Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae

Abstract Burkholderia glumae is a causal agent of bacterial grain and seedling rot in rice, and is a threat to stable global food supply. The virulence of B. glumae was suppressed when it was inoculated on budding seed rather than on non-budding seed. To clarify the phenomena, pathogen titer inside...

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Autor principal:	Chiharu Akimoto-Tomiyama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e39e6daa2e14fe1a3139bc6ea5d7e41
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spelling	oai:doaj.org-article:2e39e6daa2e14fe1a3139bc6ea5d7e412021-12-02T14:21:53ZMultiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae10.1038/s41598-021-83794-w2045-2322https://doaj.org/article/2e39e6daa2e14fe1a3139bc6ea5d7e412021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83794-whttps://doaj.org/toc/2045-2322Abstract Burkholderia glumae is a causal agent of bacterial grain and seedling rot in rice, and is a threat to stable global food supply. The virulence of B. glumae was suppressed when it was inoculated on budding seed rather than on non-budding seed. To clarify the phenomena, pathogen titer inside the rice plant was measured by serial dilution plating of lysates from budding rice seedlings. Surprisingly, morphologically different types of colonies were observed on the plates. These ‘contaminated’ rice seed-born bacteria (RSB) were identified by sequencing 16S rRNA genes as three strains of Pseudomonas putida (RSB1, RSB10, RSB15) and Stenotrophomonas maltophilia (RSB2). All bacteria and B. glumae were simultaneously inoculated onto rice seeds, and all three P. putida RSBs suppressed the growth disruption caused by B. glumae, whereas RSB2 had no effect. Thus, the virulence was synergistically suppressed when co-treated with RSBs. The effect could be dependent on the high biofilm formation ability of RSB2. By comprehensive microbiota analysis, endogenous rice flora were changed by RSBs treatment. These results suggest the possibility of novel pathogen control through pre-treatment with endogenous beneficial microorganisms. The method would contribute substantially to the implementation of sustainable agriculture stated in Sustainable Development Goals of United Nations.Chiharu Akimoto-TomiyamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chiharu Akimoto-Tomiyama Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae
description	Abstract Burkholderia glumae is a causal agent of bacterial grain and seedling rot in rice, and is a threat to stable global food supply. The virulence of B. glumae was suppressed when it was inoculated on budding seed rather than on non-budding seed. To clarify the phenomena, pathogen titer inside the rice plant was measured by serial dilution plating of lysates from budding rice seedlings. Surprisingly, morphologically different types of colonies were observed on the plates. These ‘contaminated’ rice seed-born bacteria (RSB) were identified by sequencing 16S rRNA genes as three strains of Pseudomonas putida (RSB1, RSB10, RSB15) and Stenotrophomonas maltophilia (RSB2). All bacteria and B. glumae were simultaneously inoculated onto rice seeds, and all three P. putida RSBs suppressed the growth disruption caused by B. glumae, whereas RSB2 had no effect. Thus, the virulence was synergistically suppressed when co-treated with RSBs. The effect could be dependent on the high biofilm formation ability of RSB2. By comprehensive microbiota analysis, endogenous rice flora were changed by RSBs treatment. These results suggest the possibility of novel pathogen control through pre-treatment with endogenous beneficial microorganisms. The method would contribute substantially to the implementation of sustainable agriculture stated in Sustainable Development Goals of United Nations.
format	article
author	Chiharu Akimoto-Tomiyama
author_facet	Chiharu Akimoto-Tomiyama
author_sort	Chiharu Akimoto-Tomiyama
title	Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae
title_short	Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae
title_full	Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae
title_fullStr	Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae
title_full_unstemmed	Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae
title_sort	multiple endogenous seed-born bacteria recovered rice growth disruption caused by burkholderia glumae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2e39e6daa2e14fe1a3139bc6ea5d7e41
work_keys_str_mv	AT chiharuakimototomiyama multipleendogenousseedbornbacteriarecoveredricegrowthdisruptioncausedbyburkholderiaglumae
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Multiple endogenous seed-born bacteria recovered rice growth disruption caused by Burkholderia glumae

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