3D projection electrophoresis for single-cell immunoblotting

Single-cell immunoblotting previously separated proteins on a polyacrylamide slab in the xy direction and was limited by throughput and sample consumption. Here the authors adapt the system to separate proteins in the z direction, allowing for closer spacing of sample wells and improved sample consu...

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Auteurs principaux: Samantha M. Grist, Andoni P. Mourdoukoutas, Amy E. Herr
Format: article
Langue:EN
Publié: Nature Portfolio 2020
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Accès en ligne:https://doaj.org/article/2e3c86ebf1d34b6fa32c5f5fcd0d15a1
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Résumé:Single-cell immunoblotting previously separated proteins on a polyacrylamide slab in the xy direction and was limited by throughput and sample consumption. Here the authors adapt the system to separate proteins in the z direction, allowing for closer spacing of sample wells and improved sample consumption.