3D projection electrophoresis for single-cell immunoblotting

Single-cell immunoblotting previously separated proteins on a polyacrylamide slab in the xy direction and was limited by throughput and sample consumption. Here the authors adapt the system to separate proteins in the z direction, allowing for closer spacing of sample wells and improved sample consu...

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Autores principales:	Samantha M. Grist, Andoni P. Mourdoukoutas, Amy E. Herr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2e3c86ebf1d34b6fa32c5f5fcd0d15a1
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id	oai:doaj.org-article:2e3c86ebf1d34b6fa32c5f5fcd0d15a1
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spelling	oai:doaj.org-article:2e3c86ebf1d34b6fa32c5f5fcd0d15a12021-12-02T15:37:09Z3D projection electrophoresis for single-cell immunoblotting10.1038/s41467-020-19738-12041-1723https://doaj.org/article/2e3c86ebf1d34b6fa32c5f5fcd0d15a12020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19738-1https://doaj.org/toc/2041-1723Single-cell immunoblotting previously separated proteins on a polyacrylamide slab in the xy direction and was limited by throughput and sample consumption. Here the authors adapt the system to separate proteins in the z direction, allowing for closer spacing of sample wells and improved sample consumption.Samantha M. GristAndoni P. MourdoukoutasAmy E. HerrNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Samantha M. Grist Andoni P. Mourdoukoutas Amy E. Herr 3D projection electrophoresis for single-cell immunoblotting
description	Single-cell immunoblotting previously separated proteins on a polyacrylamide slab in the xy direction and was limited by throughput and sample consumption. Here the authors adapt the system to separate proteins in the z direction, allowing for closer spacing of sample wells and improved sample consumption.
format	article
author	Samantha M. Grist Andoni P. Mourdoukoutas Amy E. Herr
author_facet	Samantha M. Grist Andoni P. Mourdoukoutas Amy E. Herr
author_sort	Samantha M. Grist
title	3D projection electrophoresis for single-cell immunoblotting
title_short	3D projection electrophoresis for single-cell immunoblotting
title_full	3D projection electrophoresis for single-cell immunoblotting
title_fullStr	3D projection electrophoresis for single-cell immunoblotting
title_full_unstemmed	3D projection electrophoresis for single-cell immunoblotting
title_sort	3d projection electrophoresis for single-cell immunoblotting
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2e3c86ebf1d34b6fa32c5f5fcd0d15a1
work_keys_str_mv	AT samanthamgrist 3dprojectionelectrophoresisforsinglecellimmunoblotting AT andonipmourdoukoutas 3dprojectionelectrophoresisforsinglecellimmunoblotting AT amyeherr 3dprojectionelectrophoresisforsinglecellimmunoblotting
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3D projection electrophoresis for single-cell immunoblotting

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