spelling	oai:doaj.org-article:2e3759e8014e410c860ab16b24f3192e2021-11-18T08:04:12ZVaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.1932-620310.1371/journal.pone.0052296https://doaj.org/article/2e3759e8014e410c860ab16b24f3192e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23284976/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Nucleosomal histones are intracellular proteins that are highly conserved among Leishmania species. After parasite destruction or spontaneous lysis, exposure to these proteins elicits a strong host immune response. In the present study, we analyzed the protective capability of Leishmania infantum chagasi nucleosomal histones against L. braziliensis infection using different immunization strategies.<h4>Methodology/principal findings</h4>BALB/c mice were immunized with either a plasmid DNA cocktail (DNA) containing four Leishmania nucleosomal histones or with the DNA cocktail followed by the corresponding recombinant proteins plus CpG (DNA/Protein). Mice were later challenged with L. braziliensis, in the presence of sand fly saliva. Lesion development, parasite load and the cellular immune response were analyzed five weeks after challenge. Immunization with either DNA alone or with DNA/Protein was able to inhibit lesion development. This finding was highlighted by the absence of infected macrophages in tissue sections. Further, parasite load at the infection site and in the draining lymph nodes was also significantly lower in vaccinated animals. This outcome was associated with increased expression of IFN-γ and down regulation of IL-4 at the infection site.<h4>Conclusion</h4>The data presented here demonstrate the potential use of L. infantum chagasi nucleosomal histones as targets for the development of vaccines against infection with L. braziliensis, as shown by the significant inhibition of disease development following a live challenge.Marcia W CarneiroDiego M SantosKiyoshi F FukutaniJorge ClarencioJose Carlos MirandaClaudia BrodskynAldina BarralManoel Barral-NettoManuel SotoCamila I de OliveiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52296 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcia W Carneiro Diego M Santos Kiyoshi F Fukutani Jorge Clarencio Jose Carlos Miranda Claudia Brodskyn Aldina Barral Manoel Barral-Netto Manuel Soto Camila I de Oliveira Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.
description	<h4>Background</h4>Nucleosomal histones are intracellular proteins that are highly conserved among Leishmania species. After parasite destruction or spontaneous lysis, exposure to these proteins elicits a strong host immune response. In the present study, we analyzed the protective capability of Leishmania infantum chagasi nucleosomal histones against L. braziliensis infection using different immunization strategies.<h4>Methodology/principal findings</h4>BALB/c mice were immunized with either a plasmid DNA cocktail (DNA) containing four Leishmania nucleosomal histones or with the DNA cocktail followed by the corresponding recombinant proteins plus CpG (DNA/Protein). Mice were later challenged with L. braziliensis, in the presence of sand fly saliva. Lesion development, parasite load and the cellular immune response were analyzed five weeks after challenge. Immunization with either DNA alone or with DNA/Protein was able to inhibit lesion development. This finding was highlighted by the absence of infected macrophages in tissue sections. Further, parasite load at the infection site and in the draining lymph nodes was also significantly lower in vaccinated animals. This outcome was associated with increased expression of IFN-γ and down regulation of IL-4 at the infection site.<h4>Conclusion</h4>The data presented here demonstrate the potential use of L. infantum chagasi nucleosomal histones as targets for the development of vaccines against infection with L. braziliensis, as shown by the significant inhibition of disease development following a live challenge.
format	article
author	Marcia W Carneiro Diego M Santos Kiyoshi F Fukutani Jorge Clarencio Jose Carlos Miranda Claudia Brodskyn Aldina Barral Manoel Barral-Netto Manuel Soto Camila I de Oliveira
author_facet	Marcia W Carneiro Diego M Santos Kiyoshi F Fukutani Jorge Clarencio Jose Carlos Miranda Claudia Brodskyn Aldina Barral Manoel Barral-Netto Manuel Soto Camila I de Oliveira
author_sort	Marcia W Carneiro
title	Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.
title_short	Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.
title_full	Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.
title_fullStr	Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.
title_full_unstemmed	Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.
title_sort	vaccination with l. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by leishmania braziliensis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2e3759e8014e410c860ab16b24f3192e
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Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis.

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